LEAF™ Purified anti-mouse IL-2 | BioLegend 503812 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

BioLegend

other brands :

Babco, Signet, Sternberger Monoclonals, Senetek, Covance

product type :

antibody

product name :

LEAF™ Purified anti-mouse IL-2

catalog :

503812

quantity :

500 μg

price :

260 USD

clonality :

monoclonal

host :

rat

conjugate :

nonconjugated

clone name :

JES6-5H4

reactivity :

mouse

application :

immunohistochemistry, neutralization, immunoprecipitation, flow cytometry

citations: 23

Published Application/Species/Sample/Dilution	Reference
flow cytometry; mouse; loading ...; fig 3b	Klotz L, Kuzmanov I, Hucke S, Gross C, Posevitz V, Dreykluft A, et al. B7-H1 shapes T-cell-mediated brain endothelial cell dysfunction and regional encephalitogenicity in spontaneous CNS autoimmunity. Proc Natl Acad Sci U S A. 2016;113:E6182-E6191 pubmed
flow cytometry; mouse; fig 6	Kritikou J, Dahlberg C, Baptista M, Wagner A, Banerjee P, Gwalani L, et al. IL-2 in the tumor microenvironment is necessary for Wiskott-Aldrich syndrome protein deficient NK cells to respond to tumors in vivo. Sci Rep. 2016;6:30636 pubmed publisher
flow cytometry; mouse; fig 2	Apostolidis S, RodrÃguez RodrÃguez N, SuÃ¡rez Fueyo A, Dioufa N, Ozcan E, CrispÃn J, et al. Phosphatase PP2A is requisite for the function of regulatory T cells. Nat Immunol. 2016;17:556-64 pubmed publisher
flow cytometry; mouse; loading ...; fig 5f	Metz P, Lopez J, Kim S, Akimoto K, Ohno S, Chang J. Regulation of Asymmetric Division by Atypical Protein Kinase C Influences Early Specification of CD8(+) T Lymphocyte Fates. Sci Rep. 2016;6:19182 pubmed publisher
flow cytometry; mouse; fig 3b	Moguche A, Shafiani S, Clemons C, Larson R, Dinh C, Higdon L, et al. ICOS and Bcl6-dependent pathways maintain a CD4 T cell population with memory-like properties during tuberculosis. J Exp Med. 2015;212:715-28 pubmed publisher
flow cytometry; mouse	Misumi I, Whitmire J. B cell depletion curtails CD4+ T cell memory and reduces protection against disseminating virus infection. J Immunol. 2014;192:1597-608 pubmed publisher
	Yamamoto T, Lee P, Vodnala S, Gurusamy D, Kishton R, Yu Z, et al. T cells genetically engineered to overcome death signaling enhance adoptive cancer immunotherapy. J Clin Invest. 2019;129:1551-1565 pubmed publisher
	Li J, He Y, Hao J, Ni L, Dong C. High Levels of Eomes Promote Exhaustion of Anti-tumor CD8+ T Cells. Front Immunol. 2018;9:2981 pubmed publisher
	Tilstra J, Avery L, Menk A, Gordon R, Smita S, Kane L, et al. Kidney-infiltrating T cells in murine lupus nephritis are metabolically and functionally exhausted. J Clin Invest. 2018;128:4884-4897 pubmed publisher
	Oldstone M, Ware B, Horton L, Welch M, Aiolfi R, Zarpellon A, et al. Lymphocytic choriomeningitis virus Clone 13 infection causes either persistence or acute death dependent on IFN-1, cytotoxic T lymphocytes (CTLs), and host genetics. Proc Natl Acad Sci U S A. 2018;115:E7814-E7823 pubmed publisher
	Kennedy S, Johnson A, Bharrhan S, Lindestam Arlehamn C, Xu J, Garforth S, et al. Identification of Mycobacterial Ribosomal Proteins as Targets for CD4+ T Cells That Enhance Protective Immunity in Tuberculosis. Infect Immun. 2018;86: pubmed publisher
	Andargachew R, Martinez R, Kolawole E, Evavold B. CD4 T Cell Affinity Diversity Is Equally Maintained during Acute and Chronic Infection. J Immunol. 2018;201:19-30 pubmed publisher
	Valentine K, Davini D, Lawrence T, Mullins G, Manansala M, Al Kuhlani M, et al. CD8 Follicular T Cells Promote B Cell Antibody Class Switch in Autoimmune Disease. J Immunol. 2018;201:31-40 pubmed publisher
	Milam A, Bartleson J, Donermeyer D, Horvath S, Durai V, Raju S, et al. Tuning T Cell Signaling Sensitivity Alters the Behavior of CD4+ T Cells during an Immune Response. J Immunol. 2018;200:3429-3437 pubmed publisher
	Chancellor A, Tocheva A, Cave Ayland C, Tezera L, White A, Al Dulayymi J, et al. CD1b-restricted GEM T cell responses are modulated by Mycobacterium tuberculosis mycolic acid meromycolate chains. Proc Natl Acad Sci U S A. 2017;114:E10956-E10964 pubmed publisher
	Nirschl C, Suárez Fariñas M, Izar B, Prakadan S, Dannenfelser R, Tirosh I, et al. IFNγ-Dependent Tissue-Immune Homeostasis Is Co-opted in the Tumor Microenvironment. Cell. 2017;170:127-141.e15 pubmed publisher
	Wu S, Niknafs Y, Kim S, Oravecz Wilson K, Zajac C, Toubai T, et al. A Critical Analysis of the Role of SNARE Protein SEC22B in Antigen Cross-Presentation. Cell Rep. 2017;19:2645-2656 pubmed publisher
	Ghoneim H, Fan Y, Moustaki A, Abdelsamed H, Dash P, Dogra P, et al. De Novo Epigenetic Programs Inhibit PD-1 Blockade-Mediated T Cell Rejuvenation. Cell. 2017;170:142-157.e19 pubmed publisher
	Deng L, Kim J, Chang T, Zhang H, Mohan T, Champion J, et al. Protein nanoparticle vaccine based on flagellin carrier fused to influenza conserved epitopes confers full protection against influenza A virus challenge. Virology. 2017;509:82-89 pubmed publisher
	Seki S, Stevenson M, Rosen A, Arandjelovic S, Gemta L, Bullock T, et al. Lineage-Specific Metabolic Properties and Vulnerabilities of T Cells in the Demyelinating Central Nervous System. J Immunol. 2017;198:4607-4617 pubmed publisher
	Levine A, Hemmers S, Baptista A, Schizas M, Faire M, Moltedo B, et al. Suppression of lethal autoimmunity by regulatory T cells with a single TCR specificity. J Exp Med. 2017;214:609-622 pubmed publisher
	Liu W, Kang S, Huang Z, Wu C, Jin H, Maine C, et al. A miR-155-Peli1-c-Rel pathway controls the generation and function of T follicular helper cells. J Exp Med. 2016;213:1901-19 pubmed publisher
	Walker J, Raué H, Slifka M. Characterization of CD8+ T cell function and immunodominance generated with an H2O2-inactivated whole-virus vaccine. J Virol. 2012;86:13735-44 pubmed publisher

product information

Antigen :

IL-2

Apps. Abbrev. :

ELISA, Neut, Intracellular Staining for Flow Cytometry, IP, IHC

Cat # :

503812

Clone :

JES6-5H4

Item :

LEAF™ Purified anti-mouse IL-2

Isotype :

Rat IgG2b, κ

Other Names :

Interleukin-2, T cell growth factor (TCGF), Eosinophil differentiation factor (EDF), Killer cell helper factor (KHF), Macrophage-activating factor for cytotoxicity I (MAF-C I), Thymocyte differentiation factor (TDF)

Size :

500 μg

Price (USD) :

260 USD

Reactivity :

Mouse

Clonality :

Monoclonal

Host :

Rat

Conjugate/Tag/Label :

LEAF

Immunogen :

E. coli -expressed, recombinant mouse IL-2

Application Notes :

ELISA Detection 1-3 or ELISPOT Detection 4-6 : The biotinylated JES6-5H4 antibody is useful as a detection antibody for a sandwich ELISA or ELISPOT assay, when used in conjunction with the purified JES6-1A12 antibody (Cat. Nos. 503701 503702) as capture antibody and recombinant mouse IL-2 (Cat. No. 575409) as the standard. Flow Cytometry 8-10 : The fluorochrome-labeled JES6-5H4 antibody is useful for intracellular immunofluorescent staining and flow cytometric analysis to identify IL-2 -producing cells within mixed cell populations. Neutralization 1,7 : The Ultra-LEAF™ purified antibody (Endotoxin in vivo and in vitro (Cat. No. 503845-503850)) is recommended for neutralization. Additional reported applications (for the relevant formats) include : immunoprecipitation 1 , immunohistochemical staining of paraformaldehyde-fixed, saponin-treated frozen tissue sections 2 , in vivo capture 7 , and immunocytochemistry. Note: For testing mouse IL-2 in serum, plasma or supernatant, BioLegend's ELISA MAX™ Sets (Cat. No. 431001 431004) are specially developed and recommended.