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company name :
BioLegend
other brands :
Babco, Signet, Sternberger Monoclonals, Senetek, Covance
product type :
antibody
product name :
PE anti-mouse IL-10
catalog :
505008
quantity :
100 μg
price :
220 USD
clonality :
monoclonal
host :
rat
conjugate :
PE
clone name :
JES5-16E3
reactivity :
human, mouse
application :
flow cytometry
more info or order :
citations: 34
Published Application/Species/Sample/DilutionReference
  • flow cytometry; mouse; 1:50; loading ...; fig s3b
Yang C, Lei L, Collins J, Briones M, Ma L, Sturdevant G, et al. Chlamydia evasion of neutrophil host defense results in NLRP3 dependent myeloid-mediated sterile inflammation through the purinergic P2X7 receptor. Nat Commun. 2021;12:5454 pubmed publisher
  • flow cytometry; mouse; fig 4a
Zheng W, Song H, Luo Z, Wu H, Chen L, Wang Y, et al. Acetylcholine ameliorates colitis by promoting IL-10 secretion of monocytic myeloid-derived suppressor cells through the nAChR/ERK pathway. Proc Natl Acad Sci U S A. 2021;118: pubmed publisher
  • flow cytometry; mouse; loading ...; fig s4c
Thiele Née Schrewe L, Guse K, Tietz S, Remlinger J, Demir S, Pedreiturria X, et al. Functional relevance of the multi-drug transporter abcg2 on teriflunomide therapy in an animal model of multiple sclerosis. J Neuroinflammation. 2020;17:9 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 3c
Garić D, Tao S, Ahmed E, Youssef M, Kanagaratham C, Shah J, et al. Depletion of BAFF cytokine exacerbates infection in Pseudomonas aeruginosa infected mice. J Cyst Fibros. 2019;18:349-356 pubmed publisher
  • flow cytometry; mouse; loading ...; fig s2
Kitada S, Kayama H, Okuzaki D, Koga R, Kobayashi M, Arima Y, et al. BATF2 inhibits immunopathological Th17 responses by suppressing Il23a expression during Trypanosoma cruzi infection. J Exp Med. 2017;214:1313-1331 pubmed publisher
  • flow cytometry; human; loading ...; fig 5f
Xie L, Zhou F, Liu X, Fang Y, Yu Z, Song N, et al. Serum microRNA181a: Correlates with the intracellular cytokine levels and a potential biomarker for acute graft-versus-host disease. Cytokine. 2016;85:37-44 pubmed publisher
An J, Ding Y, Yu C, Li J, You S, Liu Z, et al. AMP-activated protein kinase alpha1 promotes tumor development via FOXP3 elevation in tumor-infiltrating Treg cells. iScience. 2022;25:103570 pubmed publisher
Choi Y, Seong K, Lee H, Lee S, Kim A. Expansion of monocytic myeloid-derived suppressor cells ameliorated intestinal inflammatory response by radiation through SOCS3 expression. Cell Death Dis. 2021;12:826 pubmed publisher
Delacher M, Simon M, Sanderink L, Hotz Wagenblatt A, Wuttke M, Schambeck K, et al. Single-cell chromatin accessibility landscape identifies tissue repair program in human regulatory T cells. Immunity. 2021;54:702-720.e17 pubmed publisher
Xu W, Snell L, Guo M, Boukhaled G, Macleod B, Li M, et al. Early innate and adaptive immune perturbations determine long-term severity of chronic virus and Mycobacterium tuberculosis coinfection. Immunity. 2021;54:526-541.e7 pubmed publisher
Wu B, Zhang S, Guo Z, Bi Y, Zhou M, Li P, et al. The TGF-β superfamily cytokine Activin-A is induced during autoimmune neuroinflammation and drives pathogenic Th17 cell differentiation. Immunity. 2021;54:308-323.e6 pubmed publisher
Soon M, Lee H, Engel J, Straube J, Thomas B, Pernold C, et al. Transcriptome dynamics of CD4+ T cells during malaria maps gradual transit from effector to memory. Nat Immunol. 2020;21:1597-1610 pubmed publisher
Wang N, Yi H, Fang L, Jin J, Ma Q, Shen Y, et al. CD226 Attenuates Treg Proliferation via Akt and Erk Signaling in an EAE Model. Front Immunol. 2020;11:1883 pubmed publisher
Asadi Shahmirzadi A, Edgar D, Liao C, Hsu Y, Lucanic M, Asadi Shahmirzadi A, et al. Alpha-Ketoglutarate, an Endogenous Metabolite, Extends Lifespan and Compresses Morbidity in Aging Mice. Cell Metab. 2020;32:447-456.e6 pubmed publisher
Xiao S, Bod L, Pochet N, KOTA S, Hu D, Madi A, et al. Checkpoint Receptor TIGIT Expressed on Tim-1+ B Cells Regulates Tissue Inflammation. Cell Rep. 2020;32:107892 pubmed publisher
Hewitson J, West K, James K, Rani G, Dey N, Romano A, et al. Malat1 Suppresses Immunity to Infection through Promoting Expression of Maf and IL-10 in Th Cells. J Immunol. 2020;204:2949-2960 pubmed publisher
Thomas A, Dong Y, Beskid N, Garcia A, Adams A, Babensee J. Brief exposure to hyperglycemia activates dendritic cells in vitro and in vivo. J Cell Physiol. 2020;235:5120-5129 pubmed publisher
Sendler M, van den Brandt C, Glaubitz J, Wilden A, Golchert J, Weiss F, et al. NLRP3 Inflammasome Regulates Development of Systemic Inflammatory Response and Compensatory Anti-Inflammatory Response Syndromes in Mice With Acute Pancreatitis. Gastroenterology. 2020;158:253-269.e14 pubmed publisher
Andersen L, Gülich A, Alteneder M, Preglej T, Orola M, Dhele N, et al. The Transcription Factor MAZR/PATZ1 Regulates the Development of FOXP3+ Regulatory T Cells. Cell Rep. 2019;29:4447-4459.e6 pubmed publisher
Piper C, Rosser E, Oleinika K, Nistala K, Krausgruber T, Rendeiro A, et al. Aryl Hydrocarbon Receptor Contributes to the Transcriptional Program of IL-10-Producing Regulatory B Cells. Cell Rep. 2019;29:1878-1892.e7 pubmed publisher
Imbratta C, Leblond M, Bouzourene H, Speiser D, Velin D, Verdeil G. Maf deficiency in T cells dysregulates Treg - TH17 balance leading to spontaneous colitis. Sci Rep. 2019;9:6135 pubmed publisher
Doonan J, Tarafdar A, Pineda M, Lumb F, Crowe J, Khan A, et al. The parasitic worm product ES-62 normalises the gut microbiota bone marrow axis in inflammatory arthritis. Nat Commun. 2019;10:1554 pubmed publisher
Lu Y, Lin Y, Huang X, Wu S, Wei J, Yang C. Oxaliplatin aggravates hepatic oxidative stress, inflammation and fibrosis in a non‑alcoholic fatty liver disease mouse model. Int J Mol Med. 2019;43:2398-2408 pubmed publisher
Jayachandran R, Gumienny A, Bolinger B, Ruehl S, Lang M, Fucile G, et al. Disruption of Coronin 1 Signaling in T Cells Promotes Allograft Tolerance while Maintaining Anti-Pathogen Immunity. Immunity. 2019;50:152-165.e8 pubmed publisher
Habib S, El Andaloussi A, Elmasry K, Handoussa A, Azab M, Elsawey A, et al. PDL-1 Blockade Prevents T Cell Exhaustion, Inhibits Autophagy, and Promotes Clearance of Leishmania donovani. Infect Immun. 2018;86: pubmed publisher
Tritama E, Riani C, Rudiansyah I, Hidayat A, Kharisnaeni S, Retnoningrum D. Evaluation of alum-based adjuvant on the immunogenicity of salmonella enterica serovar typhi conjugates vaccines. Hum Vaccin Immunother. 2018;14:1524-1529 pubmed publisher
Clemente Casares X, Hosseinzadeh S, Barbu I, Dick S, Macklin J, Wang Y, et al. A CD103+ Conventional Dendritic Cell Surveillance System Prevents Development of Overt Heart Failure during Subclinical Viral Myocarditis. Immunity. 2017;47:974-989.e8 pubmed publisher
Wang Y, Jiang X, Zhu J, Dan Yue -, Zhang X, Wang X, et al. IL-21/IL-21R signaling suppresses intestinal inflammation induced by DSS through regulation of Th responses in lamina propria in mice. Sci Rep. 2016;6:31881 pubmed publisher
Kawano H, Kayama H, Nakama T, Hashimoto T, Umemoto E, Takeda K. IL-10-producing lung interstitial macrophages prevent neutrophilic asthma. Int Immunol. 2016;28:489-501 pubmed
Su N, Yue Y, Xiong S. Monocytic myeloid-derived suppressor cells from females, but not males, alleviate CVB3-induced myocarditis by increasing regulatory and CD4(+)IL-10(+) T cells. Sci Rep. 2016;6:22658 pubmed publisher
Singh K, Hjort M, Thorvaldson L, Sandler S. Concomitant analysis of Helios and Neuropilin-1 as a marker to detect thymic derived regulatory T cells in naïve mice. Sci Rep. 2015;5:7767 pubmed publisher
Böhm L, Maxeiner J, Meyer Martin H, Reuter S, Finotto S, Klein M, et al. IL-10 and regulatory T cells cooperate in allergen-specific immunotherapy to ameliorate allergic asthma. J Immunol. 2015;194:887-97 pubmed publisher
Joly M, Martin R, Mitra Kaushik S, Phillips L, D Angona A, Richards S, et al. Transient low-dose methotrexate generates B regulatory cells that mediate antigen-specific tolerance to alglucosidase alfa. J Immunol. 2014;193:3947-58 pubmed publisher
Pina A, de Araujo E, Felonato M, Loures F, Feriotti C, Bernardino S, et al. Myeloid dendritic cells (DCs) of mice susceptible to paracoccidioidomycosis suppress T cell responses whereas myeloid and plasmacytoid DCs from resistant mice induce effector and regulatory T cells. Infect Immun. 2013;81:1064-77 pubmed publisher
product information
Antigen :
IL-10
Apps. Abbrev. :
Intracellular Staining for Flow Cytometry
Cat # :
505008
Clone :
JES5-16E3
Item :
PE anti-mouse IL-10
Isotype :
Rat IgG2b, κ
Other Names :
Interleukin-10, Cytokine synthesis inhibitory factor (CSIF), B cell derived T cell growth factor (B-TCGF)
Size :
100 μg
Price (USD) :
220 USD
Reactivity :
Mouse
Clonality :
Monoclonal
Host :
Rat
Conjugate/Tag/Label :
PE
Immunogen :
E. coli -expressed, recombinant mouse IL-10
Application Notes :
ELISA or ELISPOT Detection 1,9,11 : The biotinylated JES5-16E3 antibody is useful as a detection antibody for a sandwich ELISA or ELISPOT assay, when used in conjunction with purified JES5-2A5 antibody (Cat. Nos. 504902 504904) as the capture antibody. ELISA Capture : The purified JES5-16E3 antibody is useful as the capture antibody in a sandwich ELISA when used in conjunction with the biotinylated JES5-2A5 antibody (Cat. No. 505003) as the detection antibody and recombinant mouse IL-10 (Cat. No. 575809) as the standard. Neutralization 14 : The Ultra-LEAF™ purified JES5-16E3 antibody can neutralize the bioactivity of natural or recombinant IL-10. Flow Cytometry 3 : The fluorochrome-labeled JES5-16E3 antibody is useful for intracellular immunofluorescent staining and flow cytometric analysis to identify IL-10-producing cells within mixed cell populations. Additional reported applications (for relevant formats) include: immunohistochemistry 3 .
more info or order :
company information
BioLegend
8999 BioLegend Way
San Diego, CA 92121
customerserv@biolegend.com
https://www.biolegend.com
headquarters: USA