PerCP/Cyanine5.5 anti-mouse IL-2 | BioLegend 503821 product information

product summary

company name :

BioLegend

other brands :

Babco, Signet, Sternberger Monoclonals, Senetek, Covance

product type :

antibody

product name :

PerCP/Cyanine5.5 anti-mouse IL-2

catalog :

503821

quantity :

25 μg

price :

118 USD

clonality :

monoclonal

host :

rat

conjugate :

nonconjugated

clone name :

JES6-5H4

reactivity :

mouse

application :

flow cytometry

more info or order :

BioLegend product webpage

citations: 9

Reference
Hodgins B, Pillet S, Landry N, Ward B. Prime-pull vaccination with a plant-derived virus-like particle influenza vaccine elicits a broad immune response and protects aged mice from death and frailty after challenge. Immun Ageing. 2019;16:27 pubmed publisher
Zhou J, Peng H, Li K, Qu K, Wang B, Wu Y, et al. Liver-Resident NK Cells Control Antiviral Activity of Hepatic T Cells via the PD-1-PD-L1 Axis. Immunity. 2019;50:403-417.e4 pubmed publisher
Chiou N, Kageyama R, Ansel K. Selective Export into Extracellular Vesicles and Function of tRNA Fragments during T Cell Activation. Cell Rep. 2018;25:3356-3370.e4 pubmed publisher
Tsai S, Clemente Casares X, Zhou A, Lei H, Ahn J, Chan Y, et al. Insulin Receptor-Mediated Stimulation Boosts T Cell Immunity during Inflammation and Infection. Cell Metab. 2018;28:922-934.e4 pubmed publisher
Yamamoto M, Seki Y, Iwai K, Ko I, Martin A, Tsuji N, et al. Ontogeny and localization of the cells produce IL-2 in healthy animals. Cytokine. 2013;61:831-41 pubmed publisher
Aldhamen Y, Seregin S, Schuldt N, Rastall D, Liu C, Godbehere S, et al. Vaccines expressing the innate immune modulator EAT-2 elicit potent effector memory T lymphocyte responses despite pre-existing vaccine immunity. J Immunol. 2012;189:1349-59 pubmed publisher
Fourcade J, Sun Z, Pagliano O, Guillaume P, Luescher I, Sander C, et al. CD8(+) T cells specific for tumor antigens can be rendered dysfunctional by the tumor microenvironment through upregulation of the inhibitory receptors BTLA and PD-1. Cancer Res. 2012;72:887-96 pubmed publisher
Aldhamen Y, Appledorn D, Seregin S, Liu C, Schuldt N, Godbehere S, et al. Expression of the SLAM family of receptors adapter EAT-2 as a novel strategy for enhancing beneficial immune responses to vaccine antigens. J Immunol. 2011;186:722-32 pubmed publisher
Fourcade J, Sun Z, Benallaoua M, Guillaume P, Luescher I, Sander C, et al. Upregulation of Tim-3 and PD-1 expression is associated with tumor antigen-specific CD8+ T cell dysfunction in melanoma patients. J Exp Med. 2010;207:2175-86 pubmed publisher

product information

Antigen :

IL-2

Apps. Abbrev. :

Intracellular Staining for Flow Cytometry

Cat # :

503821

Clone :

JES6-5H4

Item :

PerCP/Cyanine5.5 anti-mouse IL-2

Isotype :

Rat IgG2b, κ

Other Names :

Interleukin-2, T cell growth factor (TCGF), Eosinophil differentiation factor (EDF), Killer cell helper factor (KHF), Macrophage-activating factor for cytotoxicity I (MAF-C I), Thymocyte differentiation factor (TDF)

Size :

25 μg

Price (USD) :

118 USD

Reactivity :

mouse

Clonality :

Monoclonal

Host :

Rat

Conjugate/Tag/Label :

PERCP_CYANINE5.5

Immunogen :

E. coli -expressed, recombinant mouse IL-2

Application Notes :

ELISA Detection 1-3 or ELISPOT Detection 4-6 : The biotinylated JES6-5H4 antibody is useful as a detection antibody for a sandwich ELISA or ELISPOT assay, when used in conjunction with the purified JES6-1A12 antibody (Cat. Nos. 503701 503702) as capture antibody and recombinant mouse IL-2 (Cat. No. 575409) as the standard. Flow Cytometry 8-10 : The fluorochrome-labeled JES6-5H4 antibody is useful for intracellular immunofluorescent staining and flow cytometric analysis to identify IL-2 -producing cells within mixed cell populations. Neutralization 1,7 : The Ultra-LEAF™ purified antibody (Endotoxin in vivo and in vitro (Cat. No. 503845-503850)) is recommended for neutralization. Additional reported applications (for the relevant formats) include : immunoprecipitation 1 , immunohistochemical staining of paraformaldehyde-fixed, saponin-treated frozen tissue sections 2 , in vivo capture 7 , and immunocytochemistry. Note: For testing mouse IL-2 in serum, plasma or supernatant, BioLegend s ELISA MAX™ Sets (Cat. No. 431001 431004) are specially developed and recommended.

more info or order :

BioLegend product webpage