APC/Cyanine7 anti-human TNF-α | BioLegend 502944 product information

product summary

company name :

BioLegend

other brands :

Babco, Signet, Sternberger Monoclonals, Senetek, Covance

product type :

antibody

product name :

APC/Cyanine7 anti-human TNF-α

catalog :

502944

quantity :

100 tests

price :

329 USD

clonality :

monoclonal

host :

mouse

conjugate :

APC/Cy7

clone name :

MAb11

reactivity :

human

application :

immunocytochemistry, flow cytometry

more info or order :

BioLegend product webpage

citations: 7

Published Application/Species/Sample/Dilution	Reference
flow cytometry; human; loading ...; fig 7a	Kirkling M, Cytlak U, Lau C, Lewis K, Resteu A, Khodadadi Jamayran A, et al. Notch Signaling Facilitates In Vitro Generation of Cross-Presenting Classical Dendritic Cells. Cell Rep. 2018;23:3658-3672.e6 pubmed publisher
	Zhou R, To K, Wong Y, Liu L, Zhou B, Li X, et al. Acute SARS-CoV-2 Infection Impairs Dendritic Cell and T Cell Responses. Immunity. 2020;53:864-877.e5 pubmed publisher
	Juno J, Tan H, Lee W, Reynaldi A, Kelly H, Wragg K, et al. Humoral and circulating follicular helper T cell responses in recovered patients with COVID-19. Nat Med. 2020;26:1428-1434 pubmed publisher
	Angiari S, Runtsch M, Sutton C, Palsson McDermott E, Kelly B, Rana N, et al. Pharmacological Activation of Pyruvate Kinase M2 Inhibits CD4+ T Cell Pathogenicity and Suppresses Autoimmunity. Cell Metab. 2020;31:391-405.e8 pubmed publisher
	Manson J, Hoffman R, Chen S, Ramadan M, Billiar T. Innate-Like Lymphocytes Are Immediate Participants in the Hyper-Acute Immune Response to Trauma and Hemorrhagic Shock. Front Immunol. 2019;10:1501 pubmed publisher
	Levin M, Kroehl M, Johnson M, Hammes A, Reinhold D, Lang N, et al. Th1 memory differentiates recombinant from live herpes zoster vaccines. J Clin Invest. 2018;128:4429-4440 pubmed publisher
	Iwamoto S, Iwai S, Tsujiyama K, Kurahashi C, Takeshita K, Naoe M, et al. TNF-alpha drives human CD14+ monocytes to differentiate into CD70+ dendritic cells evoking Th1 and Th17 responses. J Immunol. 2007;179:1449-57 pubmed

product information

Antigen :

TNF-alpha

Apps. Abbrev. :

ICFC, FC

Cat # :

502944

Clone :

MAb11

Item :

APC/Cyanine7 anti-human TNF-α

Isotype :

Mouse IgG1, κ

Other Names :

Tumor necrosis factor-α, Cachectin, Necrosin, Macrophage cytotoxic factor (MCF), Differentiation inducing factor (DIF), TNFSF2

Size :

100 tests

Price (USD) :

329 USD

Reactivity :

Human

Clonality :

Monoclonal

Host :

Mouse

Conjugate/Tag/Label :

APC_CYANINE7

Immunogen :

E. coli -expressed, recombinant human TNF-α

Application Notes :

ELISA or ELISPOT Detection: The biotinylated MAb11 antibody is useful as the detection antibody in a sandwich ELISA or ELISPOT, when used in conjunction with the purified MAb1 antibody (Cat. No. 502802/502804) as the capture antibody.Flow Cytometry^3,5,6,10: The fluorochrome-labeled MAb11 antibody is useful for intracellular and membrane-bound immunofluorescent staining and flow cytometric analysis to identify TNF-a-producing cells within mixed cell populations.Additional reported applications (for the relevant formats) include: neutralization^1,2, immunohistochemical staining of paraformaldehyde-fixed, saponin-treated frozen tissue sections^4 and acetone-fixed frozen tissue sections^8, immunocytochemistry^7, and immunofluorescence^9. The MAb11 antibody can neutralize the bioactivity of natural or recombinant TNF-a.Note: For testing human TNF-a in serum or plasma, BioLegend's ELISA Max™ Sets (Cat. No. 430201 to 430206) are specially developed and recommended. The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for neutralization of human TNF-a bioactivity (Cat. No. 502922).The Purified MAb1 antibody is useful in neutralization^2 and as the capture antibody in a sandwich ELISA or ELISPOT assay, when used in conjunction with the biotinylated MAb11 antibody (Cat. No. 502904/502914) as the detecting antibody.Clone MAb11 cross-reacts to Cat^11;

more info or order :

BioLegend product webpage