product summary
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company name :
BioLegend
other brands :
Babco, Signet, Sternberger Monoclonals, Senetek, Covance
product type :
antibody
product name :
PE anti-mouse IL-2
catalog :
503808
quantity :
100 μg
price :
220 USD
clonality :
monoclonal
host :
rat
conjugate :
PE
clone name :
JES6-5H4
reactivity :
mouse
application :
flow cytometry
more info or order :
citations: 35
Published Application/Species/Sample/DilutionReference
  • flow cytometry; mouse; loading ...
Bonilla W, Kirchhammer N, Marx A, Kallert S, Krzyzaniak M, Lu M, et al. Heterologous arenavirus vector prime-boost overrules self-tolerance for efficient tumor-specific CD8 T cell attack. Cell Rep Med. 2021;2:100209 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 3o
Sockolosky J, Trotta E, Parisi G, Picton L, Su L, Le A, et al. Selective targeting of engineered T cells using orthogonal IL-2 cytokine-receptor complexes. Science. 2018;359:1037-1042 pubmed publisher
  • flow cytometry; mouse; loading ...
Hu Y, Kim J, He K, Wan Q, Kim J, Flach M, et al. Scramblase TMEM16F terminates T cell receptor signaling to restrict T cell exhaustion. J Exp Med. 2016;213:2759-2772 pubmed
  • flow cytometry; mouse
White C, Villarino N, Sloan S, Ganusov V, Schmidt N. Plasmodium suppresses expansion of T cell responses to heterologous infections. J Immunol. 2015;194:697-708 pubmed publisher
Duan Q, Ding J, Li F, Liu X, Zhao Y, Yu H, et al. Sirtuin 5 is Dispensable for CD8+ T Cell Effector and Memory Differentiation. Front Cell Dev Biol. 2021;9:761193 pubmed publisher
Tanaka Y, Nakao A, Miyake Y, Higashi Y, Tanigaki R, Kataoka T. Small Molecule Inhibitors Targeting Nuclear Factor κB Activation Markedly Reduce Expression of Interleukin-2, but Not Interferon-γ, Induced by Phorbol Esters and Calcium Ionophores. Int J Mol Sci. 2021;22: pubmed publisher
Klarquist J, Cross E, Thompson S, Willett B, Aldridge D, Caffrey Carr A, et al. B cells promote CD8 T cell primary and memory responses to subunit vaccines. Cell Rep. 2021;36:109591 pubmed publisher
Marangoni F, Zhakyp A, Corsini M, Geels S, Carrizosa E, Thelen M, et al. Expansion of tumor-associated Treg cells upon disruption of a CTLA-4-dependent feedback loop. Cell. 2021;184:3998-4015.e19 pubmed publisher
Li J, Hardy K, Olshansky M, Barugahare A, Gearing L, Prier J, et al. KDM6B-dependent chromatin remodeling underpins effective virus-specific CD8+ T cell differentiation. Cell Rep. 2021;34:108839 pubmed publisher
Zhuang Z, Lai X, Sun J, Chen Z, Zhang Z, Dai J, et al. Mapping and role of T cell response in SARS-CoV-2-infected mice. J Exp Med. 2021;218: pubmed publisher
Hirai T, Yang Y, Zenke Y, Li H, Chaudhri V, De La Cruz Diaz J, et al. Competition for Active TGFβ Cytokine Allows for Selective Retention of Antigen-Specific Tissue- Resident Memory T Cells in the Epidermal Niche. Immunity. 2021;54:84-98.e5 pubmed publisher
Ringel A, Drijvers J, Baker G, Catozzi A, García Cañaveras J, Gassaway B, et al. Obesity Shapes Metabolism in the Tumor Microenvironment to Suppress Anti-Tumor Immunity. Cell. 2020;183:1848-1866.e26 pubmed publisher
Corrado M, Edwards Hicks J, Villa M, Flachsmann L, Sanin D, Jacobs M, et al. Dynamic Cardiolipin Synthesis Is Required for CD8+ T Cell Immunity. Cell Metab. 2020;32:981-995.e7 pubmed publisher
Sun Y, Preiss N, Valenteros K, Kamal Y, Usherwood Y, Frost H, et al. Zbtb20 Restrains CD8 T Cell Immunometabolism and Restricts Memory Differentiation and Antitumor Immunity. J Immunol. 2020;205:2649-2666 pubmed publisher
Acharya N, Madi A, Zhang H, Klapholz M, Escobar G, Dulberg S, et al. Endogenous Glucocorticoid Signaling Regulates CD8+ T Cell Differentiation and Development of Dysfunction in the Tumor Microenvironment. Immunity. 2020;53:658-671.e6 pubmed publisher
Laczkó D, Hogan M, Toulmin S, Hicks P, Lederer K, Gaudette B, et al. A Single Immunization with Nucleoside-Modified mRNA Vaccines Elicits Strong Cellular and Humoral Immune Responses against SARS-CoV-2 in Mice. Immunity. 2020;53:724-732.e7 pubmed publisher
Wen J, Wang Y, Valentine K, Dos Santos Alves R, Xu Z, Regla Nava J, et al. CD4+ T Cells Cross-Reactive with Dengue and Zika Viruses Protect against Zika Virus Infection. Cell Rep. 2020;31:107566 pubmed publisher
Varikuti S, Singh B, Volpedo G, Ahirwar D, Jha B, Saljoughian N, et al. Ibrutinib treatment inhibits breast cancer progression and metastasis by inducing conversion of myeloid-derived suppressor cells to dendritic cells. Br J Cancer. 2020;122:1005-1013 pubmed publisher
Steinmann S, Schoedsack M, Heinrich F, Breda P, Ochel A, Tiegs G, et al. Hepatic ILC2 activity is regulated by liver inflammation-induced cytokines and effector CD4+ T cells. Sci Rep. 2020;10:1071 pubmed publisher
Lercher A, Bhattacharya A, Popa A, Caldera M, Schlapansky M, Baazim H, et al. Type I Interferon Signaling Disrupts the Hepatic Urea Cycle and Alters Systemic Metabolism to Suppress T Cell Function. Immunity. 2019;51:1074-1087.e9 pubmed publisher
Kim C, Jadhav R, Gustafson C, Smithey M, Hirsch A, Uhrlaub J, et al. Defects in Antiviral T Cell Responses Inflicted by Aging-Associated miR-181a Deficiency. Cell Rep. 2019;29:2202-2216.e5 pubmed publisher
Elong Ngono A, Shresta S. Cross-Reactive T Cell Immunity to Dengue and Zika Viruses: New Insights Into Vaccine Development. Front Immunol. 2019;10:1316 pubmed publisher
Hirai T, Zenke Y, Yang Y, Bartholin L, Beura L, Masopust D, et al. Keratinocyte-Mediated Activation of the Cytokine TGF-β Maintains Skin Recirculating Memory CD8+ T Cells. Immunity. 2019;50:1249-1261.e5 pubmed publisher
Sivick K, Desbien A, Glickman L, Reiner G, Corrales L, Surh N, et al. Magnitude of Therapeutic STING Activation Determines CD8+ T Cell-Mediated Anti-tumor Immunity. Cell Rep. 2018;25:3074-3085.e5 pubmed publisher
Sade Feldman M, Yizhak K, Bjorgaard S, Ray J, de Boer C, Jenkins R, et al. Defining T Cell States Associated with Response to Checkpoint Immunotherapy in Melanoma. Cell. 2018;175:998-1013.e20 pubmed publisher
Lu Y, Wang Q, Xue G, Bi E, Ma X, Wang A, et al. Th9 Cells Represent a Unique Subset of CD4+ T Cells Endowed with the Ability to Eradicate Advanced Tumors. Cancer Cell. 2018;33:1048-1060.e7 pubmed publisher
Page N, Klimek B, De Roo M, Steinbach K, Soldati H, Lemeille S, et al. Expression of the DNA-Binding Factor TOX Promotes the Encephalitogenic Potential of Microbe-Induced Autoreactive CD8+ T Cells. Immunity. 2018;48:937-950.e8 pubmed publisher
Maschmeyer P, Petkau G, Siracusa F, Zimmermann J, Zügel F, Kühl A, et al. Selective targeting of pro-inflammatory Th1 cells by microRNA-148a-specific antagomirs in vivo. J Autoimmun. 2018;89:41-52 pubmed publisher
Ding Z, Habtetsion T, Cao Y, Li T, Liu C, Kuczma M, et al. Adjuvant IL-7 potentiates adoptive T cell therapy by amplifying and sustaining polyfunctional antitumor CD4+ T cells. Sci Rep. 2017;7:12168 pubmed publisher
Yan Q, Yang C, Fu Q, Chen Z, Liu S, Fu D, et al. Scaffold protein JLP mediates TCR-initiated CD4+T cell activation and CD154 expression. Mol Immunol. 2017;87:258-266 pubmed publisher
Kim M, van Dolleweerd C, Copland A, Paul M, Hofmann S, Webster G, et al. Molecular engineering and plant expression of an immunoglobulin heavy chain scaffold for delivery of a dengue vaccine candidate. Plant Biotechnol J. 2017;15:1590-1601 pubmed publisher
Knocke S, Fleischmann Mundt B, Saborowski M, Manns M, Kuhnel F, Wirth T, et al. Tailored Tumor Immunogenicity Reveals Regulation of CD4 and CD8 T Cell Responses against Cancer. Cell Rep. 2016;17:2234-2246 pubmed publisher
Singer M, Wang C, Cong L, Marjanovic N, Kowalczyk M, Zhang H, et al. A Distinct Gene Module for Dysfunction Uncoupled from Activation in Tumor-Infiltrating T Cells. Cell. 2016;166:1500-1511.e9 pubmed publisher
Charlton J, Tsoukatou D, Mamalaki C, Chatzidakis I. Programmed death 1 regulates memory phenotype CD4 T cell accumulation, inhibits expansion of the effector memory phenotype subset and modulates production of effector cytokines. PLoS ONE. 2015;10:e0119200 pubmed publisher
Haring J, Jing X, Bollenbacher Reilley J, Xue H, Leonard W, Harty J. Constitutive expression of IL-7 receptor alpha does not support increased expansion or prevent contraction of antigen-specific CD4 or CD8 T cells following Listeria monocytogenes infection. J Immunol. 2008;180:2855-62 pubmed
product information
Antigen :
IL-2
Apps. Abbrev. :
Intracellular Staining for Flow Cytometry
Cat # :
503808
Clone :
JES6-5H4
Item :
PE anti-mouse IL-2
Isotype :
Rat IgG2b, κ
Other Names :
Interleukin-2, T cell growth factor (TCGF), Eosinophil differentiation factor (EDF), Killer cell helper factor (KHF), Macrophage-activating factor for cytotoxicity I (MAF-C I), Thymocyte differentiation factor (TDF)
Size :
100 μg
Price (USD) :
220 USD
Reactivity :
Mouse
Clonality :
Monoclonal
Host :
Rat
Conjugate/Tag/Label :
PE
Immunogen :
E. coli -expressed, recombinant mouse IL-2
Application Notes :
ELISA Detection 1-3 or ELISPOT Detection 4-6 : The biotinylated JES6-5H4 antibody is useful as a detection antibody for a sandwich ELISA or ELISPOT assay, when used in conjunction with the purified JES6-1A12 antibody (Cat. Nos. 503701 503702) as capture antibody and recombinant mouse IL-2 (Cat. No. 575409) as the standard. Flow Cytometry 8-10 : The fluorochrome-labeled JES6-5H4 antibody is useful for intracellular immunofluorescent staining and flow cytometric analysis to identify IL-2 -producing cells within mixed cell populations. Neutralization 1,7 : The Ultra-LEAF™ purified antibody (Endotoxin in vivo and in vitro (Cat. No. 503845-503850)) is recommended for neutralization. Additional reported applications (for the relevant formats) include : immunoprecipitation 1 , immunohistochemical staining of paraformaldehyde-fixed, saponin-treated frozen tissue sections 2 , in vivo capture 7 , and immunocytochemistry. Note: For testing mouse IL-2 in serum, plasma or supernatant, BioLegend s ELISA MAX™ Sets (Cat. No. 431001 431004) are specially developed and recommended.
more info or order :
company information
BioLegend
8999 BioLegend Way
San Diego, CA 92121
customerserv@biolegend.com
https://www.biolegend.com
headquarters: USA