Ultra-LEAF™ Purified anti-mouse IL-6 | BioLegend 504516 product information

product summary

company name :

BioLegend

other brands :

Babco, Signet, Sternberger Monoclonals, Senetek, Covance

product type :

antibody

product name :

Ultra-LEAF™ Purified anti-mouse IL-6

catalog :

504516

quantity :

100 mg

price :

3383 USD

clonality :

monoclonal

host :

rat

conjugate :

nonconjugated

clone name :

MP5-20F3

reactivity :

mouse

application :

ELISA, neutralization, flow cytometry, immunohistochemistry - frozen section

more info or order :

BioLegend product webpage

citations: 4

Published Application/Species/Sample/Dilution	Reference
flow cytometry; mouse; loading ...; fig 4c, 4d	Meryk A, Grasse M, Balasco L, Kapferer W, Grubeck Loebenstein B, Pangrazzi L. Antioxidants N-Acetylcysteine and Vitamin C Improve T Cell Commitment to Memory and Long-Term Maintenance of Immunological Memory in Old Mice. Antioxidants (Basel). 2020;9: pubmed publisher
flow cytometry; mouse; loading ...; fig 2d	Forbester J, Clement M, Wellington D, Yeung A, Dimonte S, Marsden M, et al. IRF5 Promotes Influenza Virus-Induced Inflammatory Responses in Human Induced Pluripotent Stem Cell-Derived Myeloid Cells and Murine Models. J Virol. 2020;94: pubmed publisher
	Izumi G, Nakano H, Nakano K, Whitehead G, Grimm S, Fessler M, et al. CD11b+ lung dendritic cells at different stages of maturation induce Th17 or Th2 differentiation. Nat Commun. 2021;12:5029 pubmed publisher
	Pasciuto E, Burton O, Roca C, Lagou V, Rajan W, Theys T, et al. Microglia Require CD4 T Cells to Complete the Fetal-to-Adult Transition. Cell. 2020;182:625-640.e24 pubmed publisher

product information

Antigen :

IL-6

Apps. Abbrev. :

ELISA Capture, CyTOF®, ELISPOT Capture, Neut, IHC-F

Cat # :

504516

Clone :

MP5-20F3

Item :

Ultra-LEAF™ Purified anti-mouse IL-6

Isotype :

Rat IgG1, κ

Other Names :

Interleukin-6, B cell stimulating factor-2 (BSF-2), Cytotoxic T cell differentiation factor (CDF), Hepatocyte stimulating factor (HSF), Hybridoma/plasmacytoma growth factor (HPGF)

Size :

100 mg

Price (USD) :

3383 USD

Reactivity :

Mouse

Clonality :

Monoclonal

Host :

Rat

Conjugate/Tag/Label :

ULEAF

Immunogen :

COS-7-expressed, recombinant mouse IL-6

Application Notes :

ELISA Capture 1-3,11 or ELISPOT Capture 4,5 : The purified MP5-20F3 antibody is useful as the capture antibody in a sandwich ELISA or ELISPOT assay, when used in conjunction with the biotinylated MP5-32C11 antibody (Cat. No. 504602) as the detecting antibody and recombinant mouse IL-6 (Cat. No. 575709) as the standard. The Ultra-LEAF purified antibody is suggested for ELISPOT capture. Flow Cytometry: The fluorochrome-labeled MP5-20F3 antibody is useful for intracellular immunofluorescent staining and flow cytometric analysis to identify IL-6 -producing cells within mixed cell populations. For intracellular cytokine staining protocol, please visit www.biolegend.com and click on the support section. Neutralization 1-2,8-10 : The Ultra-LEAF purified antibody (Endotoxin 0.1 EU/ug, Azide-Free, 0.2 um filtered) is recommended for neutralization of mouse IL-6 bioactivity in vivo and in vitro (Cat. Nos. 504511-504516). Additional reported applications (for relevant formats of this clone) include: In Vivo Capture 7 , and immunohistochemical staining (paraformaldehyde-fixed/saponin-treated 6 , and acetone-fixed frozen tissue sections 13, 14 ). Note: For testing mouse IL-6 in serum, plasma or supernatant, BioLegend s ELISA Max Sets (Cat. Nos. 431301 to 431306) are specially developed and recommended.

more info or order :

BioLegend product webpage