PerCP/Cyanine5.5 anti-mouse IL-4 | BioLegend 504123 product information

product summary

company name :

BioLegend

other brands :

Babco, Signet, Sternberger Monoclonals, Senetek, Covance

product type :

antibody

product name :

PerCP/Cyanine5.5 anti-mouse IL-4

catalog :

504123

quantity :

25 μg

price :

129 USD

clonality :

monoclonal

host :

rat

conjugate :

PerCP/Cy5.5

clone name :

11B11

reactivity :

mouse

application :

flow cytometry

more info or order :

BioLegend product webpage

citations: 11

Published Application/Species/Sample/Dilution	Reference
flow cytometry; mouse; loading ...; fig 5c	Takahashi T, Asano Y, Sugawara K, Yamashita T, Nakamura K, Saigusa R, et al. Epithelial Fli1 deficiency drives systemic autoimmunity and fibrosis: Possible roles in scleroderma. J Exp Med. 2017;214:1129-1151 pubmed publisher
	Zeng Q, Song J, Wang D, Sun X, Xiao Y, Zhang H, et al. Identification of Sorafenib as a Treatment for Type 1 Diabetes. Front Immunol. 2022;13:740805 pubmed publisher
	Faust H, Zhang H, Han J, Wolf M, Jeon O, Sadtler K, et al. IL-17 and immunologically induced senescence regulate response to injury in osteoarthritis. J Clin Invest. 2020;130:5493-5507 pubmed publisher
	Cai W, Kesavan D, Cheng J, Vasudevan A, Wang H, Wan J, et al. Vesicle-Mediated Dendritic Cell Activation in Acinetobacter baumannii Clinical Isolate, which Contributes to Th2 Response. J Immunol Res. 2019;2019:2835256 pubmed publisher
	Steinmann S, Schoedsack M, Heinrich F, Breda P, Ochel A, Tiegs G, et al. Hepatic ILC2 activity is regulated by liver inflammation-induced cytokines and effector CD4+ T cells. Sci Rep. 2020;10:1071 pubmed publisher
	Nakornpakdee Y, Sermswan R, Maneewatchararangsri S, Wongratanacheewin S. Hamster IFN-γ+CD4+ and IL-4+CD4+ T cell responses against leptospires are significantly higher than those of mice. Asian Pac J Allergy Immunol. 2018;36:265-272 pubmed publisher
	Makker P, Duffy S, Lees J, Perera C, Tonkin R, Butovsky O, et al. Characterisation of Immune and Neuroinflammatory Changes Associated with Chemotherapy-Induced Peripheral Neuropathy. PLoS ONE. 2017;12:e0170814 pubmed publisher
	Zhao Y, Zhou M, Gao Y, Liu H, Yang W, Yue J, et al. Shifted T Helper Cell Polarization in a Murine Staphylococcus aureus Mastitis Model. PLoS ONE. 2015;10:e0134797 pubmed publisher
	Charles N, Hardwick D, Daugas E, Illei G, Rivera J. Basophils and the T helper 2 environment can promote the development of lupus nephritis. Nat Med. 2010;16:701-7 pubmed publisher
	Ohnmacht C, Voehringer D. Basophil effector function and homeostasis during helminth infection. Blood. 2009;113:2816-25 pubmed publisher
	Xu G, Liu D, Fan Y, Yang X, Korner H, Fu Y, et al. Lymphotoxin alpha beta 2 (membrane lymphotoxin) is critically important for resistance to Leishmania major infection in mice. J Immunol. 2007;179:5358-66 pubmed

product information

Antigen :

IL-4

Apps. Abbrev. :

Intracellular Staining for Flow Cytometry

Cat # :

504123

Clone :

11B11

Item :

PerCP/Cyanine5.5 anti-mouse IL-4

Isotype :

Rat IgG1, κ

Other Names :

Interleukin-4, Ia inducing factor (IaIF), B cell stimulating factor-1 (BSF-1), Hodgkin's cell growth factor (HCGF), Mast cell growth factor-2 (MCGF-2), Macrophage fusion factor (MFF), T cell growth factor-2 (TCGF-2)

Size :

25 μg

Price (USD) :

129 USD

Reactivity :

Mouse

Clonality :

Monoclonal

Host :

Rat

Conjugate/Tag/Label :

PERCP_CYANINE5.5

Immunogen :

Partially purified native mouse IL-4

Application Notes :

ELISA 1,2,10,13 or ELISPOT 5 Capture: The purified 11B11 antibody is useful as the capture antibody in a sandwich ELISA or ELISPOT assay, when used in conjunction with the biotinylated BVD6-24G2 antibody (Cat. No. 504202) as the detecting antibody and recombinant mouse IL-4 (Cat. No. 575609) as the standard. The LEAF™ purified antibody is suggested for ELISPOT capture. Neutralization 1-2,9,12 : The 11B11 antibody can neutralize the bioactivity of natural or recombinant IL-4. The LEAF™ purified antibody (Endotoxin 0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for neutralization of mouse IL-4 bioactivity in vivo and in vitro (Cat. No. 504108). Additional reported applications (for the relevant formats) include: immunoprecipitation 16 , immunohistochemical staining of formalin-fixed paraffin-embedded tissue sections 8 and paraformaldehyde-fixed, saponin-treated frozen tissue sections 6,7 , and immunocytochemistry 4 . Note: For testing mouse IL-4 in serum, plasma or supernatant, BioLegend s ELISA Max™ Sets (Cat. No. 431101 to 431106) are specially developed and recommended.

more info or order :

BioLegend product webpage