Ultra-LEAF™ Purified anti-mouse/human IL-5 | BioLegend 504317 product information

product summary

company name :

BioLegend

other brands :

Babco, Signet, Sternberger Monoclonals, Senetek, Covance

product type :

antibody

product name :

Ultra-LEAF™ Purified anti-mouse/human IL-5

catalog :

504317

quantity :

50 mg

price :

2354 USD

clonality :

monoclonal

host :

rat

conjugate :

nonconjugated

clone name :

TRFK5

reactivity :

human, mouse

application :

western blot, ELISA, immunohistochemistry, immunocytochemistry, neutralization, flow cytometry

more info or order :

BioLegend product webpage

citations: 10

Published Application/Species/Sample/Dilution	Reference
flow cytometry; mouse; loading ...; fig s5a	Liu Y, Du J, Liu X, Wang L, Han Y, Huang C, et al. MG149 inhibits histone acetyltransferase KAT8-mediated IL-33 acetylation to alleviate allergic airway inflammation and airway hyperresponsiveness. Signal Transduct Target Ther. 2021;6:321 pubmed publisher
flow cytometry; mouse; loading ...; fig 3b	Kong W, Tsuyama N, Inoue H, Guo Y, Mokuda S, Nobukiyo A, et al. Long-chain saturated fatty acids in breast milk are associated with the pathogenesis of atopic dermatitis via induction of inflammatory ILC3s. Sci Rep. 2021;11:13109 pubmed publisher
flow cytometry; mouse; loading ...	Yoon S, Song S, Shin J, Kang S, Kim H, You H. Protective Effects of Korean Herbal Remedy against Airway Inflammation in an Allergic Asthma by Suppressing Eosinophil Recruitment and Infiltration in Lung. Antioxidants (Basel). 2020;10: pubmed publisher
flow cytometry; mouse; loading ...; fig 4s2a	Hayes M, Ward S, Crawford G, Seoane R, Jackson W, Kipling D, et al. Inflammation-induced IgE promotes epithelial hyperplasia and tumour growth. elife. 2020;9: pubmed publisher
flow cytometry; mouse; loading ...; fig 4b	Mantani P, Dunér P, Ljungcrantz I, Nilsson J, Bjorkbacka H, Fredrikson G. ILC2 transfers to apolipoprotein E deficient mice reduce the lipid content of atherosclerotic lesions. BMC Immunol. 2019;20:47 pubmed publisher
flow cytometry; mouse; loading ...; fig 4d	Qian L, Bajana S, Georgescu C, Peng V, Wang H, Adrianto I, et al. Suppression of ILC2 differentiation from committed T cell precursors by E protein transcription factors. J Exp Med. 2019;216:884-899 pubmed publisher
	Donado C, Cao A, Simmons D, Croker B, Brennan P, Brenner M. A Two-Cell Model for IL-1β Release Mediated by Death-Receptor Signaling. Cell Rep. 2020;31:107466 pubmed publisher
	Poe C, Youngblood C, Hodge K, KEMP K. Treatment of established TH2 cells with 4μ8c, an inhibitor of IRE1α, blocks IL-5 but not IL-4 secretion. BMC Immunol. 2019;20:3 pubmed publisher
	Miller M, Shehat M, Alcedo K, Spinel L, SOULAKOVA J, Tigno Aranjuez J. Frontline Science: RIP2 promotes house dust mite-induced allergic airway inflammation. J Leukoc Biol. 2018;104:447-459 pubmed publisher
	Meyer zu Horste G, Przybylski D, Schramm M, Wang C, Schnell A, Lee Y, et al. Fas Promotes T Helper 17 Cell Differentiation and Inhibits T Helper 1 Cell Development by Binding and Sequestering Transcription Factor STAT1. Immunity. 2018;48:556-569.e7 pubmed publisher

product information

Antigen :

IL-5

Apps. Abbrev. :

ELISA Capture, CyTOF®, ELISPOT Capture, Neut, ICFC, IHC, WB

Cat # :

504317

Clone :

TRFK5

Item :

Ultra-LEAF™ Purified anti-mouse/human IL-5

Isotype :

Rat IgG1, κ

Other Names :

Interleukin-5, Eosinophil differentiation factor (EDF), Eosinophil colony stimulating factor (Eo-CSF), B cell growth factor-2 (BCGF-2), B cell differentiation factor for IgM (BCDF-m), IgA enhancing factor (IgA-EF), T-cell replacing factor-1 (TRF-1)

Size :

50 mg

Price (USD) :

2354 USD

Reactivity :

Mouse, Human

Clonality :

Monoclonal

Host :

Rat

Conjugate/Tag/Label :

ULEAF

Immunogen :

Partially-purified T cell clone supernatant

Application Notes :

ELISA^1,3,4 or ELISPOT^2,6 Capture: The purified TRFK5 antibody is useful as a capture antibody for a sandwich ELISA or ELISPOT assay, when used in conjunction with biotinylated TRFK4 antibody (Cat. No. 504402) as the detection antibody for mouse IL-5, or used in conjunction with biotinylated JES1-5A10 antibody (Cat. No. 501002) as the detection antibody for human IL-5. The Ultra-LEAF™ purified antibody is suggested for ELISPOT capture.Flow Cytometry: The fluorochrome-labeled TRFK51 antibody is useful for intracellular immunofluorescent staining and flow cytometric analysis to identify IL-5-producing cells within mixed cell populations.Neutralization^1,9: The Ultra-LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for neutralization of human IL-5 bioactivity (Cat. Nos. 504313-504318).Additional reported applications (for the relevant formats) include: Western blotting, immunohistochemical staining of formalin-fixed paraffin-embedded tissue sections^8 and paraformaldehyde-fixed, saponin-treated frozen tissue sections^7, and immunocytochemistry.Note: For testing human IL-5 in serum or plasma, BioLegend's ELISA Max™ Sets (Cat. No. 430401 and 430404) are specially developed and recommended. For testing mouse IL-5 in serum, plasma or supernatant, BioLegend's ELISA MAX™ Sets (Cat. Nos. 431201 and 431206) are specially developed and recommended.;

more info or order :

BioLegend product webpage