Brilliant Violet 421™ anti-mouse IL-4 | BioLegend 504119 product information

product summary

company name :

BioLegend

other brands :

Babco, Signet, Sternberger Monoclonals, Senetek, Covance

product type :

antibody

product name :

Brilliant Violet 421™ anti-mouse IL-4

catalog :

504119

quantity :

125 μL

price :

193 USD

clonality :

monoclonal

host :

rat

conjugate :

BV421

clone name :

11B11

reactivity :

mouse

application :

flow cytometry

more info or order :

BioLegend product webpage

citations: 6

Reference
Mikami Y, Philips R, Sciumè G, Petermann F, Meylan F, Nagashima H, et al. MicroRNA-221 and -222 modulate intestinal inflammatory Th17 cell response as negative feedback regulators downstream of interleukin-23. Immunity. 2021;54:514-525.e6 pubmed publisher
Yang J, Wang W, Chen Z, Lu S, Yang F, Bi Z, et al. A vaccine targeting the RBD of the S protein of SARS-CoV-2 induces protective immunity. Nature. 2020;586:572-577 pubmed publisher
Vono M, Eberhardt C, Auderset F, Mastelic Gavillet B, Lemeille S, Christensen D, et al. Maternal Antibodies Inhibit Neonatal and Infant Responses to Vaccination by Shaping the Early-Life B Cell Repertoire within Germinal Centers. Cell Rep. 2019;28:1773-1784.e5 pubmed publisher
Komuczki J, Tuzlak S, Friebel E, Hartwig T, Spath S, Rosenstiel P, et al. Fate-Mapping of GM-CSF Expression Identifies a Discrete Subset of Inflammation-Driving T Helper Cells Regulated by Cytokines IL-23 and IL-1β. Immunity. 2019;: pubmed publisher
Schmidleithner L, Thabet Y, Schönfeld E, Köhne M, Sommer D, Abdullah Z, et al. Enzymatic Activity of HPGD in Treg Cells Suppresses Tconv Cells to Maintain Adipose Tissue Homeostasis and Prevent Metabolic Dysfunction. Immunity. 2019;50:1232-1248.e14 pubmed publisher
Shi B, Geng J, Wang Y, Wei H, Walters B, Li W, et al. Foxp1 Negatively Regulates T Follicular Helper Cell Differentiation and Germinal Center Responses by Controlling Cell Migration and CTLA-4. J Immunol. 2018;200:586-594 pubmed publisher

product information

Antigen :

IL-4

Apps. Abbrev. :

Intracellular Staining for Flow Cytometry

Cat # :

504119

Clone :

11B11

Item :

Brilliant Violet 421™ anti-mouse IL-4

Isotype :

Rat IgG1, κ

Other Names :

Interleukin-4, Ia inducing factor (IaIF), B cell stimulating factor-1 (BSF-1), Hodgkin's cell growth factor (HCGF), Mast cell growth factor-2 (MCGF-2), Macrophage fusion factor (MFF), T cell growth factor-2 (TCGF-2)

Size :

125 μL

Price (USD) :

193 USD

Reactivity :

Mouse

Clonality :

Monoclonal

Host :

Rat

Conjugate/Tag/Label :

BV421

Immunogen :

Partially purified native mouse IL-4

Application Notes :

ELISA 1,2,10,13 or ELISPOT 5 Capture: The purified 11B11 antibody is useful as the capture antibody in a sandwich ELISA or ELISPOT assay, when used in conjunction with the biotinylated BVD6-24G2 antibody (Cat. No. 504202) as the detecting antibody and recombinant mouse IL-4 (Cat. No. 575609) as the standard. The LEAF™ purified antibody is suggested for ELISPOT capture. Neutralization 1-2,9,12 : The 11B11 antibody can neutralize the bioactivity of natural or recombinant IL-4. The LEAF™ purified antibody (Endotoxin 0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for neutralization of mouse IL-4 bioactivity in vivo and in vitro (Cat. No. 504108). Additional reported applications (for the relevant formats) include: immunoprecipitation 16 , immunohistochemical staining of formalin-fixed paraffin-embedded tissue sections 8 and paraformaldehyde-fixed, saponin-treated frozen tissue sections 6,7 , and immunocytochemistry 4 . Note: For testing mouse IL-4 in serum, plasma or supernatant, BioLegend s ELISA Max™ Sets (Cat. No. 431101 to 431106) are specially developed and recommended.

more info or order :

BioLegend product webpage