PE/Cyanine7 anti-mouse IL-4 | BioLegend 504117 product information

product summary

company name :

BioLegend

other brands :

Babco, Signet, Sternberger Monoclonals, Senetek, Covance

product type :

antibody

product name :

PE/Cyanine7 anti-mouse IL-4

catalog :

504117

quantity :

25 μg

price :

129 USD

clonality :

monoclonal

host :

rat

conjugate :

PE/Cy7

clone name :

11B11

reactivity :

mouse

application :

flow cytometry

more info or order :

BioLegend product webpage

citations: 18

Published Application/Species/Sample/Dilution	Reference
flow cytometry; mouse; 1:100; loading ...; fig 3d	Yong L, Yu Y, Li B, Ge H, Zhen Q, Mao Y, et al. Calcium/calmodulin-dependent protein kinase IV promotes imiquimod-induced psoriatic inflammation via macrophages and keratinocytes in mice. Nat Commun. 2022;13:4255 pubmed publisher
flow cytometry; mouse; loading ...; fig 6c	Qian L, Bajana S, Georgescu C, Peng V, Wang H, Adrianto I, et al. Suppression of ILC2 differentiation from committed T cell precursors by E protein transcription factors. J Exp Med. 2019;216:884-899 pubmed publisher
flow cytometry; mouse; loading ...; fig 1c	Smith R, Reyes N, Khandelwal P, Schlereth S, Lee H, Masli S, et al. Secondary allergic T cell responses are regulated by dendritic cell-derived thrombospondin-1 in the setting of allergic eye disease. J Leukoc Biol. 2016;100:371-80 pubmed publisher
	Wang W, Yao J, Chen Z, Sun Y, Shi Y, Wei Y, et al. Methnaridine is an orally bioavailable, fast-killing and long-acting antimalarial agent that cures Plasmodium infections in mice. Br J Pharmacol. 2020;177:5569-5579 pubmed publisher
	Starkl P, Watzenboeck M, Popov L, Zahalka S, Hladik A, Lakovits K, et al. IgE Effector Mechanisms, in Concert with Mast Cells, Contribute to Acquired Host Defense against Staphylococcusaureus. Immunity. 2020;53:793-804.e9 pubmed publisher
	Jin J, Jung I, Moon S, Jeon S, Jeong S, Sonn S, et al. CD137 Signaling Regulates Acute Colitis via RALDH2-Expressing CD11b-CD103+ DCs. Cell Rep. 2020;30:4124-4136.e5 pubmed publisher
	Kim T, Park G, Kim J, Lim S, Kim J, Im K, et al. CD160 serves as a negative regulator of NKT cells in acute hepatic injury. Nat Commun. 2019;10:3258 pubmed publisher
	Kisielow J, Obermair F, Kopf M. Deciphering CD4+ T cell specificity using novel MHC-TCR chimeric receptors. Nat Immunol. 2019;20:652-662 pubmed publisher
	Morimoto Y, Hirahara K, Kiuchi M, Wada T, Ichikawa T, Kanno T, et al. Amphiregulin-Producing Pathogenic Memory T Helper 2 Cells Instruct Eosinophils to Secrete Osteopontin and Facilitate Airway Fibrosis. Immunity. 2018;49:134-150.e6 pubmed publisher
	Jiang J, Liu C, Liu M, Shen Y, Hu X, Wang Q, et al. OX40 signaling is involved in the autoactivation of CD4+CD28- T cells and contributes to the pathogenesis of autoimmune arthritis. Arthritis Res Ther. 2017;19:67 pubmed publisher
	Cho S, Roh K, Park J, Park Y, Lee M, Cho S, et al. Hydrolysis of Hyaluronic Acid in Lymphedematous Tissue Alleviates Fibrogenesis via TH1 Cell-Mediated Cytokine Expression. Sci Rep. 2017;7:35 pubmed publisher
	Alves C, Ober BlÃ¶baum J, Brouwers Haspels I, Asmawidjaja P, Mus A, Razawy W, et al. Dendritic Cell-Specific Deletion of Î²-Catenin Results in Fewer Regulatory T-Cells without Exacerbating Autoimmune Collagen-Induced Arthritis. PLoS ONE. 2015;10:e0142972 pubmed publisher
	Voigt A, Esfandiary L, Nguyen C. Sexual dimorphism in an animal model of SjÃ¶gren's syndrome: a potential role for Th17 cells. Biol Open. 2015;4:1410-9 pubmed publisher
	Sharma S, Chintala N, Vadrevu S, Patel J, Karbowniczek M, Markiewski M. Pulmonary alveolar macrophages contribute to the premetastatic niche by suppressing antitumor T cell responses in the lungs. J Immunol. 2015;194:5529-38 pubmed publisher
	Shibata K, Yamada H, Nakamura M, Hatano S, Katsuragi Y, Kominami R, et al. IFN-?-producing and IL-17-producing ?? T cells differentiate at distinct developmental stages in murine fetal thymus. J Immunol. 2014;192:2210-8 pubmed publisher
	Charles N, Hardwick D, Daugas E, Illei G, Rivera J. Basophils and the T helper 2 environment can promote the development of lupus nephritis. Nat Med. 2010;16:701-7 pubmed publisher
	Ohnmacht C, Voehringer D. Basophil effector function and homeostasis during helminth infection. Blood. 2009;113:2816-25 pubmed publisher
	Xu G, Liu D, Fan Y, Yang X, Korner H, Fu Y, et al. Lymphotoxin alpha beta 2 (membrane lymphotoxin) is critically important for resistance to Leishmania major infection in mice. J Immunol. 2007;179:5358-66 pubmed

product information

Antigen :

IL-4

Apps. Abbrev. :

Intracellular Staining for Flow Cytometry

Cat # :

504117

Clone :

11B11

Item :

PE/Cyanine7 anti-mouse IL-4

Isotype :

Rat IgG1, κ

Other Names :

Interleukin-4, Ia inducing factor (IaIF), B cell stimulating factor-1 (BSF-1), Hodgkin's cell growth factor (HCGF), Mast cell growth factor-2 (MCGF-2), Macrophage fusion factor (MFF), T cell growth factor-2 (TCGF-2)

Size :

25 μg

Price (USD) :

129 USD

Reactivity :

Mouse

Clonality :

Monoclonal

Host :

Rat

Conjugate/Tag/Label :

PE_CYANINE7

Immunogen :

Partially purified native mouse IL-4

Application Notes :

ELISA 1,2,10,13 or ELISPOT 5 Capture: The purified 11B11 antibody is useful as the capture antibody in a sandwich ELISA or ELISPOT assay, when used in conjunction with the biotinylated BVD6-24G2 antibody (Cat. No. 504202) as the detecting antibody and recombinant mouse IL-4 (Cat. No. 575609) as the standard. The LEAF™ purified antibody is suggested for ELISPOT capture. Neutralization 1-2,9,12 : The 11B11 antibody can neutralize the bioactivity of natural or recombinant IL-4. The LEAF™ purified antibody (Endotoxin 0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for neutralization of mouse IL-4 bioactivity in vivo and in vitro (Cat. No. 504108). Additional reported applications (for the relevant formats) include: immunoprecipitation 16 , immunohistochemical staining of formalin-fixed paraffin-embedded tissue sections 8 and paraformaldehyde-fixed, saponin-treated frozen tissue sections 6,7 , and immunocytochemistry 4 . Note: For testing mouse IL-4 in serum, plasma or supernatant, BioLegend s ELISA Max™ Sets (Cat. No. 431101 to 431106) are specially developed and recommended.

more info or order :

BioLegend product webpage