Biotin anti-mouse IL-2 | BioLegend 503804 product information

product summary

company name :

BioLegend

other brands :

Babco, Signet, Sternberger Monoclonals, Senetek, Covance

product type :

antibody

product name :

Biotin anti-mouse IL-2

catalog :

503804

quantity :

500 μg

price :

253 USD

clonality :

monoclonal

host :

rat

conjugate :

biotin

clone name :

JES6-5H4

reactivity :

mouse

application :

ELISA

more info or order :

BioLegend product webpage

citations: 14

Published Application/Species/Sample/Dilution	Reference
ELISA; mouse; fig 2f	Ozdilek A, Paschall A, Dookwah M, Tiemeyer M, Avci F. Host protein glycosylation in nucleic acid vaccines as a potential hurdle in vaccine design for nonviral pathogens. Proc Natl Acad Sci U S A. 2020;117:1280-1282 pubmed publisher
ELISA; mouse; fig 3j	Bar On Y, Charpak Amikam Y, Glasner A, Isaacson B, Duev Cohen A, Tsukerman P, et al. NKp46 Recognizes the Sigma1 Protein of Reovirus: Implications for Reovirus-Based Cancer Therapy. J Virol. 2017;91: pubmed publisher
	Anthony S, Van Braeckel Budimir N, Moioffer S, Van de Wall S, Shan Q, Vijay R, et al. Protective function and durability of mouse lymph node-resident memory CD8+ T cells. elife. 2021;10: pubmed publisher
	Wang N, Yi H, Fang L, Jin J, Ma Q, Shen Y, et al. CD226 Attenuates Treg Proliferation via Akt and Erk Signaling in an EAE Model. Front Immunol. 2020;11:1883 pubmed publisher
	Xiao S, Bod L, Pochet N, KOTA S, Hu D, Madi A, et al. Checkpoint Receptor TIGIT Expressed on Tim-1+ B Cells Regulates Tissue Inflammation. Cell Rep. 2020;32:107892 pubmed publisher
	Thomas A, Dong Y, Beskid N, Garcia A, Adams A, Babensee J. Brief exposure to hyperglycemia activates dendritic cells in vitro and in vivo. J Cell Physiol. 2020;235:5120-5129 pubmed publisher
	Andersen L, Gülich A, Alteneder M, Preglej T, Orola M, Dhele N, et al. The Transcription Factor MAZR/PATZ1 Regulates the Development of FOXP3+ Regulatory T Cells. Cell Rep. 2019;29:4447-4459.e6 pubmed publisher
	Piper C, Rosser E, Oleinika K, Nistala K, Krausgruber T, Rendeiro A, et al. Aryl Hydrocarbon Receptor Contributes to the Transcriptional Program of IL-10-Producing Regulatory B Cells. Cell Rep. 2019;29:1878-1892.e7 pubmed publisher
	Komuczki J, Tuzlak S, Friebel E, Hartwig T, Spath S, Rosenstiel P, et al. Fate-Mapping of GM-CSF Expression Identifies a Discrete Subset of Inflammation-Driving T Helper Cells Regulated by Cytokines IL-23 and IL-1β. Immunity. 2019;: pubmed publisher
	Peng Y. Forced expression of IL-7R promotes CD8 T cell cytotoxicity to self antigen. PLoS ONE. 2017;12:e0188112 pubmed publisher
	Rodriguez S, Jiang M, Bujo H, Allen P. Self-pMHCII complexes are variably expressed in the thymus and periphery independent of mRNA expression but dependent on the activation state of the APCs. Mol Immunol. 2015;63:428-36 pubmed publisher
	Guy C, Vignali K, Temirov J, Bettini M, Overacre A, Smeltzer M, et al. Distinct TCR signaling pathways drive proliferation and cytokine production in T cells. Nat Immunol. 2013;14:262-70 pubmed publisher
	Wang X, Szymczak Workman A, Gravano D, Workman C, Green D, Vignali D. Preferential control of induced regulatory T cell homeostasis via a Bim/Bcl-2 axis. Cell Death Dis. 2012;3:e270 pubmed publisher
	Kao H, Lin J, Littman D, Shaw A, Allen P. Regulated movement of CD4 in and out of the immunological synapse. J Immunol. 2008;181:8248-57 pubmed

product information

Antigen :

IL-2

Apps. Abbrev. :

ELISA Detection, ELISPOT Detection, Intracellular Staining for Flow Cytometry

Cat # :

503804

Clone :

JES6-5H4

Item :

Biotin anti-mouse IL-2

Isotype :

Rat IgG2b, κ

Other Names :

Interleukin-2, T cell growth factor (TCGF), Eosinophil differentiation factor (EDF), Killer cell helper factor (KHF), Macrophage-activating factor for cytotoxicity I (MAF-C I), Thymocyte differentiation factor (TDF)

Size :

500 μg

Price (USD) :

253 USD

Reactivity :

Mouse

Clonality :

Monoclonal

Host :

Rat

Conjugate/Tag/Label :

BIOTIN

Immunogen :

E. coli -expressed, recombinant mouse IL-2

Application Notes :

ELISA Detection 1-3 or ELISPOT Detection 4-6 : The biotinylated JES6-5H4 antibody is useful as a detection antibody for a sandwich ELISA or ELISPOT assay, when used in conjunction with the purified JES6-1A12 antibody (Cat. Nos. 503701 503702) as capture antibody and recombinant mouse IL-2 (Cat. No. 575409) as the standard. Flow Cytometry 8-10 : The fluorochrome-labeled JES6-5H4 antibody is useful for intracellular immunofluorescent staining and flow cytometric analysis to identify IL-2 -producing cells within mixed cell populations. Neutralization 1,7 : The Ultra-LEAF™ purified antibody (Endotoxin in vivo and in vitro (Cat. No. 503845-503850)) is recommended for neutralization. Additional reported applications (for the relevant formats) include : immunoprecipitation 1 , immunohistochemical staining of paraformaldehyde-fixed, saponin-treated frozen tissue sections 2 , in vivo capture 7 , and immunocytochemistry. Note: For testing mouse IL-2 in serum, plasma or supernatant, BioLegend s ELISA MAX™ Sets (Cat. No. 431001 431004) are specially developed and recommended.

more info or order :

BioLegend product webpage