LEAF™ Purified anti-mouse IL-4 | BioLegend 504108 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

BioLegend

other brands :

Babco, Signet, Sternberger Monoclonals, Senetek, Covance

product type :

antibody

product name :

LEAF™ Purified anti-mouse IL-4

catalog :

504108

quantity :

500 μg

price :

250 USD

clonality :

monoclonal

host :

rat

conjugate :

nonconjugated

clone name :

11B11

reactivity :

mouse

application :

immunohistochemistry, immunocytochemistry, neutralization, immunoprecipitation, flow cytometry, blocking or activating experiments

citations: 3

Published Application/Species/Sample/Dilution	Reference
flow cytometry; mouse; loading ...; fig 3e	Fu G, Xu Q, Qiu Y, Jin X, Xu T, Dong S, et al. Suppression of Th17 cell differentiation by misshapen/NIK-related kinase MINK1. J Exp Med. 2017;214:1453-1469 pubmed publisher
flow cytometry; mouse; fig s2a	Beyaz S, Kim J, Pinello L, Xifaras M, Hu Y, Huang J, et al. The histone demethylase UTX regulates the lineage-specific epigenetic program of invariant natural killer T cells. Nat Immunol. 2017;18:184-195 pubmed publisher
blocking or activating experiments; mouse; loading ...; fig 7a	Janssen E, Tohme M, Hedayat M, Leick M, Kumari S, Ramesh N, et al. A DOCK8-WIP-WASp complex links T cell receptors to the actin cytoskeleton. J Clin Invest. 2016;126:3837-3851 pubmed publisher

product information

Antigen :

IL-4

Apps. Abbrev. :

ELISA Capture, CyTOF®, ELISPOT Capture, Neut, ICC, IP, IHC

Cat # :

504108

Clone :

11B11

Item :

LEAF™ Purified anti-mouse IL-4

Isotype :

Rat IgG1, κ

Other Names :

Interleukin-4, IaIF, BSF-1, HCGF, MCGF-2, MFF, TCGF-2

Size :

500 μg

Price (USD) :

250 USD

Reactivity :

Mouse

Clonality :

Monoclonal

Host :

Rat

Conjugate/Tag/Label :

LEAF

Immunogen :

Partially purified native mouse IL-4

Application Notes :

ELISA 1,2,10,13 or ELISPOT 5 Capture: The purified 11B11 antibody is useful as the capture antibody in a sandwich ELISA or ELISPOT assay, when used in conjunction with the biotinylated BVD6-24G2 antibody (Cat. No. 504202) as the detecting antibody and recombinant mouse IL-4 (Cat. No. 575609) as the standard. The LEAF purified antibody is suggested for ELISPOT capture. Neutralization 1-2,9,12 : The 11B11 antibody can neutralize the bioactivity of natural or recombinant IL-4. The LEAF purified antibody (Endotoxin 0.1 EU/ug, Azide-Free, 0.2 um filtered) is recommended for neutralization of mouse IL-4 bioactivity in vivo and in vitro (Cat. No. 504108). Additional reported applications (for the relevant formats) include: immunoprecipitation 16 , immunohistochemical staining of formalin-fixed paraffin-embedded tissue sections 8 and paraformaldehyde-fixed, saponin-treated frozen tissue sections 6,7 , and immunocytochemistry 4 . Note: For testing mouse IL-4 in serum, plasma or supernatant, BioLegend's ELISA Max Sets (Cat. No. 431101 to 431106) are specially developed and recommended.