APC anti-mouse IL-2 | BioLegend 503809 product information

product summary

company name :

BioLegend

other brands :

Babco, Signet, Sternberger Monoclonals, Senetek, Covance

product type :

antibody

product name :

APC anti-mouse IL-2

catalog :

503809

quantity :

25 μg

price :

99 USD

clonality :

monoclonal

host :

rat

conjugate :

APC

clone name :

JES6-5H4

reactivity :

mouse

application :

flow cytometry

more info or order :

BioLegend product webpage

citations: 21

Published Application/Species/Sample/Dilution	Reference
flow cytometry; mouse	OlguÃn J, FernÃ¡ndez J, Salinas N, JuÃ¡rez I, Rodriguez Sosa M, Campuzano J, et al. Adoptive transfer of CD4(+)Foxp3(+) regulatory T cells to C57BL/6J mice during acute infection with Toxoplasma gondii down modulates the exacerbated Th1 immune response. Microbes Infect. 2015;17:586-95 pubmed publisher
	Souza S, Splitt S, Sànchez Arcila J, Alvarez J, Wilson J, Wizzard S, et al. Genetic mapping reveals Nfkbid as a central regulator of humoral immunity to Toxoplasma gondii. PLoS Pathog. 2021;17:e1010081 pubmed publisher
	Bosnjak B, Odak I, Barros Martins J, Sandrock I, Hammerschmidt S, Permanyer M, et al. Intranasal Delivery of MVA Vector Vaccine Induces Effective Pulmonary Immunity Against SARS-CoV-2 in Rodents. Front Immunol. 2021;12:772240 pubmed publisher
	Marangoni F, Zhakyp A, Corsini M, Geels S, Carrizosa E, Thelen M, et al. Expansion of tumor-associated Treg cells upon disruption of a CTLA-4-dependent feedback loop. Cell. 2021;184:3998-4015.e19 pubmed publisher
	Mitchell J, Lund M, Starmer J, Ge K, Magnuson T, Shpargel K, et al. UTX promotes CD8+ T cell-mediated antiviral defenses but reduces T cell durability. Cell Rep. 2021;35:108966 pubmed publisher
	Hanna C, Ashhurst A, Quan D, Maxwell J, Britton W, Payne R. Synthetic protein conjugate vaccines provide protection against Mycobacterium tuberculosis in mice. Proc Natl Acad Sci U S A. 2021;118: pubmed publisher
	Wang N, Yi H, Fang L, Jin J, Ma Q, Shen Y, et al. CD226 Attenuates Treg Proliferation via Akt and Erk Signaling in an EAE Model. Front Immunol. 2020;11:1883 pubmed publisher
	Xiao S, Bod L, Pochet N, KOTA S, Hu D, Madi A, et al. Checkpoint Receptor TIGIT Expressed on Tim-1+ B Cells Regulates Tissue Inflammation. Cell Rep. 2020;32:107892 pubmed publisher
	Thomas A, Dong Y, Beskid N, Garcia A, Adams A, Babensee J. Brief exposure to hyperglycemia activates dendritic cells in vitro and in vivo. J Cell Physiol. 2020;235:5120-5129 pubmed publisher
	Andersen L, Gülich A, Alteneder M, Preglej T, Orola M, Dhele N, et al. The Transcription Factor MAZR/PATZ1 Regulates the Development of FOXP3+ Regulatory T Cells. Cell Rep. 2019;29:4447-4459.e6 pubmed publisher
	Piper C, Rosser E, Oleinika K, Nistala K, Krausgruber T, Rendeiro A, et al. Aryl Hydrocarbon Receptor Contributes to the Transcriptional Program of IL-10-Producing Regulatory B Cells. Cell Rep. 2019;29:1878-1892.e7 pubmed publisher
	Misumi I, Starmer J, Uchimura T, Beck M, Magnuson T, Whitmire J. Obesity Expands a Distinct Population of T Cells in Adipose Tissue and Increases Vulnerability to Infection. Cell Rep. 2019;27:514-524.e5 pubmed publisher
	Uchimura T, Oyama Y, Deng M, Guo H, Wilson J, Rampanelli E, et al. The Innate Immune Sensor NLRC3 Acts as a Rheostat that Fine-Tunes T Cell Responses in Infection and Autoimmunity. Immunity. 2018;49:1049-1061.e6 pubmed publisher
	Waight J, Chand D, Dietrich S, Gombos R, Horn T, Gonzalez A, et al. Selective FcγR Co-engagement on APCs Modulates the Activity of Therapeutic Antibodies Targeting T Cell Antigens. Cancer Cell. 2018;33:1033-1047.e5 pubmed publisher
	Shimba A, Cui G, Tani ichi S, Ogawa M, Abe S, Okazaki F, et al. Glucocorticoids Drive Diurnal Oscillations in T Cell Distribution and Responses by Inducing Interleukin-7 Receptor and CXCR4. Immunity. 2018;48:286-298.e6 pubmed publisher
	Tritama E, Riani C, Rudiansyah I, Hidayat A, Kharisnaeni S, Retnoningrum D. Evaluation of alum-based adjuvant on the immunogenicity of salmonella enterica serovar typhi conjugates vaccines. Hum Vaccin Immunother. 2018;14:1524-1529 pubmed publisher
	Maschmeyer P, Petkau G, Siracusa F, Zimmermann J, Zügel F, Kühl A, et al. Selective targeting of pro-inflammatory Th1 cells by microRNA-148a-specific antagomirs in vivo. J Autoimmun. 2018;89:41-52 pubmed publisher
	Jackson C, Kochel C, Nirschl C, Durham N, Ruzevick J, Alme A, et al. Systemic Tolerance Mediated by Melanoma Brain Tumors Is Reversible by Radiotherapy and Vaccination. Clin Cancer Res. 2016;22:1161-72 pubmed publisher
	Burrack K, Montgomery S, Homann D, Morrison T. CD8+ T cells control Ross River virus infection in musculoskeletal tissues of infected mice. J Immunol. 2015;194:678-89 pubmed publisher
	Ma Z, LeBard D, Loverde S, Sharp K, Klein M, Discher D, et al. TCR triggering by pMHC ligands tethered on surfaces via poly(ethylene glycol) depends on polymer length. PLoS ONE. 2014;9:e112292 pubmed publisher
	Lei F, Haque R, Xiong X, Song J. Directed differentiation of induced pluripotent stem cells towards T lymphocytes. J Vis Exp. 2012;:e3986 pubmed publisher

product information

Antigen :

IL-2

Apps. Abbrev. :

Intracellular Staining for Flow Cytometry

Cat # :

503809

Clone :

JES6-5H4

Item :

APC anti-mouse IL-2

Isotype :

Rat IgG2b, κ

Other Names :

Interleukin-2, T cell growth factor (TCGF), Eosinophil differentiation factor (EDF), Killer cell helper factor (KHF), Macrophage-activating factor for cytotoxicity I (MAF-C I), Thymocyte differentiation factor (TDF)

Size :

25 μg

Price (USD) :

99 USD

Reactivity :

Mouse

Clonality :

Monoclonal

Host :

Rat

Conjugate/Tag/Label :

APC

Immunogen :

E. coli -expressed, recombinant mouse IL-2

Application Notes :

ELISA Detection 1-3 or ELISPOT Detection 4-6 : The biotinylated JES6-5H4 antibody is useful as a detection antibody for a sandwich ELISA or ELISPOT assay, when used in conjunction with the purified JES6-1A12 antibody (Cat. Nos. 503701 503702) as capture antibody and recombinant mouse IL-2 (Cat. No. 575409) as the standard. Flow Cytometry 8-10 : The fluorochrome-labeled JES6-5H4 antibody is useful for intracellular immunofluorescent staining and flow cytometric analysis to identify IL-2 -producing cells within mixed cell populations. Neutralization 1,7 : The Ultra-LEAF™ purified antibody (Endotoxin in vivo and in vitro (Cat. No. 503845-503850)) is recommended for neutralization. Additional reported applications (for the relevant formats) include : immunoprecipitation 1 , immunohistochemical staining of paraformaldehyde-fixed, saponin-treated frozen tissue sections 2 , in vivo capture 7 , and immunocytochemistry. Note: For testing mouse IL-2 in serum, plasma or supernatant, BioLegend s ELISA MAX™ Sets (Cat. No. 431001 431004) are specially developed and recommended.

more info or order :

BioLegend product webpage