product summary
Loading...
company name :
BioLegend
other brands :
Babco, Signet, Sternberger Monoclonals, Senetek, Covance
product type :
antibody
product name :
Alexa Fluor® 647 anti-mouse IL-10
catalog :
505014
quantity :
100 μg
price :
248 USD
clonality :
monoclonal
host :
rat
conjugate :
AF647
clone name :
JES5-16E3
reactivity :
human, mouse
application :
flow cytometry
more info or order :
citations: 37
Published Application/Species/Sample/DilutionReference
  • flow cytometry; mouse; loading ...; fig 4c
Zhang M, Pan X, Fujiwara K, Jurcak N, Muth S, Zhou J, et al. Pancreatic cancer cells render tumor-associated macrophages metabolically reprogrammed by a GARP and DNA methylation-mediated mechanism. Signal Transduct Target Ther. 2021;6:366 pubmed publisher
  • flow cytometry; mouse; 1:50; loading ...; fig s3b
Yang C, Lei L, Collins J, Briones M, Ma L, Sturdevant G, et al. Chlamydia evasion of neutrophil host defense results in NLRP3 dependent myeloid-mediated sterile inflammation through the purinergic P2X7 receptor. Nat Commun. 2021;12:5454 pubmed publisher
  • flow cytometry; mouse; 1:100; loading ...; fig 1c
Amoozgar Z, Kloepper J, Ren J, Tay R, Kazer S, Kiner E, et al. Targeting Treg cells with GITR activation alleviates resistance to immunotherapy in murine glioblastomas. Nat Commun. 2021;12:2582 pubmed publisher
  • flow cytometry; mouse; loading ...; fig s4c
Thiele Née Schrewe L, Guse K, Tietz S, Remlinger J, Demir S, Pedreiturria X, et al. Functional relevance of the multi-drug transporter abcg2 on teriflunomide therapy in an animal model of multiple sclerosis. J Neuroinflammation. 2020;17:9 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 1d
Tanaka S, Pfleger C, Lai J, Roan F, Sun S, Ziegler S. KAP1 Regulates Regulatory T Cell Function and Proliferation in Both Foxp3-Dependent and -Independent Manners. Cell Rep. 2018;23:796-807 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 6a
Varelias A, Bunting M, Ormerod K, Koyama M, Olver S, Straube J, et al. Recipient mucosal-associated invariant T cells control GVHD within the colon. J Clin Invest. 2018;128:1919-1936 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 6b
Daley D, Mani V, Mohan N, Akkad N, Pandian G, Savadkar S, et al. NLRP3 signaling drives macrophage-induced adaptive immune suppression in pancreatic carcinoma. J Exp Med. 2017;214:1711-1724 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 3e
Daley D, Mani V, Mohan N, Akkad N, Ochi A, Heindel D, et al. Dectin 1 activation on macrophages by galectin 9 promotes pancreatic carcinoma and peritumoral immune tolerance. Nat Med. 2017;23:556-567 pubmed publisher
  • flow cytometry; mouse; loading ...; fig s2
Kitada S, Kayama H, Okuzaki D, Koga R, Kobayashi M, Arima Y, et al. BATF2 inhibits immunopathological Th17 responses by suppressing Il23a expression during Trypanosoma cruzi infection. J Exp Med. 2017;214:1313-1331 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 3
Ulrich B, Verdan F, McKenzie A, Kaplan M, Olson M. STAT3 Activation Impairs the Stability of Th9 Cells. J Immunol. 2017;198:2302-2309 pubmed publisher
  • flow cytometry; human; loading ...; fig 5f
Xie L, Zhou F, Liu X, Fang Y, Yu Z, Song N, et al. Serum microRNA181a: Correlates with the intracellular cytokine levels and a potential biomarker for acute graft-versus-host disease. Cytokine. 2016;85:37-44 pubmed publisher
  • flow cytometry; mouse
Lu K, Keppler S, Leithäuser F, Mattfeldt T, Castello A, Kostezka U, et al. Nck adaptor proteins modulate differentiation and effector function of T cells. J Leukoc Biol. 2015;98:301-11 pubmed publisher
Pasciuto E, Burton O, Roca C, Lagou V, Rajan W, Theys T, et al. Microglia Require CD4 T Cells to Complete the Fetal-to-Adult Transition. Cell. 2020;182:625-640.e24 pubmed publisher
Roux C, Jafari S, Shinde R, Duncan G, Cescon D, Silvester J, et al. Reactive oxygen species modulate macrophage immunosuppressive phenotype through the up-regulation of PD-L1. Proc Natl Acad Sci U S A. 2019;: pubmed publisher
Das S, Bar Sagi D. BTK signaling drives CD1dhiCD5+ regulatory B-cell differentiation to promote pancreatic carcinogenesis. Oncogene. 2019;38:3316-3324 pubmed publisher
Jain R, Parham K, Tesfagiorgis Y, Craig H, Romanchik E, Kerfoot S. Autoreactive, Low-Affinity T Cells Preferentially Drive Differentiation of Short-Lived Memory B Cells at the Expense of Germinal Center Maintenance. Cell Rep. 2018;25:3342-3355.e5 pubmed publisher
Edwards C, de Oca M, de Labastida Rivera F, Kumar R, Ng S, Wang Y, et al. The Role of BACH2 in T Cells in Experimental Malaria Caused by Plasmodium chabaudi chabaudi AS. Front Immunol. 2018;9:2578 pubmed publisher
Vaibhav K, Braun M, Khan M, Fatima S, Saad N, Shankar A, et al. Remote ischemic post-conditioning promotes hematoma resolution via AMPK-dependent immune regulation. J Exp Med. 2018;215:2636-2654 pubmed publisher
He Y, Yuan X, Li Y, Zhong C, Liu Y, Qian H, et al. Loss of Gαq impairs regulatory B-cell function. Arthritis Res Ther. 2018;20:186 pubmed publisher
Li J, Byrne K, Yan F, Yamazoe T, Chen Z, Baslan T, et al. Tumor Cell-Intrinsic Factors Underlie Heterogeneity of Immune Cell Infiltration and Response to Immunotherapy. Immunity. 2018;49:178-193.e7 pubmed publisher
Stüve P, Minarrieta L, Erdmann H, Arnold Schrauf C, Swallow M, Guderian M, et al. De Novo Fatty Acid Synthesis During Mycobacterial Infection Is a Prerequisite for the Function of Highly Proliferative T Cells, But Not for Dendritic Cells or Macrophages. Front Immunol. 2018;9:495 pubmed publisher
Mencarelli A, Vacca M, Khameneh H, Acerbi E, Tay A, Zolezzi F, et al. Calcineurin B in CD4+ T Cells Prevents Autoimmune Colitis by Negatively Regulating the JAK/STAT Pathway. Front Immunol. 2018;9:261 pubmed publisher
Yu J, Duong V, Westphal K, Westphal A, Suwandi A, Grassl G, et al. Surface receptor Toso controls B cell-mediated regulation of T cell immunity. J Clin Invest. 2018;128:1820-1836 pubmed publisher
SMITH C, Allard D, Wang Y, Howard J, Montgomery S, Su M. IL-10 Paradoxically Promotes Autoimmune Neuropathy through S1PR1-Dependent CD4+ T Cell Migration. J Immunol. 2018;200:1580-1592 pubmed publisher
Wu J, Zhang H, Shi X, Xiao X, Fan Y, Minze L, et al. Ablation of Transcription Factor IRF4 Promotes Transplant Acceptance by Driving Allogenic CD4+ T Cell Dysfunction. Immunity. 2017;47:1114-1128.e6 pubmed publisher
Wang Q, Racine J, Ratiu J, Wang S, Ettinger R, Wasserfall C, et al. Transient BAFF Blockade Inhibits Type 1 Diabetes Development in Nonobese Diabetic Mice by Enriching Immunoregulatory B Lymphocytes Sensitive to Deletion by Anti-CD20 Cotherapy. J Immunol. 2017;199:3757-3770 pubmed publisher
Clay G, Valadares D, Graff J, Ulland T, Davis R, Scorza B, et al. An Anti-Inflammatory Role for NLRP10 in Murine Cutaneous Leishmaniasis. J Immunol. 2017;199:2823-2833 pubmed publisher
Nasti A, Sakai Y, Seki A, Buffa G, Komura T, Mochida H, et al. The CD45+ fraction in murine adipose tissue derived stromal cells harbors immune-inhibitory inflammatory cells. Eur J Immunol. 2017;47:2163-2174 pubmed publisher
Novince C, Whittow C, Aartun J, Hathaway J, Poulides N, Chavez M, et al. Commensal Gut Microbiota Immunomodulatory Actions in Bone Marrow and Liver have Catabolic Effects on Skeletal Homeostasis in Health. Sci Rep. 2017;7:5747 pubmed publisher
Suzuki T, Murakami S, Biswal S, Sakaguchi S, Harigae H, Yamamoto M, et al. Systemic Activation of NRF2 Alleviates Lethal Autoimmune Inflammation in Scurfy Mice. Mol Cell Biol. 2017;37: pubmed publisher
Soto R, Petersen C, Novis C, Kubinak J, Bell R, Stephens W, et al. Microbiota promotes systemic T-cell survival through suppression of an apoptotic factor. Proc Natl Acad Sci U S A. 2017;114:5497-5502 pubmed publisher
Schweighoffer E, Nys J, Vanes L, Smithers N, Tybulewicz V. TLR4 signals in B lymphocytes are transduced via the B cell antigen receptor and SYK. J Exp Med. 2017;214:1269-1280 pubmed publisher
Jensen H, Chen S, Folkersen L, Nolan G, Lanier L. EBI3 regulates the NK cell response to mouse cytomegalovirus infection. Proc Natl Acad Sci U S A. 2017;114:1625-1630 pubmed publisher
del Rio R, Noubade R, Saligrama N, Wall E, Krementsov D, Poynter M, et al. Histamine H4 receptor optimizes T regulatory cell frequency and facilitates anti-inflammatory responses within the central nervous system. J Immunol. 2012;188:541-7 pubmed publisher
Xu G, Liu D, Fan Y, Yang X, Korner H, Fu Y, et al. Lymphotoxin alpha beta 2 (membrane lymphotoxin) is critically important for resistance to Leishmania major infection in mice. J Immunol. 2007;179:5358-66 pubmed
Kang Y, Yang S, Park G, Cho B, Min C, Kim T, et al. A novel function of interleukin-10 promoting self-renewal of hematopoietic stem cells. Stem Cells. 2007;25:1814-22 pubmed
Brummel R, Lenert P. Activation of marginal zone B cells from lupus mice with type A(D) CpG-oligodeoxynucleotides. J Immunol. 2005;174:2429-34 pubmed
product information
Antigen :
IL-10
Apps. Abbrev. :
Intracellular Staining for Flow Cytometry
Cat # :
505014
Clone :
JES5-16E3
Item :
Alexa Fluor® 647 anti-mouse IL-10
Isotype :
Rat IgG2b, κ
Other Names :
Interleukin-10, Cytokine synthesis inhibitory factor (CSIF), B cell derived T cell growth factor (B-TCGF)
Size :
100 μg
Price (USD) :
248 USD
Reactivity :
Mouse
Clonality :
Monoclonal
Host :
Rat
Conjugate/Tag/Label :
A647
Immunogen :
E. coli -expressed, recombinant mouse IL-10
Application Notes :
ELISA or ELISPOT Detection 1,9,11 : The biotinylated JES5-16E3 antibody is useful as a detection antibody for a sandwich ELISA or ELISPOT assay, when used in conjunction with purified JES5-2A5 antibody (Cat. Nos. 504902 504904) as the capture antibody. ELISA Capture : The purified JES5-16E3 antibody is useful as the capture antibody in a sandwich ELISA when used in conjunction with the biotinylated JES5-2A5 antibody (Cat. No. 505003) as the detection antibody and recombinant mouse IL-10 (Cat. No. 575809) as the standard. Neutralization 14 : The Ultra-LEAF™ purified JES5-16E3 antibody can neutralize the bioactivity of natural or recombinant IL-10. Flow Cytometry 3 : The fluorochrome-labeled JES5-16E3 antibody is useful for intracellular immunofluorescent staining and flow cytometric analysis to identify IL-10-producing cells within mixed cell populations. Additional reported applications (for relevant formats) include: immunohistochemistry 3 .
more info or order :
company information
BioLegend
8999 BioLegend Way
San Diego, CA 92121
customerserv@biolegend.com
https://www.biolegend.com
headquarters: USA