Biotin anti-mouse IL-2 | BioLegend 503803 product information

product summary

company name :

BioLegend

other brands :

Babco, Signet, Sternberger Monoclonals, Senetek, Covance

product type :

antibody

product name :

Biotin anti-mouse IL-2

catalog :

503803

quantity :

50 μg

price :

101 USD

clonality :

monoclonal

host :

rat

conjugate :

biotin

clone name :

JES6-5H4

reactivity :

mouse

application :

ELISA

more info or order :

BioLegend product webpage

citations: 8

Reference
Anthony S, Van Braeckel Budimir N, Moioffer S, Van de Wall S, Shan Q, Vijay R, et al. Protective function and durability of mouse lymph node-resident memory CD8+ T cells. elife. 2021;10: pubmed publisher
Ozdilek A, Paschall A, Dookwah M, Tiemeyer M, Avci F. Host protein glycosylation in nucleic acid vaccines as a potential hurdle in vaccine design for nonviral pathogens. Proc Natl Acad Sci U S A. 2020;117:1280-1282 pubmed publisher
Komuczki J, Tuzlak S, Friebel E, Hartwig T, Spath S, Rosenstiel P, et al. Fate-Mapping of GM-CSF Expression Identifies a Discrete Subset of Inflammation-Driving T Helper Cells Regulated by Cytokines IL-23 and IL-1β. Immunity. 2019;: pubmed publisher
Peng Y. Forced expression of IL-7R promotes CD8 T cell cytotoxicity to self antigen. PLoS ONE. 2017;12:e0188112 pubmed publisher
Rodriguez S, Jiang M, Bujo H, Allen P. Self-pMHCII complexes are variably expressed in the thymus and periphery independent of mRNA expression but dependent on the activation state of the APCs. Mol Immunol. 2015;63:428-36 pubmed publisher
Guy C, Vignali K, Temirov J, Bettini M, Overacre A, Smeltzer M, et al. Distinct TCR signaling pathways drive proliferation and cytokine production in T cells. Nat Immunol. 2013;14:262-70 pubmed publisher
Wang X, Szymczak Workman A, Gravano D, Workman C, Green D, Vignali D. Preferential control of induced regulatory T cell homeostasis via a Bim/Bcl-2 axis. Cell Death Dis. 2012;3:e270 pubmed publisher
Kao H, Lin J, Littman D, Shaw A, Allen P. Regulated movement of CD4 in and out of the immunological synapse. J Immunol. 2008;181:8248-57 pubmed

product information

Antigen :

IL-2

Apps. Abbrev. :

ELISA Detection, ELISPOT Detection, Intracellular Staining for Flow Cytometry

Cat # :

503803

Clone :

JES6-5H4

Item :

Biotin anti-mouse IL-2

Isotype :

Rat IgG2b, κ

Other Names :

Interleukin-2, T cell growth factor (TCGF), Eosinophil differentiation factor (EDF), Killer cell helper factor (KHF), Macrophage-activating factor for cytotoxicity I (MAF-C I), Thymocyte differentiation factor (TDF)

Size :

50 μg

Price (USD) :

101 USD

Reactivity :

Mouse

Clonality :

Monoclonal

Host :

Rat

Conjugate/Tag/Label :

BIOTIN

Immunogen :

E. coli -expressed, recombinant mouse IL-2

Application Notes :

ELISA Detection 1-3 or ELISPOT Detection 4-6 : The biotinylated JES6-5H4 antibody is useful as a detection antibody for a sandwich ELISA or ELISPOT assay, when used in conjunction with the purified JES6-1A12 antibody (Cat. Nos. 503701 503702) as capture antibody and recombinant mouse IL-2 (Cat. No. 575409) as the standard. Flow Cytometry 8-10 : The fluorochrome-labeled JES6-5H4 antibody is useful for intracellular immunofluorescent staining and flow cytometric analysis to identify IL-2 -producing cells within mixed cell populations. Neutralization 1,7 : The Ultra-LEAF™ purified antibody (Endotoxin in vivo and in vitro (Cat. No. 503845-503850)) is recommended for neutralization. Additional reported applications (for the relevant formats) include : immunoprecipitation 1 , immunohistochemical staining of paraformaldehyde-fixed, saponin-treated frozen tissue sections 2 , in vivo capture 7 , and immunocytochemistry. Note: For testing mouse IL-2 in serum, plasma or supernatant, BioLegend s ELISA MAX™ Sets (Cat. No. 431001 431004) are specially developed and recommended.

more info or order :

BioLegend product webpage