APC anti-mouse IL-6 | BioLegend 504507 product information

product summary

company name :

BioLegend

other brands :

Babco, Signet, Sternberger Monoclonals, Senetek, Covance

product type :

antibody

product name :

APC anti-mouse IL-6

catalog :

504507

quantity :

25 μg

price :

102 USD

clonality :

monoclonal

host :

rat

conjugate :

APC

clone name :

MP5-20F3

reactivity :

mouse

application :

flow cytometry

more info or order :

BioLegend product webpage

citations: 9

Reference
Darzianiazizi M, Mehrani Y, Chan L, Mould R, Kulkarni R, Sharif S, et al. Type I Interferon α/β Receptor-Mediated Signaling Negatively Regulates Antiviral Cytokine Responses in Murine Bone-Marrow-Derived Mast Cells and Protects the Cells from Virus-Induced Cell Death. Int J Mol Sci. 2020;21: pubmed publisher
Orozco S, Daniels B, Yatim N, Messmer M, Quarato G, Chen Harris H, et al. RIPK3 Activation Leads to Cytokine Synthesis that Continues after Loss of Cell Membrane Integrity. Cell Rep. 2019;28:2275-2287.e5 pubmed publisher
Matsumura T, Ikebe T, Arikawa K, Hosokawa M, Aiko M, Iguchi A, et al. Sequential Sensing by TLR2 and Mincle Directs Immature Myeloid Cells to Protect against Invasive Group A Streptococcal Infection in Mice. Cell Rep. 2019;27:561-571.e6 pubmed publisher
Farsakoglu Y, Palomino Segura M, Latino I, Zanaga S, Chatziandreou N, Pizzagalli D, et al. Influenza Vaccination Induces NK-Cell-Mediated Type-II IFN Response that Regulates Humoral Immunity in an IL-6-Dependent Manner. Cell Rep. 2019;26:2307-2315.e5 pubmed publisher
Martínez López M, Iborra S, Conde Garrosa R, Mastrangelo A, Danne C, Mann E, et al. Microbiota Sensing by Mincle-Syk Axis in Dendritic Cells Regulates Interleukin-17 and -22 Production and Promotes Intestinal Barrier Integrity. Immunity. 2019;50:446-461.e9 pubmed publisher
Philip N, DeLaney A, Peterson L, Santos Marrero M, Grier J, Sun Y, et al. Activity of Uncleaved Caspase-8 Controls Anti-bacterial Immune Defense and TLR-Induced Cytokine Production Independent of Cell Death. PLoS Pathog. 2016;12:e1005910 pubmed publisher
Paranjape A, Chernushevich O, Qayum A, Spence A, Taruselli M, Abebayehu D, et al. Dexamethasone rapidly suppresses IL-33-stimulated mast cell function by blocking transcription factor activity. J Leukoc Biol. 2016;100:1395-1404 pubmed
Colliou N, Sahay B, Zadeh M, Owen J, Mohamadzadeh M. Impact of Gastrointestinal Bacillus anthracis Infection on Hepatic B Cells. Toxins (Basel). 2015;7:3805-17 pubmed publisher
Copenhaver A, Casson C, Nguyen H, Duda M, Shin S. IL-1R signaling enables bystander cells to overcome bacterial blockade of host protein synthesis. Proc Natl Acad Sci U S A. 2015;112:7557-62 pubmed publisher

product information

Antigen :

IL-6

Apps. Abbrev. :

Intracellular Staining for Flow Cytometry

Cat # :

504507

Clone :

MP5-20F3

Item :

APC anti-mouse IL-6

Isotype :

Rat IgG1, κ

Other Names :

Interleukin-6, B cell stimulating factor-2 (BSF-2), Cytotoxic T cell differentiation factor (CDF), Hepatocyte stimulating factor (HSF), Hybridoma/plasmacytoma growth factor (HPGF)

Size :

25 μg

Price (USD) :

102 USD

Reactivity :

Mouse

Clonality :

Monoclonal

Host :

Rat

Conjugate/Tag/Label :

APC

Immunogen :

COS-7-expressed, recombinant mouse IL-6

Application Notes :

ELISA Capture 1-3,11 or ELISPOT Capture 4,5 : The purified MP5-20F3 antibody is useful as the capture antibody in a sandwich ELISA or ELISPOT assay, when used in conjunction with the biotinylated MP5-32C11 antibody (Cat. No. 504602) as the detecting antibody and recombinant mouse IL-6 (Cat. No. 575709) as the standard. The Ultra-LEAF purified antibody is suggested for ELISPOT capture. Flow Cytometry: The fluorochrome-labeled MP5-20F3 antibody is useful for intracellular immunofluorescent staining and flow cytometric analysis to identify IL-6 -producing cells within mixed cell populations. For intracellular cytokine staining protocol, please visit www.biolegend.com and click on the support section. Neutralization 1-2,8-10 : The Ultra-LEAF purified antibody (Endotoxin 0.1 EU/ug, Azide-Free, 0.2 um filtered) is recommended for neutralization of mouse IL-6 bioactivity in vivo and in vitro (Cat. No. 504506). Additional reported applications (for relevant formats of this clone) include: In Vivo Capture 7 , and immunohistochemical staining (paraformaldehyde-fixed/saponin-treated 6 , and acetone-fixed frozen tissue sections 13, 14 ). Note: For testing mouse IL-6 in serum, plasma or supernatant, BioLegend s ELISA Max Sets (Cat. No. 431301 to 431306) are specially developed and recommended.

more info or order :

BioLegend product webpage