APC anti-mouse/human IL-5 | BioLegend 504305 product information

product summary

company name :

BioLegend

other brands :

Babco, Signet, Sternberger Monoclonals, Senetek, Covance

product type :

antibody

product name :

APC anti-mouse/human IL-5

catalog :

504305

quantity :

25 μg

price :

102 USD

clonality :

monoclonal

host :

rat

conjugate :

APC

clone name :

TRFK5

reactivity :

human, mouse

application :

flow cytometry

more info or order :

BioLegend product webpage

citations: 24

Published Application/Species/Sample/Dilution	Reference
flow cytometry; mouse; loading ...; fig 4b	Mantani P, Dunér P, Ljungcrantz I, Nilsson J, Bjorkbacka H, Fredrikson G. ILC2 transfers to apolipoprotein E deficient mice reduce the lipid content of atherosclerotic lesions. BMC Immunol. 2019;20:47 pubmed publisher
flow cytometry; mouse; loading ...; fig 4d	Qian L, Bajana S, Georgescu C, Peng V, Wang H, Adrianto I, et al. Suppression of ILC2 differentiation from committed T cell precursors by E protein transcription factors. J Exp Med. 2019;216:884-899 pubmed publisher
	Bajana S, Pankow A, Liu K, Michniowska M, Urban J, Chen W, et al. Correlation between circulating innate lymphoid cell precursors and thymic function. iScience. 2022;25:103732 pubmed publisher
	Gu C, Upchurch K, Horton J, Wiest M, Zurawski S, Millard M, et al. Dectin-1 Controls TSLP-Induced Th2 Response by Regulating STAT3, STAT6, and p50-RelB Activities in Dendritic Cells. Front Immunol. 2021;12:678036 pubmed publisher
	Baldwin S, Reese V, Larsen S, Beebe E, Guderian J, Orr M, et al. Prophylactic efficacy against Mycobacterium tuberculosis using ID93 and lipid-based adjuvant formulations in the mouse model. PLoS ONE. 2021;16:e0247990 pubmed publisher
	Singh A, Kazer S, Roider J, Krista K, Millar J, Asowata O, et al. Innate Lymphoid Cell Activation and Sustained Depletion in Blood and Tissue of Children Infected with HIV from Birth Despite Antiretroviral Therapy. Cell Rep. 2020;32:108153 pubmed publisher
	Starkl P, Watzenboeck M, Popov L, Zahalka S, Hladik A, Lakovits K, et al. IgE Effector Mechanisms, in Concert with Mast Cells, Contribute to Acquired Host Defense against Staphylococcusaureus. Immunity. 2020;53:793-804.e9 pubmed publisher
	Corbett K, Edwards D, Leist S, Abiona O, Boyoglu Barnum S, Gillespie R, et al. SARS-CoV-2 mRNA vaccine design enabled by prototype pathogen preparedness. Nature. 2020;586:567-571 pubmed publisher
	He J, Yang Q, Xiao Q, Lei A, Li X, Zhou P, et al. IRF-7 Is a Critical Regulator of Type 2 Innate Lymphoid Cells in Allergic Airway Inflammation. Cell Rep. 2019;29:2718-2730.e6 pubmed publisher
	Shah R, Taccone M, Monaci E, Brito L, Bonci A, O Hagan D, et al. The droplet size of emulsion adjuvants has significant impact on their potency, due to differences in immune cell-recruitment and -activation. Sci Rep. 2019;9:11520 pubmed publisher
	Nagashima H, Mahlakõiv T, Shih H, Davis F, Meylan F, Huang Y, et al. Neuropeptide CGRP Limits Group 2 Innate Lymphoid Cell Responses and Constrains Type 2 Inflammation. Immunity. 2019;51:682-695.e6 pubmed publisher
	Timilshina M, You Z, Lacher S, Acharya S, Jiang L, Kang Y, et al. Activation of Mevalonate Pathway via LKB1 Is Essential for Stability of Treg Cells. Cell Rep. 2019;27:2948-2961.e7 pubmed publisher
	Walker J, Clark P, Crisp A, Barlow J, Szeto A, Ferreira A, et al. Polychromic Reporter Mice Reveal Unappreciated Innate Lymphoid Cell Progenitor Heterogeneity and Elusive ILC3 Progenitors in Bone Marrow. Immunity. 2019;: pubmed publisher
	Kim C, Lee C, Jung J, Ghosh A, Hasan S, Hwang S, et al. The Transcription Factor Ets1 Suppresses T Follicular Helper Type 2 Cell Differentiation to Halt the Onset of Systemic Lupus Erythematosus. Immunity. 2018;49:1034-1048.e8 pubmed publisher
	Shan M, Carrillo J, Yeste A, Gutzeit C, Segura Garzón D, Walland A, et al. Secreted IgD Amplifies Humoral T Helper 2 Cell Responses by Binding Basophils via Galectin-9 and CD44. Immunity. 2018;49:709-724.e8 pubmed publisher
	Li Q, Li D, Zhang X, Wan Q, Zhang W, Zheng M, et al. E3 Ligase VHL Promotes Group 2 Innate Lymphoid Cell Maturation and Function via Glycolysis Inhibition and Induction of Interleukin-33 Receptor. Immunity. 2018;48:258-270.e5 pubmed publisher
	Emgård J, Kammoun H, García Cassani B, Chesne J, Parigi S, Jacob J, et al. Oxysterol Sensing through the Receptor GPR183 Promotes the Lymphoid-Tissue-Inducing Function of Innate Lymphoid Cells and Colonic Inflammation. Immunity. 2018;48:120-132.e8 pubmed publisher
	Sun X, Tian Q, Wang L, Xue M, Zhong G. IL-6-mediated signaling pathways limit Chlamydia muridarum infection and exacerbate its pathogenicity in the mouse genital tract. Microbes Infect. 2017;19:536-545 pubmed publisher
	Schütze N, Trojandt S, Kuhn S, Tomm J, von Bergen M, Simon J, et al. Allergen-Induced IL-6 Regulates IL-9/IL-17A Balance in CD4+ T Cells in Allergic Airway Inflammation. J Immunol. 2016;197:2653-64 pubmed publisher
	Chen Z, Andreev D, Oeser K, Krljanac B, Hueber A, Kleyer A, et al. Th2 and eosinophil responses suppress inflammatory arthritis. Nat Commun. 2016;7:11596 pubmed publisher
	Schulze B, Piehler D, Eschke M, Heyen L, Protschka M, Kohler G, et al. Therapeutic expansion of CD4+FoxP3+ regulatory T cells limits allergic airway inflammation during pulmonary fungal infection. Pathog Dis. 2016;74:ftw020 pubmed publisher
	Colliou N, Sahay B, Zadeh M, Owen J, Mohamadzadeh M. Impact of Gastrointestinal Bacillus anthracis Infection on Hepatic B Cells. Toxins (Basel). 2015;7:3805-17 pubmed publisher
	Wiesner D, Specht C, Lee C, Smith K, Mukaremera L, Lee S, et al. Chitin recognition via chitotriosidase promotes pathologic type-2 helper T cell responses to cryptococcal infection. PLoS Pathog. 2015;11:e1004701 pubmed publisher
	Williams J, Yau D, Sethakorn N, Kach J, Reed E, Moore T, et al. RGS3 controls T lymphocyte migration in a model of Th2-mediated airway inflammation. Am J Physiol Lung Cell Mol Physiol. 2013;305:L693-701 pubmed publisher

product information

Antigen :

IL-5

Apps. Abbrev. :

Intracellular Staining for Flow Cytometry

Cat # :

504305

Clone :

TRFK5

Item :

APC anti-mouse/human IL-5

Isotype :

Rat IgG1, κ

Other Names :

Interleukin-5, Eosinophil differentiation factor (EDF), Eosinophil colony stimulating factor (Eo-CSF), B cell growth factor-2 (BCGF-2), B cell differentiation factor for IgM (BCDF-m), IgA enhancing factor (IgA-EF), T-cell replacing factor-1 (TRF-1)

Size :

25 μg

Price (USD) :

102 USD

Reactivity :

Mouse, Human

Clonality :

Monoclonal

Host :

Rat

Conjugate/Tag/Label :

APC

Immunogen :

Partially-purified T cell clone supernatant

Application Notes :

ELISA 1,3,4 or ELISPOT 2,6 Capture: The purified TRFK5 antibody is useful as a capture antibody for a sandwich ELISA or ELISPOT assay, when used in conjunction with biotinylated TRFK4 antibody (Cat. No. 504402) as the detection antibody for mouse IL-5, or used in conjunction with biotinylated JES1-5A10 antibody (Cat. No. 501002) as the detection antibody for human IL-5. The Ultra-LEAF purified antibody is suggested for ELISPOT capture. Flow Cytometry: The fluorochrome-labeled TRFK51 antibody is useful for intracellular immunofluorescent staining and flow cytometric analysis to identify IL-5-producing cells within mixed cell populations. Neutralization 1,9 : The Ultra-LEAF purified antibody (Endotoxin 0.1 EU/ug, Azide-Free, 0.2 um filtered) is recommended for neutralization of human IL-5 bioactivity (Cat. Nos. 504313-504318). Additional reported applications (for the relevant formats) include: Western blotting, immunohistochemical staining of formalin-fixed paraffin-embedded tissue sections 8 and paraformaldehyde-fixed, saponin-treated frozen tissue sections 7 , and immunocytochemistry. Note: For testing human IL-5 in serum or plasma, BioLegend s ELISA Max Sets (Cat. No. 430401 and 430404) are specially developed and recommended. For testing mouse IL-5 in serum, plasma or supernatant, BioLegend s ELISA MAX Sets (Cat. Nos. 431201 and 431206) are specially developed and recommended.

more info or order :

BioLegend product webpage