PE anti-mouse/human IL-5 | BioLegend 504303 product information

product summary

company name :

BioLegend

other brands :

Babco, Signet, Sternberger Monoclonals, Senetek, Covance

product type :

antibody

product name :

PE anti-mouse/human IL-5

catalog :

504303

quantity :

25 μg

price :

96 USD

clonality :

monoclonal

host :

rat

conjugate :

clone name :

TRFK5

reactivity :

human, mouse

application :

flow cytometry

more info or order :

BioLegend product webpage

citations: 20

Published Application/Species/Sample/Dilution	Reference
flow cytometry; mouse; loading ...; fig s5a	Liu Y, Du J, Liu X, Wang L, Han Y, Huang C, et al. MG149 inhibits histone acetyltransferase KAT8-mediated IL-33 acetylation to alleviate allergic airway inflammation and airway hyperresponsiveness. Signal Transduct Target Ther. 2021;6:321 pubmed publisher
flow cytometry; mouse; 1:100; tbl 1	Jackson Jones L, Duncan S, Magalhaes M, Campbell S, Maizels R, McSorley H, et al. Fat-associated lymphoid clusters control local IgM secretion during pleural infection and lung inflammation. Nat Commun. 2016;7:12651 pubmed publisher
flow cytometry; mouse; loading ...; fig 5a	Contreras F, Prado C, Gonzalez H, Franz D, Osorio Barrios F, Osorio F, et al. Dopamine Receptor D3 Signaling on CD4+ T Cells Favors Th1- and Th17-Mediated Immunity. J Immunol. 2016;196:4143-9 pubmed publisher
flow cytometry; mouse; loading ...; fig 3c	Smith R, Reyes N, Khandelwal P, Schlereth S, Lee H, Masli S, et al. Secondary allergic T cell responses are regulated by dendritic cell-derived thrombospondin-1 in the setting of allergic eye disease. J Leukoc Biol. 2016;100:371-80 pubmed publisher
	Baldwin S, Reese V, Larsen S, Beebe E, Guderian J, Orr M, et al. Prophylactic efficacy against Mycobacterium tuberculosis using ID93 and lipid-based adjuvant formulations in the mouse model. PLoS ONE. 2021;16:e0247990 pubmed publisher
	Huang X, Hurabielle C, Drummond R, Bouladoux N, Desai J, Sim C, et al. Murine model of colonization with fungal pathogen Candida auris to explore skin tropism, host risk factors and therapeutic strategies. Cell Host Microbe. 2021;29:210-221.e6 pubmed publisher
	Perner C, Flayer C, Zhu X, Aderhold P, Dewan Z, Voisin T, et al. Substance P Release by Sensory Neurons Triggers Dendritic Cell Migration and Initiates the Type-2 Immune Response to Allergens. Immunity. 2020;53:1063-1077.e7 pubmed publisher
	Li A, Xue M, Attia Z, Yu J, Lu M, Shan C, et al. Vesicular Stomatitis Virus and DNA Vaccines Expressing Zika Virus Nonstructural Protein 1 Induce Substantial but Not Sterilizing Protection against Zika Virus Infection. J Virol. 2020;94: pubmed publisher
	Vacca F, Chauch C, Jamwal A, Hinchy E, Heieis G, Webster H, et al. A helminth-derived suppressor of ST2 blocks allergic responses. elife. 2020;9: pubmed publisher
	Satoh Takayama N, Kato T, Motomura Y, Kageyama T, Taguchi Atarashi N, Kinoshita Daitoku R, et al. Bacteria-Induced Group 2 Innate Lymphoid Cells in the Stomach Provide Immune Protection through Induction of IgA. Immunity. 2020;52:635-649.e4 pubmed publisher
	Hurrell B, Galle Treger L, Jahani P, Howard E, Helou D, Banie H, et al. TNFR2 Signaling Enhances ILC2 Survival, Function, and Induction of Airway Hyperreactivity. Cell Rep. 2019;29:4509-4524.e5 pubmed publisher
	Galle Treger L, Hurrell B, Lewis G, Howard E, Jahani P, Banie H, et al. Autophagy is critical for group 2 innate lymphoid cell metabolic homeostasis and effector function. J Allergy Clin Immunol. 2020;145:502-517.e5 pubmed publisher
	Sasaki K, Himeno A, Nakagawa T, Sasaki Y, Kiyonari H, Iwai K. Modulation of autoimmune pathogenesis by T cell-triggered inflammatory cell death. Nat Commun. 2019;10:3878 pubmed publisher
	Galle Treger L, Sankaranarayanan I, Hurrell B, Howard E, Lo R, Maazi H, et al. Costimulation of type-2 innate lymphoid cells by GITR promotes effector function and ameliorates type 2 diabetes. Nat Commun. 2019;10:713 pubmed publisher
	Morimoto Y, Hirahara K, Kiuchi M, Wada T, Ichikawa T, Kanno T, et al. Amphiregulin-Producing Pathogenic Memory T Helper 2 Cells Instruct Eosinophils to Secrete Osteopontin and Facilitate Airway Fibrosis. Immunity. 2018;49:134-150.e6 pubmed publisher
	Hayatsu N, Miyao T, Tachibana M, Murakami R, Kimura A, Kato T, et al. Analyses of a Mutant Foxp3 Allele Reveal BATF as a Critical Transcription Factor in the Differentiation and Accumulation of Tissue Regulatory T Cells. Immunity. 2017;47:268-283.e9 pubmed publisher
	Amelio P, Portevin D, Reither K, Mhimbira F, Mpina M, Tumbo A, et al. Mixed Th1 and Th2 Mycobacterium tuberculosis-specific CD4 T cell responses in patients with active pulmonary tuberculosis from Tanzania. PLoS Negl Trop Dis. 2017;11:e0005817 pubmed publisher
	Galle Treger L, Suzuki Y, Patel N, Sankaranarayanan I, Aron J, Maazi H, et al. Nicotinic acetylcholine receptor agonist attenuates ILC2-dependent airway hyperreactivity. Nat Commun. 2016;7:13202 pubmed publisher
	Wang Y, Jiang X, Zhu J, Dan Yue -, Zhang X, Wang X, et al. IL-21/IL-21R signaling suppresses intestinal inflammation induced by DSS through regulation of Th responses in lamina propria in mice. Sci Rep. 2016;6:31881 pubmed publisher
	Shami A, Knutsson A, DunÃ©r P, Rauch U, Bengtsson E, Tengryd C, et al. Dystrophin deficiency reduces atherosclerotic plaque development in ApoE-null mice. Sci Rep. 2015;5:13904 pubmed publisher

product information

Antigen :

IL-5

Apps. Abbrev. :

Intracellular Staining for Flow Cytometry

Cat # :

504303

Clone :

TRFK5

Item :

PE anti-mouse/human IL-5

Isotype :

Rat IgG1, κ

Other Names :

Interleukin-5, Eosinophil differentiation factor (EDF), Eosinophil colony stimulating factor (Eo-CSF), B cell growth factor-2 (BCGF-2), B cell differentiation factor for IgM (BCDF-m), IgA enhancing factor (IgA-EF), T-cell replacing factor-1 (TRF-1)

Size :

25 μg

Price (USD) :

96 USD

Reactivity :

Mouse, Human

Clonality :

Monoclonal

Host :

Rat

Conjugate/Tag/Label :

Immunogen :

Partially-purified T cell clone supernatant

Application Notes :

ELISA 1,3,4 or ELISPOT 2,6 Capture: The purified TRFK5 antibody is useful as a capture antibody for a sandwich ELISA or ELISPOT assay, when used in conjunction with biotinylated TRFK4 antibody (Cat. No. 504402) as the detection antibody for mouse IL-5, or used in conjunction with biotinylated JES1-5A10 antibody (Cat. No. 501002) as the detection antibody for human IL-5. The Ultra-LEAF purified antibody is suggested for ELISPOT capture. Flow Cytometry: The fluorochrome-labeled TRFK51 antibody is useful for intracellular immunofluorescent staining and flow cytometric analysis to identify IL-5-producing cells within mixed cell populations. Neutralization 1,9 : The Ultra-LEAF purified antibody (Endotoxin 0.1 EU/ug, Azide-Free, 0.2 um filtered) is recommended for neutralization of human IL-5 bioactivity (Cat. Nos. 504313-504318). Additional reported applications (for the relevant formats) include: Western blotting, immunohistochemical staining of formalin-fixed paraffin-embedded tissue sections 8 and paraformaldehyde-fixed, saponin-treated frozen tissue sections 7 , and immunocytochemistry. Note: For testing human IL-5 in serum or plasma, BioLegend s ELISA Max Sets (Cat. No. 430401 and 430404) are specially developed and recommended. For testing mouse IL-5 in serum, plasma or supernatant, BioLegend s ELISA MAX Sets (Cat. Nos. 431201 and 431206) are specially developed and recommended.

more info or order :

BioLegend product webpage