product summary
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company name :
BioLegend
other brands :
Babco, Signet, Sternberger Monoclonals, Senetek, Covance
product type :
antibody
product name :
PE/Dazzle™ 594 anti-mouse IL-10
catalog :
505033
quantity :
25 μg
price :
136 USD
clonality :
monoclonal
host :
rat
conjugate :
PE
clone name :
JES5-16E3
reactivity :
human, mouse
application :
flow cytometry
more info or order :
citations: 9
Published Application/Species/Sample/DilutionReference
  • flow cytometry; mouse; 1:50; loading ...; fig s3b
Yang C, Lei L, Collins J, Briones M, Ma L, Sturdevant G, et al. Chlamydia evasion of neutrophil host defense results in NLRP3 dependent myeloid-mediated sterile inflammation through the purinergic P2X7 receptor. Nat Commun. 2021;12:5454 pubmed publisher
  • flow cytometry; mouse; loading ...; fig s4c
Thiele Née Schrewe L, Guse K, Tietz S, Remlinger J, Demir S, Pedreiturria X, et al. Functional relevance of the multi-drug transporter abcg2 on teriflunomide therapy in an animal model of multiple sclerosis. J Neuroinflammation. 2020;17:9 pubmed publisher
  • flow cytometry; mouse; loading ...; fig s2
Kitada S, Kayama H, Okuzaki D, Koga R, Kobayashi M, Arima Y, et al. BATF2 inhibits immunopathological Th17 responses by suppressing Il23a expression during Trypanosoma cruzi infection. J Exp Med. 2017;214:1313-1331 pubmed publisher
  • flow cytometry; human; loading ...; fig 5f
Xie L, Zhou F, Liu X, Fang Y, Yu Z, Song N, et al. Serum microRNA181a: Correlates with the intracellular cytokine levels and a potential biomarker for acute graft-versus-host disease. Cytokine. 2016;85:37-44 pubmed publisher
Palathingal Bava E, George J, Tarique M, Iyer S, Sahay P, Gomez Aguilar B, et al. Pirfenidone increases IL-10 and improves acute pancreatitis in multiple clinically relevant murine models. JCI Insight. 2022;7: pubmed publisher
Pfirschke C, Engblom C, Gungabeesoon J, Lin Y, Rickelt S, Zilionis R, et al. Tumor-Promoting Ly-6G+ SiglecFhigh Cells Are Mature and Long-Lived Neutrophils. Cell Rep. 2020;32:108164 pubmed publisher
Xu G, Liu D, Fan Y, Yang X, Korner H, Fu Y, et al. Lymphotoxin alpha beta 2 (membrane lymphotoxin) is critically important for resistance to Leishmania major infection in mice. J Immunol. 2007;179:5358-66 pubmed
Kang Y, Yang S, Park G, Cho B, Min C, Kim T, et al. A novel function of interleukin-10 promoting self-renewal of hematopoietic stem cells. Stem Cells. 2007;25:1814-22 pubmed
Brummel R, Lenert P. Activation of marginal zone B cells from lupus mice with type A(D) CpG-oligodeoxynucleotides. J Immunol. 2005;174:2429-34 pubmed
product information
Antigen :
IL-10
Apps. Abbrev. :
Intracellular Staining for Flow Cytometry
Cat # :
505033
Clone :
JES5-16E3
Item :
PE/Dazzle™ 594 anti-mouse IL-10
Isotype :
Rat IgG2b, κ
Other Names :
Interleukin-10, Cytokine synthesis inhibitory factor (CSIF), B cell derived T cell growth factor (B-TCGF)
Size :
25 μg
Price (USD) :
136 USD
Reactivity :
Mouse
Clonality :
Monoclonal
Host :
Rat
Conjugate/Tag/Label :
PE_DZL594
Immunogen :
E. coli -expressed, recombinant mouse IL-10
Application Notes :
ELISA or ELISPOT Detection 1,9,11 : The biotinylated JES5-16E3 antibody is useful as a detection antibody for a sandwich ELISA or ELISPOT assay, when used in conjunction with purified JES5-2A5 antibody (Cat. Nos. 504902 504904) as the capture antibody. ELISA Capture : The purified JES5-16E3 antibody is useful as the capture antibody in a sandwich ELISA when used in conjunction with the biotinylated JES5-2A5 antibody (Cat. No. 505003) as the detection antibody and recombinant mouse IL-10 (Cat. No. 575809) as the standard. Neutralization 14 : The Ultra-LEAF™ purified JES5-16E3 antibody can neutralize the bioactivity of natural or recombinant IL-10. Flow Cytometry 3 : The fluorochrome-labeled JES5-16E3 antibody is useful for intracellular immunofluorescent staining and flow cytometric analysis to identify IL-10-producing cells within mixed cell populations. Additional reported applications (for relevant formats) include: immunohistochemistry 3 .
more info or order :
company information
BioLegend
8999 BioLegend Way
San Diego, CA 92121
customerserv@biolegend.com
https://www.biolegend.com
headquarters: USA