Babco, Signet, Sternberger Monoclonals, Senetek, Covance

antibody

TotalSeq™-B0945 anti-human TNF-α

502957

10 μg

369 USD

monoclonal

mouse

nonconjugated

MAb11

ICPG

502957

MAb11

TotalSeq™-B0945 anti-human TNF-α

Mouse IgG1, κ

Tumor necrosis factor-α, Cachectin, Necrosin, Macrophage cytotoxic factor (MCF), Differentiation inducing factor (DIF), TNFSF2

10 μg

369 USD

Human

Monoclonal

Mouse

TOTALSEQ_B

E. coli -expressed, recombinant human TNF-α

ELISA or ELISPOT Detection: The biotinylated MAb11 antibody is useful as the detection antibody in a sandwich ELISA or ELISPOT, when used in conjunction with the purified MAb1 antibody (Cat. No. 502802/502804) as the capture antibody.Flow Cytometry^3,5,6,10: The fluorochrome-labeled MAb11 antibody is useful for intracellular and membrane-bound immunofluorescent staining and flow cytometric analysis to identify TNF-a-producing cells within mixed cell populations.Additional reported applications (for the relevant formats) include: neutralization^1,2, immunohistochemical staining of paraformaldehyde-fixed, saponin-treated frozen tissue sections^4 and acetone-fixed frozen tissue sections^8, immunocytochemistry^7, and immunofluorescence^9. The MAb11 antibody can neutralize the bioactivity of natural or recombinant TNF-a.Note: For testing human TNF-a in serum or plasma, BioLegend's ELISA Max™ Sets (Cat. No. 430201 to 430206) are specially developed and recommended. The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for neutralization of human TNF-a bioactivity (Cat. No. 502922).The Purified MAb1 antibody is useful in neutralization^2 and as the capture antibody in a sandwich ELISA or ELISPOT assay, when used in conjunction with the biotinylated MAb11 antibody (Cat. No. 502904/502914) as the detecting antibody.Clone MAb11 cross-reacts to Cat^11;