PE/Cyanine7 anti-mouse IL-10 | BioLegend 505025 product information

product summary

company name :

BioLegend

other brands :

Babco, Signet, Sternberger Monoclonals, Senetek, Covance

product type :

antibody

product name :

PE/Cyanine7 anti-mouse IL-10

catalog :

505025

quantity :

25 μg

price :

105 USD

clonality :

monoclonal

host :

rat

conjugate :

PE/Cy7

clone name :

JES5-16E3

reactivity :

mouse

application :

flow cytometry

more info or order :

BioLegend product webpage

citations: 12

Published Application/Species/Sample/Dilution	Reference
flow cytometry; mouse	OlguÃn J, FernÃ¡ndez J, Salinas N, JuÃ¡rez I, Rodriguez Sosa M, Campuzano J, et al. Adoptive transfer of CD4(+)Foxp3(+) regulatory T cells to C57BL/6J mice during acute infection with Toxoplasma gondii down modulates the exacerbated Th1 immune response. Microbes Infect. 2015;17:586-95 pubmed publisher
	Li S, Liu Q, Wu D, He T, Yuan J, Qiu H, et al. PKC-δ deficiency in B cells displays osteopenia accompanied with upregulation of RANKL expression and osteoclast-osteoblast uncoupling. Cell Death Dis. 2020;11:762 pubmed publisher
	Sidwell T, Liao Y, Garnham A, Vasanthakumar A, Gloury R, Blume J, et al. Attenuation of TCR-induced transcription by Bach2 controls regulatory T cell differentiation and homeostasis. Nat Commun. 2020;11:252 pubmed publisher
	Amir M, Chaudhari S, Wang R, Campbell S, Mosure S, Chopp L, et al. REV-ERBα Regulates TH17 Cell Development and Autoimmunity. Cell Rep. 2018;25:3733-3749.e8 pubmed publisher
	Bouziat R, Biering S, Kouame E, Sangani K, Kang S, Ernest J, et al. Murine Norovirus Infection Induces TH1 Inflammatory Responses to Dietary Antigens. Cell Host Microbe. 2018;24:677-688.e5 pubmed publisher
	Wei C, Mei J, Tang L, Liu Y, Li D, Li M, et al. 1-Methyl-tryptophan attenuates regulatory T cells differentiation due to the inhibition of estrogen-IDO1-MRC2 axis in endometriosis. Cell Death Dis. 2016;7:e2489 pubmed publisher
	Delmas A, Oikonomopoulos A, Lacey P, Fallahi M, Hommes D, Sundrud M. Informatics-Based Discovery of Disease-Associated Immune Profiles. PLoS ONE. 2016;11:e0163305 pubmed publisher
	D Ambrosio A, Cossu A, Amendola A, Zandri A, Butera A, Sanchez M, et al. Lamina Propria CD4+LAP+ Regulatory T Cells Are Increased in Active Ulcerative Colitis but Show Increased IL-17 Expression and Reduced Suppressor Activity. J Crohns Colitis. 2016;10:346-53 pubmed publisher
	Vossenkamper A, Hundsrucker C, Page K, van Maurik A, Sanders T, Stagg A, et al. A CD3-specific antibody reduces cytokine production and alters phosphoprotein profiles in intestinal tissues from patients with inflammatory bowel disease. Gastroenterology. 2014;147:172-83 pubmed publisher
	Xu G, Liu D, Fan Y, Yang X, Korner H, Fu Y, et al. Lymphotoxin alpha beta 2 (membrane lymphotoxin) is critically important for resistance to Leishmania major infection in mice. J Immunol. 2007;179:5358-66 pubmed
	Kang Y, Yang S, Park G, Cho B, Min C, Kim T, et al. A novel function of interleukin-10 promoting self-renewal of hematopoietic stem cells. Stem Cells. 2007;25:1814-22 pubmed
	Brummel R, Lenert P. Activation of marginal zone B cells from lupus mice with type A(D) CpG-oligodeoxynucleotides. J Immunol. 2005;174:2429-34 pubmed

product information

Antigen :

IL-10

Apps. Abbrev. :

Intracellular Staining for Flow Cytometry

Cat # :

505025

Clone :

JES5-16E3

Item :

PE/Cyanine7 anti-mouse IL-10

Isotype :

Rat IgG2b, κ

Other Names :

Interleukin-10, Cytokine synthesis inhibitory factor (CSIF), B cell derived T cell growth factor (B-TCGF)

Size :

25 μg

Price (USD) :

105 USD

Reactivity :

Mouse

Clonality :

Monoclonal

Host :

Rat

Conjugate/Tag/Label :

PE_CYANINE7

Immunogen :

E. coli -expressed, recombinant mouse IL-10

Application Notes :

ELISA or ELISPOT Detection 1,9,11 : The biotinylated JES5-16E3 antibody is useful as a detection antibody for a sandwich ELISA or ELISPOT assay, when used in conjunction with purified JES5-2A5 antibody (Cat. Nos. 504902 504904) as the capture antibody. ELISA Capture : The purified JES5-16E3 antibody is useful as the capture antibody in a sandwich ELISA when used in conjunction with the biotinylated JES5-2A5 antibody (Cat. No. 505003) as the detection antibody and recombinant mouse IL-10 (Cat. No. 575809) as the standard. Neutralization 14 : The Ultra-LEAF™ purified JES5-16E3 antibody can neutralize the bioactivity of natural or recombinant IL-10. Flow Cytometry 3 : The fluorochrome-labeled JES5-16E3 antibody is useful for intracellular immunofluorescent staining and flow cytometric analysis to identify IL-10-producing cells within mixed cell populations. Additional reported applications (for relevant formats) include: immunohistochemistry 3 .

more info or order :

BioLegend product webpage