APC anti-mouse IL-10 | BioLegend 505009 product information

product summary

company name :

BioLegend

other brands :

Babco, Signet, Sternberger Monoclonals, Senetek, Covance

product type :

antibody

product name :

APC anti-mouse IL-10

catalog :

505009

quantity :

25 μg

price :

85 USD

clonality :

monoclonal

host :

rat

conjugate :

APC

clone name :

JES5-16E3

reactivity :

mouse

application :

flow cytometry

more info or order :

BioLegend product webpage

citations: 20

Published Application/Species/Sample/Dilution	Reference
flow cytometry; mouse; loading ...	Schweighoffer E, Nys J, Vanes L, Smithers N, Tybulewicz V. TLR4 signals in B lymphocytes are transduced via the B cell antigen receptor and SYK. J Exp Med. 2017;214:1269-1280 pubmed publisher
	Yang W, Yu T, Huang X, Bilotta A, Xu L, Lu Y, et al. Intestinal microbiota-derived short-chain fatty acids regulation of immune cell IL-22 production and gut immunity. Nat Commun. 2020;11:4457 pubmed publisher
	Qin J, Zhao N, Wang S, Liu S, Liu Y, Cui X, et al. Roles of Endogenous IL-10 and IL-10-Competent and CD5+ B Cells in Autoimmune Thyroiditis in NOD.H-2h4 Mice. Endocrinology. 2020;161: pubmed publisher
	Chao C, Gutiérrez Vázquez C, Rothhammer V, Mayo L, Wheeler M, Tjon E, et al. Metabolic Control of Astrocyte Pathogenic Activity via cPLA2-MAVS. Cell. 2019;179:1483-1498.e22 pubmed publisher
	Yadava K, Medina C, Ishak H, Gurevich I, Kuipers H, Shamskhou E, et al. Natural Tr1-like cells do not confer long-term tolerogenic memory. elife. 2019;8: pubmed publisher
	Komuczki J, Tuzlak S, Friebel E, Hartwig T, Spath S, Rosenstiel P, et al. Fate-Mapping of GM-CSF Expression Identifies a Discrete Subset of Inflammation-Driving T Helper Cells Regulated by Cytokines IL-23 and IL-1β. Immunity. 2019;: pubmed publisher
	Burzynski L, Humphry M, Pyrillou K, Wiggins K, Chan J, Figg N, et al. The Coagulation and Immune Systems Are Directly Linked through the Activation of Interleukin-1α by Thrombin. Immunity. 2019;50:1033-1042.e6 pubmed publisher
	Wheeler M, Jaronen M, Covacu R, Zandee S, Scalisi G, Rothhammer V, et al. Environmental Control of Astrocyte Pathogenic Activities in CNS Inflammation. Cell. 2019;176:581-596.e18 pubmed publisher
	Das S, Bar Sagi D. BTK signaling drives CD1dhiCD5+ regulatory B-cell differentiation to promote pancreatic carcinogenesis. Oncogene. 2019;38:3316-3324 pubmed publisher
	Ibrahim M, Klement J, Lu C, Redd P, Xiao W, Yang D, et al. Myeloid-Derived Suppressor Cells Produce IL-10 to Elicit DNMT3b-Dependent IRF8 Silencing to Promote Colitis-Associated Colon Tumorigenesis. Cell Rep. 2018;25:3036-3046.e6 pubmed publisher
	Vaibhav K, Braun M, Khan M, Fatima S, Saad N, Shankar A, et al. Remote ischemic post-conditioning promotes hematoma resolution via AMPK-dependent immune regulation. J Exp Med. 2018;215:2636-2654 pubmed publisher
	Habib S, El Andaloussi A, Elmasry K, Handoussa A, Azab M, Elsawey A, et al. PDL-1 Blockade Prevents T Cell Exhaustion, Inhibits Autophagy, and Promotes Clearance of Leishmania donovani. Infect Immun. 2018;86: pubmed publisher
	Wang Q, He Z, Huang M, Liu T, Wang Y, Xu H, et al. Vascular niche IL-6 induces alternative macrophage activation in glioblastoma through HIF-2α. Nat Commun. 2018;9:559 pubmed publisher
	Novince C, Whittow C, Aartun J, Hathaway J, Poulides N, Chavez M, et al. Commensal Gut Microbiota Immunomodulatory Actions in Bone Marrow and Liver have Catabolic Effects on Skeletal Homeostasis in Health. Sci Rep. 2017;7:5747 pubmed publisher
	Baglaenko Y, Manion K, Chang N, Gracey E, Loh C, Wither J. IL-10 Production Is Critical for Sustaining the Expansion of CD5+ B and NKT Cells and Restraining Autoantibody Production in Congenic Lupus-Prone Mice. PLoS ONE. 2016;11:e0150515 pubmed publisher
	Colliou N, Sahay B, Zadeh M, Owen J, Mohamadzadeh M. Impact of Gastrointestinal Bacillus anthracis Infection on Hepatic B Cells. Toxins (Basel). 2015;7:3805-17 pubmed publisher
	BÃ¶hm L, Maxeiner J, Meyer Martin H, Reuter S, Finotto S, Klein M, et al. IL-10 and regulatory T cells cooperate in allergen-specific immunotherapy to ameliorate allergic asthma. J Immunol. 2015;194:887-97 pubmed publisher
	Zhao J, Fett C, Pewe L, Zhao J, Perlman S. Development of transgenic mice expressing a coronavirus-specific public CD4 T cell receptor. J Immunol Methods. 2013;396:56-64 pubmed publisher
	Sugiura D, Denda Nagai K, Takashima M, Murakami R, Nagai S, Takeda K, et al. Local effects of regulatory T cells in MUC1 transgenic mice potentiate growth of MUC1 expressing tumor cells in vivo. PLoS ONE. 2012;7:e44770 pubmed publisher
	Bochtler P, Kröger A, Schirmbeck R, Reimann J. Type I IFN-induced, NKT cell-mediated negative control of CD8 T cell priming by dendritic cells. J Immunol. 2008;181:1633-43 pubmed

product information

Antigen :

IL-10

Apps. Abbrev. :

Intracellular Staining for Flow Cytometry

Cat # :

505009

Clone :

JES5-16E3

Item :

APC anti-mouse IL-10

Isotype :

Rat IgG2b, κ

Other Names :

Interleukin-10, Cytokine synthesis inhibitory factor (CSIF), B cell derived T cell growth factor (B-TCGF)

Size :

25 μg

Price (USD) :

85 USD

Reactivity :

Mouse

Clonality :

Monoclonal

Host :

Rat

Conjugate/Tag/Label :

APC

Immunogen :

E. coli -expressed, recombinant mouse IL-10

Application Notes :

ELISA or ELISPOT Detection 1,9,11 : The biotinylated JES5-16E3 antibody is useful as a detection antibody for a sandwich ELISA or ELISPOT assay, when used in conjunction with purified JES5-2A5 antibody (Cat. Nos. 504902 504904) as the capture antibody. ELISA Capture : The purified JES5-16E3 antibody is useful as the capture antibody in a sandwich ELISA when used in conjunction with the biotinylated JES5-2A5 antibody (Cat. No. 505003) as the detection antibody and recombinant mouse IL-10 (Cat. No. 575809) as the standard. Neutralization 14 : The Ultra-LEAF™ purified JES5-16E3 antibody can neutralize the bioactivity of natural or recombinant IL-10. Flow Cytometry 3 : The fluorochrome-labeled JES5-16E3 antibody is useful for intracellular immunofluorescent staining and flow cytometric analysis to identify IL-10-producing cells within mixed cell populations. Additional reported applications (for relevant formats) include: immunohistochemistry 3 .

more info or order :

BioLegend product webpage