PE anti-mouse IL-6 | BioLegend 504503 product information

product summary

company name :

BioLegend

other brands :

Babco, Signet, Sternberger Monoclonals, Senetek, Covance

product type :

antibody

product name :

PE anti-mouse IL-6

catalog :

504503

quantity :

25 μg

price :

96 USD

clonality :

monoclonal

host :

rat

conjugate :

clone name :

MP5-20F3

reactivity :

mouse

application :

flow cytometry

more info or order :

BioLegend product webpage

citations: 17

Published Application/Species/Sample/Dilution	Reference
flow cytometry; mouse; loading ...; fig 2d	Forbester J, Clement M, Wellington D, Yeung A, Dimonte S, Marsden M, et al. IRF5 Promotes Influenza Virus-Induced Inflammatory Responses in Human Induced Pluripotent Stem Cell-Derived Myeloid Cells and Murine Models. J Virol. 2020;94: pubmed publisher
flow cytometry; mouse; loading ...; fig 3d	Yan D, Wang J, Sun H, Zamani A, Zhang H, Chen W, et al. TIPE2 specifies the functional polarization of myeloid-derived suppressor cells during tumorigenesis. J Exp Med. 2020;217: pubmed publisher
flow cytometry; mouse; 1:100; loading ...; fig 7A	Li C, Bi Y, Li Y, Yang H, Yu Q, Wang J, et al. Dendritic cell MST1 inhibits Th17 differentiation. Nat Commun. 2017;8:14275 pubmed publisher
flow cytometry; mouse	OlguÃn J, FernÃ¡ndez J, Salinas N, JuÃ¡rez I, Rodriguez Sosa M, Campuzano J, et al. Adoptive transfer of CD4(+)Foxp3(+) regulatory T cells to C57BL/6J mice during acute infection with Toxoplasma gondii down modulates the exacerbated Th1 immune response. Microbes Infect. 2015;17:586-95 pubmed publisher
	Wright J, Bazile C, Clark E, Carlesso G, Boucher J, Kleiman E, et al. Impaired B Cell Apoptosis Results in Autoimmunity That Is Alleviated by Ablation of Btk. Front Immunol. 2021;12:705307 pubmed publisher
	Castoldi A, Monteiro L, van Teijlingen Bakker N, Sanin D, Rana N, Corrado M, et al. Triacylglycerol synthesis enhances macrophage inflammatory function. Nat Commun. 2020;11:4107 pubmed publisher
	Rosser E, Piper C, Matei D, Blair P, Rendeiro A, Orford M, et al. Microbiota-Derived Metabolites Suppress Arthritis by Amplifying Aryl-Hydrocarbon Receptor Activation in Regulatory B Cells. Cell Metab. 2020;31:837-851.e10 pubmed publisher
	Mogilenko D, Haas J, L homme L, Fleury S, Quemener S, Levavasseur M, et al. Metabolic and Innate Immune Cues Merge into a Specific Inflammatory Response via the UPR. Cell. 2019;177:1201-1216.e19 pubmed publisher
	Piliponsky A, Shubin N, Lahiri A, Truong P, Clauson M, Niino K, et al. Basophil-derived tumor necrosis factor can enhance survival in a sepsis model in mice. Nat Immunol. 2019;20:129-140 pubmed publisher
	Lyons D, Plewes M, Pullen N. Soluble transforming growth factor beta-1 enhances murine mast cell release of Interleukin 6 in IgE-independent and Interleukin 13 in IgE-dependent settings in vitro. PLoS ONE. 2018;13:e0207704 pubmed publisher
	Cohen M, Giladi A, Gorki A, Solodkin D, Zada M, Hladik A, et al. Lung Single-Cell Signaling Interaction Map Reveals Basophil Role in Macrophage Imprinting. Cell. 2018;175:1031-1044.e18 pubmed publisher
	Prystaz K, Kaiser K, Kovtun A, Haffner Luntzer M, Fischer V, Rapp A, et al. Distinct Effects of IL-6 Classic and Trans-Signaling in Bone Fracture Healing. Am J Pathol. 2018;188:474-490 pubmed publisher
	Zhu M, Zou J, Li T, O Brien S, Zhang Y, Ogden S, et al. Differential Roles of Phospholipase D Proteins in FcÎµRI-Mediated Signaling and Mast Cell Function. J Immunol. 2015;195:4492-502 pubmed publisher
	Yu C, Micaroni M, Puyskens A, Schultz T, Yeo J, Stanley A, et al. RP105 Engages Phosphatidylinositol 3-Kinase p110Î´ To Facilitate the Trafficking and Secretion of Cytokines in Macrophages during Mycobacterial Infection. J Immunol. 2015;195:3890-900 pubmed publisher
	Meher A, Johnston W, Lu G, Pope N, Bhamidipati C, Harmon D, et al. B2 cells suppress experimental abdominal aortic aneurysms. Am J Pathol. 2014;184:3130-41 pubmed publisher
	Shiomi A, Usui T, Ishikawa Y, Shimizu M, Murakami K, Mimori T. GM-CSF but not IL-17 is critical for the development of severe interstitial lung disease in SKG mice. J Immunol. 2014;193:849-59 pubmed publisher
	Kuwahara M, Suzuki J, Tofukuji S, Yamada T, Kanoh M, Matsumoto A, et al. The Menin-Bach2 axis is critical for regulating CD4 T-cell senescence and cytokine homeostasis. Nat Commun. 2014;5:3555 pubmed publisher

product information

Antigen :

IL-6

Apps. Abbrev. :

Intracellular Staining for Flow Cytometry

Cat # :

504503

Clone :

MP5-20F3

Item :

PE anti-mouse IL-6

Isotype :

Rat IgG1, κ

Other Names :

Interleukin-6, B cell stimulating factor-2 (BSF-2), Cytotoxic T cell differentiation factor (CDF), Hepatocyte stimulating factor (HSF), Hybridoma/plasmacytoma growth factor (HPGF)

Size :

25 μg

Price (USD) :

96 USD

Reactivity :

Mouse

Clonality :

Monoclonal

Host :

Rat

Conjugate/Tag/Label :

Immunogen :

COS-7-expressed, recombinant mouse IL-6

Application Notes :

ELISA Capture 1-3,11 or ELISPOT Capture 4,5 : The purified MP5-20F3 antibody is useful as the capture antibody in a sandwich ELISA or ELISPOT assay, when used in conjunction with the biotinylated MP5-32C11 antibody (Cat. No. 504602) as the detecting antibody and recombinant mouse IL-6 (Cat. No. 575709) as the standard. The Ultra-LEAF purified antibody is suggested for ELISPOT capture. Flow Cytometry: The fluorochrome-labeled MP5-20F3 antibody is useful for intracellular immunofluorescent staining and flow cytometric analysis to identify IL-6 -producing cells within mixed cell populations. For intracellular cytokine staining protocol, please visit www.biolegend.com and click on the support section. Neutralization 1-2,8-10 : The Ultra-LEAF purified antibody (Endotoxin 0.1 EU/ug, Azide-Free, 0.2 um filtered) is recommended for neutralization of mouse IL-6 bioactivity in vivo and in vitro (Cat. No. 504506). Additional reported applications (for relevant formats of this clone) include: In Vivo Capture 7 , and immunohistochemical staining (paraformaldehyde-fixed/saponin-treated 6 , and acetone-fixed frozen tissue sections 13, 14 ). Note: For testing mouse IL-6 in serum, plasma or supernatant, BioLegend s ELISA Max Sets (Cat. No. 431301 to 431306) are specially developed and recommended.

more info or order :

BioLegend product webpage