Purified anti-mouse IL-6 | BioLegend 504501 product information

product summary

company name :

BioLegend

other brands :

Babco, Signet, Sternberger Monoclonals, Senetek, Covance

product type :

antibody

product name :

Purified anti-mouse IL-6

catalog :

504501

quantity :

50 μg

price :

83 USD

clonality :

monoclonal

host :

rat

conjugate :

nonconjugated

clone name :

MP5-20F3

reactivity :

mouse

application :

ELISA, neutralization, flow cytometry, immunohistochemistry - frozen section

more info or order :

BioLegend product webpage

citations: 27

Published Application/Species/Sample/Dilution	Reference
flow cytometry; mouse; loading ...; fig 4c, 4d	Meryk A, Grasse M, Balasco L, Kapferer W, Grubeck Loebenstein B, Pangrazzi L. Antioxidants N-Acetylcysteine and Vitamin C Improve T Cell Commitment to Memory and Long-Term Maintenance of Immunological Memory in Old Mice. Antioxidants (Basel). 2020;9: pubmed publisher
flow cytometry; mouse; loading ...; fig 2d	Forbester J, Clement M, Wellington D, Yeung A, Dimonte S, Marsden M, et al. IRF5 Promotes Influenza Virus-Induced Inflammatory Responses in Human Induced Pluripotent Stem Cell-Derived Myeloid Cells and Murine Models. J Virol. 2020;94: pubmed publisher
flow cytometry; mouse; loading ...; fig 4c	Stacey M, Clare S, Clement M, Marsden M, Abdul Karim J, Kane L, et al. The antiviral restriction factor IFN-induced transmembrane protein 3 prevents cytokine-driven CMV pathogenesis. J Clin Invest. 2017;127:1463-1474 pubmed publisher
flow cytometry; mouse; 1:100; loading ...; fig 7A	Li C, Bi Y, Li Y, Yang H, Yu Q, Wang J, et al. Dendritic cell MST1 inhibits Th17 differentiation. Nat Commun. 2017;8:14275 pubmed publisher
flow cytometry; mouse; fig 5	LasigliÃ¨ D, Boero S, Bauer I, Morando S, Damonte P, Cea M, et al. Sirt6 regulates dendritic cell differentiation, maturation, and function. Aging (Albany NY). 2016;8:34-49 pubmed
ELISA; mouse; fig 5	Lee J, Park K, Han D, Bang N, Kim D, Na H, et al. PharmDB-K: Integrated Bio-Pharmacological Network Database for Traditional Korean Medicine. PLoS ONE. 2015;10:e0142624 pubmed publisher
	Rosser E, Piper C, Matei D, Blair P, Rendeiro A, Orford M, et al. Microbiota-Derived Metabolites Suppress Arthritis by Amplifying Aryl-Hydrocarbon Receptor Activation in Regulatory B Cells. Cell Metab. 2020;31:837-851.e10 pubmed publisher
	Wu G, Huang F, Chen Y, Zhuang Y, Huang Y, Xie Y. High Levels of BMP2 Promote Liver Cancer Growth via the Activation of Myeloid-Derived Suppressor Cells. Front Oncol. 2020;10:194 pubmed publisher
	Schwaderer J, Phan T, Glöckner A, Delp J, Leist M, Brunner T, et al. Pharmacological LRH-1/Nr5a2 inhibition limits pro-inflammatory cytokine production in macrophages and associated experimental hepatitis. Cell Death Dis. 2020;11:154 pubmed publisher
	Ji Z, Wu S, Xu Y, Qi J, Su X, Shen L. Obesity Promotes EAE Through IL-6 and CCL-2-Mediated T Cells Infiltration. Front Immunol. 2019;10:1881 pubmed publisher
	Sun R, Hedl M, Abraham C. Twist1 and Twist2 Induce Human Macrophage Memory upon Chronic Innate Receptor Treatment by HDAC-Mediated Deacetylation of Cytokine Promoters. J Immunol. 2019;202:3297-3308 pubmed publisher
	Malara A, Gruppi C, Abbonante V, Cattaneo D, De Marco L, Massa M, et al. EDA fibronectin-TLR4 axis sustains megakaryocyte expansion and inflammation in bone marrow fibrosis. J Exp Med. 2019;216:587-604 pubmed publisher
	Rosen D, Seki S, Fernandez Castaneda A, Beiter R, Eccles J, Woodfolk J, et al. Modulation of the sigma-1 receptor-IRE1 pathway is beneficial in preclinical models of inflammation and sepsis. Sci Transl Med. 2019;11: pubmed publisher
	Al Sayed M, Amrein M, Bührer E, Huguenin A, Radpour R, Riether C, et al. T-cell-Secreted TNFα Induces Emergency Myelopoiesis and Myeloid-Derived Suppressor Cell Differentiation in Cancer. Cancer Res. 2019;79:346-359 pubmed publisher
	Tanaka S, Suto A, Iwamoto T, Kageyama T, Tamachi T, Takatori H, et al. Sox12 promotes T reg differentiation in the periphery during colitis. J Exp Med. 2018;215:2509-2519 pubmed publisher
	Crawford G, Hayes M, Seoane R, Ward S, Dalessandri T, Lai C, et al. Epithelial damage and tissue γδ T cells promote a unique tumor-protective IgE response. Nat Immunol. 2018;19:859-870 pubmed publisher
	Xu M, Lu H, Lee Y, Wu Y, Liu K, Shi Y, et al. An Interleukin-25-Mediated Autoregulatory Circuit in Keratinocytes Plays a Pivotal Role in Psoriatic Skin Inflammation. Immunity. 2018;48:787-798.e4 pubmed publisher
	Pushalkar S, Hundeyin M, Daley D, Zambirinis C, Kurz E, Mishra A, et al. The Pancreatic Cancer Microbiome Promotes Oncogenesis by Induction of Innate and Adaptive Immune Suppression. Cancer Discov. 2018;8:403-416 pubmed publisher
	St Leger A, Hansen A, Karauzum H, Horai R, Yu C, Laurence A, et al. STAT-3-independent production of IL-17 by mouse innate-like αβ T cells controls ocular infection. J Exp Med. 2018;215:1079-1090 pubmed publisher
	Meier L, Auger J, Engelson B, Cowan H, Breed E, Gonzalez Torres M, et al. CD301b/MGL2+ Mononuclear Phagocytes Orchestrate Autoimmune Cardiac Valve Inflammation and Fibrosis. Circulation. 2018;137:2478-2493 pubmed publisher
	Li B, Wang X, Choi I, Wang Y, Liu S, Pham A, et al. miR-146a modulates autoreactive Th17 cell differentiation and regulates organ-specific autoimmunity. J Clin Invest. 2017;127:3702-3716 pubmed publisher
	Peterson L, Philip N, DeLaney A, Wynosky Dolfi M, Asklof K, Gray F, et al. RIPK1-dependent apoptosis bypasses pathogen blockade of innate signaling to promote immune defense. J Exp Med. 2017;214:3171-3182 pubmed publisher
	Burbage M, Keppler S, Montaner B, Mattila P, Batista F. The Small Rho GTPase TC10 Modulates B Cell Immune Responses. J Immunol. 2017;199:1682-1695 pubmed publisher
	Song K, Xu L, Zhang W, Cai Y, Jang B, Oh J, et al. Laminarin promotes anti-cancer immunity by the maturation of dendritic cells. Oncotarget. 2017;8:38554-38567 pubmed publisher
	Daley D, Mani V, Mohan N, Akkad N, Ochi A, Heindel D, et al. Dectin 1 activation on macrophages by galectin 9 promotes pancreatic carcinoma and peritumoral immune tolerance. Nat Med. 2017;23:556-567 pubmed publisher
	Ohlund D, Handly Santana A, Biffi G, Elyada E, Almeida A, Ponz Sarvisé M, et al. Distinct populations of inflammatory fibroblasts and myofibroblasts in pancreatic cancer. J Exp Med. 2017;214:579-596 pubmed publisher
	Ozeki Y, Sugawara I, Udagawa T, Aoki T, Osada Oka M, Tateishi Y, et al. Transient role of CD4+CD25+ regulatory T cells in mycobacterial infection in mice. Int Immunol. 2010;22:179-89 pubmed publisher

product information

Antigen :

IL-6

Apps. Abbrev. :

ELISA Capture, CyTOF®, ELISPOT Capture, Neut, IHC-F

Cat # :

504501

Clone :

MP5-20F3

Item :

Purified anti-mouse IL-6

Isotype :

Rat IgG1, κ

Other Names :

Interleukin-6, B cell stimulating factor-2 (BSF-2), Cytotoxic T cell differentiation factor (CDF), Hepatocyte stimulating factor (HSF), Hybridoma/plasmacytoma growth factor (HPGF)

Size :

50 μg

Price (USD) :

83 USD

Reactivity :

Mouse

Clonality :

Monoclonal

Host :

Rat

Conjugate/Tag/Label :

PURE

Immunogen :

COS-7-expressed, recombinant mouse IL-6

Application Notes :

ELISA Capture 1-3,11 or ELISPOT Capture 4,5 : The purified MP5-20F3 antibody is useful as the capture antibody in a sandwich ELISA or ELISPOT assay, when used in conjunction with the biotinylated MP5-32C11 antibody (Cat. No. 504602) as the detecting antibody and recombinant mouse IL-6 (Cat. No. 575709) as the standard. The Ultra-LEAF purified antibody is suggested for ELISPOT capture. Flow Cytometry: The fluorochrome-labeled MP5-20F3 antibody is useful for intracellular immunofluorescent staining and flow cytometric analysis to identify IL-6 -producing cells within mixed cell populations. For intracellular cytokine staining protocol, please visit www.biolegend.com and click on the support section. Neutralization 1-2,8-10 : The Ultra-LEAF purified antibody (Endotoxin 0.1 EU/ug, Azide-Free, 0.2 um filtered) is recommended for neutralization of mouse IL-6 bioactivity in vivo and in vitro (Cat. No. 504506). Additional reported applications (for relevant formats of this clone) include: In Vivo Capture 7 , and immunohistochemical staining (paraformaldehyde-fixed/saponin-treated 6 , and acetone-fixed frozen tissue sections 13, 14 ). Note: For testing mouse IL-6 in serum, plasma or supernatant, BioLegend s ELISA Max Sets (Cat. No. 431301 to 431306) are specially developed and recommended.

more info or order :

BioLegend product webpage