Purified anti-mouse IL-12/IL-23 p40 | BioLegend 505201 product information

product summary

company name :

BioLegend

other brands :

Babco, Signet, Sternberger Monoclonals, Senetek, Covance

product type :

antibody

product name :

Purified anti-mouse IL-12/IL-23 p40

catalog :

505201

quantity :

50 μg

price :

70 USD

clonality :

monoclonal

host :

rat

conjugate :

nonconjugated

clone name :

C15.6

reactivity :

mouse

application :

western blot, ELISA, immunoprecipitation, flow cytometry, immunohistochemistry - frozen section

more info or order :

BioLegend product webpage

citations: 8

Published Application/Species/Sample/Dilution	Reference
flow cytometry; mouse; fig 5	Sharma S, Chintala N, Vadrevu S, Patel J, Karbowniczek M, Markiewski M. Pulmonary alveolar macrophages contribute to the premetastatic niche by suppressing antitumor T cell responses in the lungs. J Immunol. 2015;194:5529-38 pubmed publisher
ELISA; mouse; fig 6	Li J, Liu D, Mou Z, Ihedioha O, Blanchard A, Jia P, et al. Deficiency of prolactin-inducible protein leads to impaired Th1 immune response and susceptibility to Leishmania major in mice. Eur J Immunol. 2015;45:1082-91 pubmed publisher
	Kim S, Saeidi S, Zhong X, Gwak S, Muna I, Park S, et al. Breast cancer cell debris diminishes therapeutic efficacy through heme oxygenase-1-mediated inactivation of M1-like tumor-associated macrophages. Neoplasia. 2020;22:606-616 pubmed publisher
	Etxeberria I, Bolaños E, Quetglas J, Gros A, Villanueva A, Palomero J, et al. Intratumor Adoptive Transfer of IL-12 mRNA Transiently Engineered Antitumor CD8+ T Cells. Cancer Cell. 2019;36:613-629.e7 pubmed publisher
	Li H, Yao Q, Mariscal A, Wu X, Hulse J, Pedersen E, et al. Epigenetic control of IL-23 expression in keratinocytes is important for chronic skin inflammation. Nat Commun. 2018;9:1420 pubmed publisher
	Pushalkar S, Hundeyin M, Daley D, Zambirinis C, Kurz E, Mishra A, et al. The Pancreatic Cancer Microbiome Promotes Oncogenesis by Induction of Innate and Adaptive Immune Suppression. Cancer Discov. 2018;8:403-416 pubmed publisher
	Malu D, Bélanger B, Desautels F, Kelendji K, Dalko E, Sanchez Dardon J, et al. Macrophage migration inhibitory factor: a downregulator of early T cell-dependent IFN-gamma responses in Plasmodium chabaudi adami (556 KA)-infected mice. J Immunol. 2011;186:6271-9 pubmed publisher
	Xu G, Liu D, Fan Y, Yang X, Korner H, Fu Y, et al. Lymphotoxin alpha beta 2 (membrane lymphotoxin) is critically important for resistance to Leishmania major infection in mice. J Immunol. 2007;179:5358-66 pubmed

product information

Antigen :

IL-12 IL-23 p40

Apps. Abbrev. :

ELISA Capture, IHC-F, IP, Western Blotting

Cat # :

505201

Clone :

C15.6

Item :

Purified anti-mouse IL-12/IL-23 p40

Isotype :

Rat IgG1, κ

Other Names :

Interleukin-12 p40, Interleukin-23 p40, Cytotoxic lymphocyte maturation factor (CLMF), Natural killer cell stimulatory factor (NKSF), CTL maturation factor (TcMF), T-cell stimulating factor (TSF)

Size :

50 μg

Price (USD) :

70 USD

Reactivity :

Mouse, IL-12/IL-23 p40 subunit (monomer, homodimer and heterodimer IL-12 p35/p40 or IL-23 p19/p40)

Clonality :

Monoclonal

Host :

Rat

Conjugate/Tag/Label :

PURE

Immunogen :

CHO-expressed, recombinant mouse IL-12 p70

Application Notes :

ELISA or ELISPOT Capture 1,2,4,6,8,10 : The purified C15.6 antibody is useful as the capture antibody in a sandwich ELISA or ELISPOT assay, when used in conjunction with the biotinylated C17.8 (Cat. No. 505302) antibody as the detecting antibody. This assay detects p40 as monomer, homodimer, or heterodimer complexed with p35. The LEAF™ purified antibody is suggested for ELISPOT capture. Additional reported applications (for the relevant formats) include: immunohistochemical staining 9 of paraformaldehyde-fixed saponin-treated frozen tissue sections, immunoprecipitation 3 , and Western blotting 3 . Note: For testing mouse IL-12 p40 (monomer, dimer, heteromer) in serum or plasma, BioLegend s ELISA Max™ Sets (Cat. No. 431601 to 431606) are specially developed and recommended.

more info or order :

BioLegend product webpage