Brilliant Violet 421™ anti-mouse/human IL-5 | BioLegend 504311 product information

product summary

company name :

BioLegend

other brands :

Babco, Signet, Sternberger Monoclonals, Senetek, Covance

product type :

antibody

product name :

Brilliant Violet 421™ anti-mouse/human IL-5

catalog :

504311

quantity :

50 μg

price :

253 USD

clonality :

monoclonal

host :

rat

conjugate :

BV421

clone name :

TRFK5

reactivity :

human, mouse

application :

flow cytometry

more info or order :

BioLegend product webpage

citations: 8

Reference
Golebski K, Layhadi J, Sahiner U, Steveling Klein E, Lenormand M, Li R, et al. Induction of IL-10-producing type 2 innate lymphoid cells by allergen immunotherapy is associated with clinical response. Immunity. 2021;54:291-307.e7 pubmed publisher
Yang R, Mele F, Worley L, Langlais D, Rosain J, Benhsaien I, et al. Human T-bet Governs Innate and Innate-like Adaptive IFN-γ Immunity against Mycobacteria. Cell. 2020;183:1826-1847.e31 pubmed publisher
Tuganbaev T, Mor U, Bashiardes S, Liwinski T, Nobs S, Leshem A, et al. Diet Diurnally Regulates Small Intestinal Microbiome-Epithelial-Immune Homeostasis and Enteritis. Cell. 2020;: pubmed publisher
Chinthrajah S, Cao S, Liu C, Lyu S, Sindher S, Long A, et al. Phase 2a randomized, placebo-controlled study of anti-IL-33 in peanut allergy. JCI Insight. 2019;4: pubmed publisher
Fu Z, Ye J, Dean J, Bostick J, Weinberg S, Xiong L, et al. Requirement of Mitochondrial Transcription Factor A in Tissue-Resident Regulatory T Cell Maintenance and Function. Cell Rep. 2019;28:159-171.e4 pubmed publisher
Golebski K, Ros X, Nagasawa M, van Tol S, Heesters B, Aglmous H, et al. IL-1β, IL-23, and TGF-β drive plasticity of human ILC2s towards IL-17-producing ILCs in nasal inflammation. Nat Commun. 2019;10:2162 pubmed publisher
Bachus H, Kaur K, Papillion A, Marquez Lago T, Yu Z, Ballesteros Tato A, et al. Impaired Tumor-Necrosis-Factor-α-driven Dendritic Cell Activation Limits Lipopolysaccharide-Induced Protection from Allergic Inflammation in Infants. Immunity. 2019;50:225-240.e4 pubmed publisher
Morimoto Y, Hirahara K, Kiuchi M, Wada T, Ichikawa T, Kanno T, et al. Amphiregulin-Producing Pathogenic Memory T Helper 2 Cells Instruct Eosinophils to Secrete Osteopontin and Facilitate Airway Fibrosis. Immunity. 2018;49:134-150.e6 pubmed publisher

product information

Antigen :

IL-5

Apps. Abbrev. :

Intracellular Staining for Flow Cytometry

Cat # :

504311

Clone :

TRFK5

Item :

Brilliant Violet 421™ anti-mouse/human IL-5

Isotype :

Rat IgG1, κ

Other Names :

Interleukin-5, Eosinophil differentiation factor (EDF), Eosinophil colony stimulating factor (Eo-CSF), B cell growth factor-2 (BCGF-2), B cell differentiation factor for IgM (BCDF-m), IgA enhancing factor (IgA-EF), T-cell replacing factor-1 (TRF-1)

Size :

50 μg

Price (USD) :

253 USD

Reactivity :

Mouse, Human

Clonality :

Monoclonal

Host :

Rat

Conjugate/Tag/Label :

BV421

Immunogen :

Partially-purified T cell clone supernatant

Application Notes :

ELISA 1,3,4 or ELISPOT 2,6 Capture: The purified TRFK5 antibody is useful as a capture antibody for a sandwich ELISA or ELISPOT assay, when used in conjunction with biotinylated TRFK4 antibody (Cat. No. 504402) as the detection antibody for mouse IL-5, or used in conjunction with biotinylated JES1-5A10 antibody (Cat. No. 501002) as the detection antibody for human IL-5. The Ultra-LEAF purified antibody is suggested for ELISPOT capture. Flow Cytometry: The fluorochrome-labeled TRFK51 antibody is useful for intracellular immunofluorescent staining and flow cytometric analysis to identify IL-5-producing cells within mixed cell populations. Neutralization 1,9 : The Ultra-LEAF purified antibody (Endotoxin 0.1 EU/ug, Azide-Free, 0.2 um filtered) is recommended for neutralization of human IL-5 bioactivity (Cat. Nos. 504313-504318). Additional reported applications (for the relevant formats) include: Western blotting, immunohistochemical staining of formalin-fixed paraffin-embedded tissue sections 8 and paraformaldehyde-fixed, saponin-treated frozen tissue sections 7 , and immunocytochemistry. Note: For testing human IL-5 in serum or plasma, BioLegend s ELISA Max Sets (Cat. No. 430401 and 430404) are specially developed and recommended. For testing mouse IL-5 in serum, plasma or supernatant, BioLegend s ELISA MAX Sets (Cat. Nos. 431201 and 431206) are specially developed and recommended.

more info or order :

BioLegend product webpage