product summary
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company name :
BioLegend
other brands :
Babco, Signet, Sternberger Monoclonals, Senetek, Covance
product type :
antibody
product name :
Ultra-LEAF™ Purified anti-mouse IL-4
catalog :
504136
quantity :
25 mg
price :
1408 USD
clonality :
monoclonal
host :
rat
conjugate :
nonconjugated
clone name :
11B11
reactivity :
mouse
application :
ELISA, immunohistochemistry, immunocytochemistry, neutralization, immunoprecipitation, flow cytometry
more info or order :
citations: 57
Published Application/Species/Sample/DilutionReference
  • flow cytometry; mouse; loading ...; fig 4d
Lin X, Twelkmeyer T, Zhu D, Zhang L, Zhao Y, Zhang C, et al. Homeostatic regulation of T follicular helper and antibody response to particle antigens by IL-1Ra of medullary sinus macrophage origin. Proc Natl Acad Sci U S A. 2021;118: pubmed publisher
  • flow cytometry; mouse; loading ...; fig 1a
Hang S, Paik D, Yao L, Kim E, Jamma T, Lu J, et al. Bile acid metabolites control TH17 and Treg cell differentiation. Nature. 2019;576:143-148 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 6c
Qian L, Bajana S, Georgescu C, Peng V, Wang H, Adrianto I, et al. Suppression of ILC2 differentiation from committed T cell precursors by E protein transcription factors. J Exp Med. 2019;216:884-899 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 1f
Burton O, Tamayo J, Stranks A, Koleoglou K, Oettgen H. Allergen-specific IgG antibody signaling through FcγRIIb promotes food tolerance. J Allergy Clin Immunol. 2018;141:189-201.e3 pubmed publisher
Czopik A, McNamee E, Vaughn V, Huang X, Bang I, Clark T, et al. HIF-2α-dependent induction of miR-29a restrains TH1 activity during T cell dependent colitis. Nat Commun. 2024;15:8042 pubmed publisher
Kurmi K, Liang D, van de Ven R, Georgiev P, Gassaway B, Han S, et al. Metabolic modulation of mitochondrial mass during CD4+ T cell activation. Cell Chem Biol. 2023;30:1064-1075.e8 pubmed publisher
Vereertbrugghen A, Pizzano M, Sabbione F, Keitelman I, Shiromizu C, Aguilar D, et al. An ocular Th1 immune response promotes corneal nerve damage independently of the development of corneal epitheliopathy. J Neuroinflammation. 2023;20:120 pubmed publisher
Aso K, Kono M, Kanda M, Kudo Y, Sakiyama K, Hisada R, et al. Itaconate ameliorates autoimmunity by modulating T cell imbalance via metabolic and epigenetic reprogramming. Nat Commun. 2023;14:984 pubmed publisher
Ogbechi J, Huang Y, Clanchy F, Pantazi E, Topping L, Darlington L, et al. Modulation of immune cell function, IDO expression and kynurenine production by the quorum sensor 2-heptyl-3-hydroxy-4-quinolone (PQS). Front Immunol. 2022;13:1001956 pubmed publisher
Kanno T, Miyako K, Nakajima T, Yokoyama S, Sasamoto S, Asou H, et al. SCD2-mediated cooperative activation of IRF3-IRF9 regulatory circuit controls type I interferon transcriptome in CD4+ T cells. Front Immunol. 2022;13:904875 pubmed publisher
Glaubitz J, Wilden A, Golchert J, Homuth G, V xf6 lker U, Br xf6 ker B, et al. In mouse chronic pancreatitis CD25+FOXP3+ regulatory T cells control pancreatic fibrosis by suppression of the type 2 immune response. Nat Commun. 2022;13:4502 pubmed publisher
Jou E, Rodriguez Rodriguez N, Ferreira A, Jolin H, Clark P, Sawmynaden K, et al. An innate IL-25-ILC2-MDSC axis creates a cancer-permissive microenvironment for Apc mutation-driven intestinal tumorigenesis. Sci Immunol. 2022;7:eabn0175 pubmed publisher
Zhao M, Ren K, Xiong X, Xin Y, Zou Y, Maynard J, et al. Epithelial STAT6 O-GlcNAcylation drives a concerted anti-helminth alarmin response dependent on tuft cell hyperplasia and Gasdermin C. Immunity. 2022;55:623-638.e5 pubmed publisher
Okubo A, Uchida Y, Higashi Y, Sato T, Ogawa Y, Ryuge A, et al. CD147 Is Essential for the Development of Psoriasis via the Induction of Th17 Cell Differentiation. Int J Mol Sci. 2021;23: pubmed publisher
Hao L, Mao Y, Park J, Bae E, Park B. Repurposing the anthelmintic praziquantel to treat psoriasis. Br J Pharmacol. 2021;178:4726-4740 pubmed publisher
Guo P, Yang L, Zhang M, Zhang Y, Tong Y, Cao Y, et al. A Monocyte-Orchestrated IFN-I-to-IL-4 Cytokine Axis Instigates Protumoral Macrophages and Thwarts Poly(I:C) Therapy. J Immunol. 2021;207:408-420 pubmed publisher
Ding X, Chang Y, Wang S, Yan D, Yao J, Zhu G. Transcriptomic Analysis of the Effect of GAT-2 Deficiency on Differentiation of Mice Naïve T Cells Into Th1 Cells In Vitro. Front Immunol. 2021;12:667136 pubmed publisher
Jia X, Zhai T, Qu C, Ye J, Zhao J, Liu X, et al. Metformin Reverses Hashimoto's Thyroiditis by Regulating Key Immune Events. Front Cell Dev Biol. 2021;9:685522 pubmed publisher
Montes De Oca M, de Labastida Rivera F, Winterford C, Frame T, Ng S, Amante F, et al. IL-27 signalling regulates glycolysis in Th1 cells to limit immunopathology during infection. PLoS Pathog. 2020;16:e1008994 pubmed publisher
Xia X, Cao G, Sun G, Zhu L, Tian Y, Song Y, et al. GLS1-mediated glutaminolysis unbridled by MALT1 protease promotes psoriasis pathogenesis. J Clin Invest. 2020;130:5180-5196 pubmed publisher
Imanishi T, Unno M, Kobayashi W, Yoneda N, Akira S, Saito T. mTORC1 Signaling Controls TLR2-Mediated T-Cell Activation by Inducing TIRAP Expression. Cell Rep. 2020;32:107911 pubmed publisher
Price C, Jones S, Mihelcic M, Santic M, Abu Kwaik Y. Paradoxical Pro-inflammatory Responses by Human Macrophages to an Amoebae Host-Adapted Legionella Effector. Cell Host Microbe. 2020;27:571-584.e7 pubmed publisher
Williams G, Marmion D, Schonhoff A, Jurkuvenaite A, Won W, Standaert D, et al. T cell infiltration in both human multiple system atrophy and a novel mouse model of the disease. Acta Neuropathol. 2020;139:855-874 pubmed publisher
Wang L, Shen E, Luo L, Rabe H, Wang Q, Yin J, et al. Control of Germinal Center Localization and Lineage Stability of Follicular Regulatory T Cells by the Blimp1 Transcription Factor. Cell Rep. 2019;29:1848-1861.e6 pubmed publisher
Clancy Thompson E, Chen G, LaMarche N, Ali L, Jeong H, Crowley S, et al. Transnuclear mice reveal Peyer's patch iNKT cells that regulate B-cell class switching to IgG1. EMBO J. 2019;38:e101260 pubmed publisher
Amir M, Chaudhari S, Wang R, Campbell S, Mosure S, Chopp L, et al. REV-ERBα Regulates TH17 Cell Development and Autoimmunity. Cell Rep. 2018;25:3733-3749.e8 pubmed publisher
He Z, Zhang J, Huang Z, Du Q, Li N, Zhang Q, et al. Sumoylation of RORγt regulates TH17 differentiation and thymocyte development. Nat Commun. 2018;9:4870 pubmed publisher
Wu B, Zhang S, Guo Z, Wang G, Zhang G, Xie L, et al. RAS P21 Protein Activator 3 (RASA3) Specifically Promotes Pathogenic T Helper 17 Cell Generation by Repressing T-Helper-2-Cell-Biased Programs. Immunity. 2018;49:886-898.e5 pubmed publisher
Rolot M, Dougall A, Chetty A, Javaux J, Chen T, Xiao X, et al. Helminth-induced IL-4 expands bystander memory CD8+ T cells for early control of viral infection. Nat Commun. 2018;9:4516 pubmed publisher
Singh A, Khare P, Obaid A, Conlon K, Basrur V, Depinho R, et al. SUMOylation of ROR-γt inhibits IL-17 expression and inflammation via HDAC2. Nat Commun. 2018;9:4515 pubmed publisher
Shimada K, Porritt R, Markman J, O Rourke J, Wakita D, Noval Rivas M, et al. T-Cell-Intrinsic Receptor Interacting Protein 2 Regulates Pathogenic T Helper 17 Cell Differentiation. Immunity. 2018;49:873-885.e7 pubmed publisher
Obata Ninomiya K, Ishiwata K, Nakano H, Endo Y, Ichikawa T, Onodera A, et al. CXCR6+ST2+ memory T helper 2 cells induced the expression of major basic protein in eosinophils to reduce the fecundity of helminth. Proc Natl Acad Sci U S A. 2018;115:E9849-E9858 pubmed publisher
Fang D, Cui K, Mao K, Hu G, Li R, Zheng M, et al. Transient T-bet expression functionally specifies a distinct T follicular helper subset. J Exp Med. 2018;215:2705-2714 pubmed publisher
Deason K, Troutman T, Jain A, Challa D, Mandraju R, Brewer T, et al. BCAP links IL-1R to the PI3K-mTOR pathway and regulates pathogenic Th17 cell differentiation. J Exp Med. 2018;215:2413-2428 pubmed publisher
Stathopoulou C, Gangaplara A, Mallett G, Flomerfelt F, Liniany L, Knight D, et al. PD-1 Inhibitory Receptor Downregulates Asparaginyl Endopeptidase and Maintains Foxp3 Transcription Factor Stability in Induced Regulatory T Cells. Immunity. 2018;49:247-263.e7 pubmed publisher
Miller M, Shehat M, Alcedo K, Spinel L, SOULAKOVA J, Tigno Aranjuez J. Frontline Science: RIP2 promotes house dust mite-induced allergic airway inflammation. J Leukoc Biol. 2018;104:447-459 pubmed publisher
Rogers M, Lamens K, Shafagati N, Johnson M, Oury T, Joyce S, et al. CD4+ Regulatory T Cells Exert Differential Functions during Early and Late Stages of the Immune Response to Respiratory Viruses. J Immunol. 2018;201:1253-1266 pubmed publisher
Arnold I, Artola Borán M, Tallón de Lara P, Kyburz A, Taube C, OTTEMANN K, et al. Eosinophils suppress Th1 responses and restrict bacterially induced gastrointestinal inflammation. J Exp Med. 2018;215:2055-2072 pubmed publisher
Sen S, He Z, Ghosh S, DERY K, Yang L, Zhang J, et al. PRMT1 Plays a Critical Role in Th17 Differentiation by Regulating Reciprocal Recruitment of STAT3 and STAT5. J Immunol. 2018;201:440-450 pubmed publisher
Tanaka S, Pfleger C, Lai J, Roan F, Sun S, Ziegler S. KAP1 Regulates Regulatory T Cell Function and Proliferation in Both Foxp3-Dependent and -Independent Manners. Cell Rep. 2018;23:796-807 pubmed publisher
Zhang J, He Z, Sen S, Wang F, Zhang Q, Sun Z. TCF-1 Inhibits IL-17 Gene Expression To Restrain Th17 Immunity in a Stage-Specific Manner. J Immunol. 2018;200:3397-3406 pubmed publisher
Kling J, Jordan M, Pitt L, Meiners J, Thanh Tran T, Tran L, et al. Temporal Regulation of Natural Killer T Cell Interferon Gamma Responses by β-Catenin-Dependent and -Independent Wnt Signaling. Front Immunol. 2018;9:483 pubmed publisher
May S, Owen H, Phesse T, Greenow K, Jones G, Blackwood A, et al. Mbd2 enables tumourigenesis within the intestine while preventing tumour-promoting inflammation. J Pathol. 2018;245:270-282 pubmed publisher
Chyuan I, Tsai H, Wu C, Sung C, Hsu P. TRAIL-Mediated Suppression of T Cell Receptor Signaling Inhibits T Cell Activation and Inflammation in Experimental Autoimmune Encephalomyelitis. Front Immunol. 2018;9:15 pubmed publisher
Xiao X, Fan Y, Li J, Zhang X, Lou X, Dou Y, et al. Guidance of super-enhancers in regulation of IL-9 induction and airway inflammation. J Exp Med. 2018;215:559-574 pubmed publisher
Maseda D, Johnson E, Nyhoff L, Baron B, Kojima F, Wilhelm A, et al. mPGES1-Dependent Prostaglandin E2 (PGE2) Controls Antigen-Specific Th17 and Th1 Responses by Regulating T Autocrine and Paracrine PGE2 Production. J Immunol. 2018;200:725-736 pubmed publisher
Shi B, Geng J, Wang Y, Wei H, Walters B, Li W, et al. Foxp1 Negatively Regulates T Follicular Helper Cell Differentiation and Germinal Center Responses by Controlling Cell Migration and CTLA-4. J Immunol. 2018;200:586-594 pubmed publisher
Ibrahim M, Scozzi D, Toth K, Ponti D, Kreisel D, Menna C, et al. Naive CD4+ T Cells Carrying a TLR2 Agonist Overcome TGF-β-Mediated Tumor Immune Evasion. J Immunol. 2018;200:847-856 pubmed publisher
Read K, Powell M, Baker C, Sreekumar B, Ringel Scaia V, Bachus H, et al. Integrated STAT3 and Ikaros Zinc Finger Transcription Factor Activities Regulate Bcl-6 Expression in CD4+ Th Cells. J Immunol. 2017;199:2377-2387 pubmed publisher
Clancy Thompson E, Chen G, Tyler P, Servos M, Barisa M, Brennan P, et al. Monoclonal Invariant NKT (iNKT) Cell Mice Reveal a Role for Both Tissue of Origin and the TCR in Development of iNKT Functional Subsets. J Immunol. 2017;199:159-171 pubmed publisher
Suzuki T, Murakami S, Biswal S, Sakaguchi S, Harigae H, Yamamoto M, et al. Systemic Activation of NRF2 Alleviates Lethal Autoimmune Inflammation in Scurfy Mice. Mol Cell Biol. 2017;37: pubmed publisher
Fu G, Xu Q, Qiu Y, Jin X, Xu T, Dong S, et al. Suppression of Th17 cell differentiation by misshapen/NIK-related kinase MINK1. J Exp Med. 2017;214:1453-1469 pubmed publisher
Tian L, Goldstein A, Wang H, Ching Lo H, Sun Kim I, Welte T, et al. Mutual regulation of tumour vessel normalization and immunostimulatory reprogramming. Nature. 2017;544:250-254 pubmed publisher
Jiang J, Liu C, Liu M, Shen Y, Hu X, Wang Q, et al. OX40 signaling is involved in the autoactivation of CD4+CD28- T cells and contributes to the pathogenesis of autoimmune arthritis. Arthritis Res Ther. 2017;19:67 pubmed publisher
Ulrich B, Verdan F, McKenzie A, Kaplan M, Olson M. STAT3 Activation Impairs the Stability of Th9 Cells. J Immunol. 2017;198:2302-2309 pubmed publisher
Levine A, Hemmers S, Baptista A, Schizas M, Faire M, Moltedo B, et al. Suppression of lethal autoimmunity by regulatory T cells with a single TCR specificity. J Exp Med. 2017;214:609-622 pubmed publisher
Reynolds L, Harcus Y, Smith K, Webb L, Hewitson J, Ross E, et al. MyD88 signaling inhibits protective immunity to the gastrointestinal helminth parasite Heligmosomoides polygyrus. J Immunol. 2014;193:2984-93 pubmed publisher
product information
Antigen :
IL-4
Apps. Abbrev. :
ELISA Capture, CyTOF®, ELISPOT Capture, Neut, ICC, IP, IHC
Cat # :
504136
Clone :
11B11
Item :
Ultra-LEAF™ Purified anti-mouse IL-4
Isotype :
Rat IgG1, κ
Other Names :
Interleukin-4, Ia inducing factor (IaIF), B cell stimulating factor-1 (BSF-1), Hodgkin's cell growth factor (HCGF), Mast cell growth factor-2 (MCGF-2), Macrophage fusion factor (MFF), T cell growth factor-2 (TCGF-2)
Size :
25 mg
Price (USD) :
1408 USD
Reactivity :
Mouse
Clonality :
Monoclonal
Host :
Rat
Conjugate/Tag/Label :
ULEAF
Immunogen :
Partially purified native mouse IL-4
Application Notes :
ELISA^1,2,10,13 or ELISPOT^5 Capture: The purified 11B11 antibody is useful as the capture antibody in a sandwich ELISA or ELISPOT assay, when used in conjunction with the biotinylated BVD6-24G2 antibody (Cat. No. 504202) as the detecting antibody and recombinant mouse IL-4 (Cat. No. 575609) as the standard. The LEAF™ purified antibody is suggested for ELISPOT capture.Neutralization^1-2,9,12: The 11B11 antibody can neutralize the bioactivity of natural or recombinant IL-4. The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for neutralization of mouse IL-4 bioactivity in vivo and in vitro (Cat. No. 504108).Additional reported applications (for the relevant formats) include: immunoprecipitation^16, immunohistochemical staining of formalin-fixed paraffin-embedded tissue sections^8 and paraformaldehyde-fixed, saponin-treated frozen tissue sections^6,7, and immunocytochemistry^4.Note: For testing mouse IL-4 in serum, plasma or supernatant, BioLegend's ELISA Max™ Sets (Cat. No. 431101 to 431106) are specially developed and recommended.;
more info or order :
company information
BioLegend
8999 BioLegend Way
San Diego, CA 92121
customerserv@biolegend.com
https://www.biolegend.com
headquarters: USA