Purified anti-mouse IL-10 | BioLegend 504901 product information

product summary

company name :

BioLegend

other brands :

Babco, Signet, Sternberger Monoclonals, Senetek, Covance

product type :

antibody

product name :

Purified anti-mouse IL-10

catalog :

504901

quantity :

50 μg

price :

83 USD

clonality :

monoclonal

host :

rat

conjugate :

nonconjugated

clone name :

JES5-2A5

reactivity :

mouse

application :

western blot, ELISA, immunohistochemistry

more info or order :

BioLegend product webpage

citations: 14

Published Application/Species/Sample/Dilution	Reference
immunohistochemistry; mouse; 10 ug/ml	Vuillefroy de Silly R, DucimetiÃ¨re L, Yacoub Maroun C, Dietrich P, Derouazi M, Walker P. Phenotypic switch of CD8(+) T cells reactivated under hypoxia toward IL-10 secreting, poorly proliferative effector cells. Eur J Immunol. 2015;45:2263-75 pubmed publisher
	Ranjan K, Hedl M, Sinha S, Zhang X, Abraham C. Ubiquitination of ATF6 by disease-associated RNF186 promotes the innate receptor-induced unfolded protein response. J Clin Invest. 2021;131: pubmed publisher
	Wang N, Yi H, Fang L, Jin J, Ma Q, Shen Y, et al. CD226 Attenuates Treg Proliferation via Akt and Erk Signaling in an EAE Model. Front Immunol. 2020;11:1883 pubmed publisher
	Xiao S, Bod L, Pochet N, KOTA S, Hu D, Madi A, et al. Checkpoint Receptor TIGIT Expressed on Tim-1+ B Cells Regulates Tissue Inflammation. Cell Rep. 2020;32:107892 pubmed publisher
	Andersen L, Gülich A, Alteneder M, Preglej T, Orola M, Dhele N, et al. The Transcription Factor MAZR/PATZ1 Regulates the Development of FOXP3+ Regulatory T Cells. Cell Rep. 2019;29:4447-4459.e6 pubmed publisher
	Piper C, Rosser E, Oleinika K, Nistala K, Krausgruber T, Rendeiro A, et al. Aryl Hydrocarbon Receptor Contributes to the Transcriptional Program of IL-10-Producing Regulatory B Cells. Cell Rep. 2019;29:1878-1892.e7 pubmed publisher
	Thomas A, Dong Y, Beskid N, Garcia A, Adams A, Babensee J. Brief exposure to hyperglycemia activates dendritic cells in vitro and in vivo. J Cell Physiol. 2020;235:5120-5129 pubmed publisher
	Campos Mora M, Contreras Kallens P, Gálvez Jirón F, Rojas M, Rojas C, Refisch A, et al. CD4+Foxp3+T Regulatory Cells Promote Transplantation Tolerance by Modulating Effector CD4+ T Cells in a Neuropilin-1-Dependent Manner. Front Immunol. 2019;10:882 pubmed publisher
	Sun R, Hedl M, Abraham C. Twist1 and Twist2 Induce Human Macrophage Memory upon Chronic Innate Receptor Treatment by HDAC-Mediated Deacetylation of Cytokine Promoters. J Immunol. 2019;202:3297-3308 pubmed publisher
	Min C, Kim H, Ha N, Kim Y, Kwon E, Yen N, et al. A Type I Interferon and IL-10 Induced by Orientia tsutsugamushi Infection Suppresses Antigen-Specific T Cells and Their Memory Responses. Front Immunol. 2018;9:2022 pubmed publisher
	He L, Zhou S, Qi Q, Chi Y, Zhu J, Xu Z, et al. The regulation of regulation: interleukin-10 increases CD4+ CD25+ regulatory T cells but impairs their immunosuppressive activity in murine models with schistosomiasis japonica or asthma. Immunology. 2018;153:84-96 pubmed publisher
	Brinkman C, Iwami D, Hritzo M, Xiong Y, Ahmad S, Simon T, et al. Treg engage lymphotoxin beta receptor for afferent lymphatic transendothelial migration. Nat Commun. 2016;7:12021 pubmed publisher
	Xu G, Liu D, Fan Y, Yang X, Korner H, Fu Y, et al. Lymphotoxin alpha beta 2 (membrane lymphotoxin) is critically important for resistance to Leishmania major infection in mice. J Immunol. 2007;179:5358-66 pubmed
	Riemann M, Endres R, Liptay S, Pfeffer K, Schmid R. The IkappaB protein Bcl-3 negatively regulates transcription of the IL-10 gene in macrophages. J Immunol. 2005;175:3560-8 pubmed

product information

Antigen :

IL-10

Apps. Abbrev. :

ELISA Capture, Western Blotting

Cat # :

504901

Clone :

JES5-2A5

Item :

Purified anti-mouse IL-10

Isotype :

Rat IgG1, κ

Other Names :

Interleukin-10, Cytokine synthesis inhibitory factor (CSIF), B cell derived T cell growth factor (B-TCGF), T cell growth inhibitory factor (TGIF)

Size :

50 μg

Price (USD) :

83 USD

Reactivity :

Mouse

Clonality :

Monoclonal

Host :

Rat

Conjugate/Tag/Label :

PURE

Immunogen :

E. coli -expressed, recombinant mouse IL-10

Application Notes :

ELISA or ELISPOT Capture 1-5,7,10 : The purified JES5-2A5 antibody is useful as the capture antibody in a sandwich ELISA or ELISPOT assay, when used in conjunction with the biotinylated JES5-16E3 antibody (Cat. No. 505004) as the detecting antibody. The LEAF™ purified antibody is suggested for ELISPOT capture. ELISA or ELISPOT Detection: The biotinylated JES5-2A5 antibody is useful as the detecting antibody in a sandwich ELISA or ELISPOT assay, when used in conjunction with the Purified JES5-16E3 antibody (Cat. No. 505002/505012) as the capture antibody. Neutralization 2,8,9 : The LEAF™ purified antibody (Endotoxin 0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for neutralization of mouse IL-10 bioactivity in vivo and in vitro (Cat. No. 504904). Additional reported applications (for the relevant formats) include: Western blotting 12 and in vivo capture 6 . Note: For testing mouse IL-10 in serum, plasma or supernatant, BioLegend s ELISA Max™ Sets (Cat. No. 431401 to 431406) are specially developed and recommended.

more info or order :

BioLegend product webpage