Brilliant Violet 711™ anti-human TNF-α | BioLegend 502940 product information

product summary

company name :

BioLegend

other brands :

Babco, Signet, Sternberger Monoclonals, Senetek, Covance

product type :

antibody

product name :

Brilliant Violet 711™ anti-human TNF-α

catalog :

502940

quantity :

100 tests

price :

446 USD

clonality :

monoclonal

host :

mouse

conjugate :

BV711

clone name :

MAb11

reactivity :

human

application :

immunocytochemistry, flow cytometry

more info or order :

BioLegend product webpage

citations: 12

Published Application/Species/Sample/Dilution	Reference
flow cytometry; human; 1:100; fig 4i	Spiegel J, Patel S, Muffly L, Hossain N, Oak J, Baird J, et al. CAR T cells with dual targeting of CD19 and CD22 in adult patients with recurrent or refractory B cell malignancies: a phase 1 trial. Nat Med. 2021;27:1419-1431 pubmed publisher
flow cytometry; human; loading ...; fig s11g	Weber E, Parker K, Sotillo E, Lynn R, Anbunathan H, Lattin J, et al. Transient rest restores functionality in exhausted CAR-T cells through epigenetic remodeling. Science. 2021;372: pubmed publisher
flow cytometry; human; loading ...; fig 6d	Lynn R, Weber E, Sotillo E, Gennert D, Xu P, Good Z, et al. c-Jun overexpression in CAR T cells induces exhaustion resistance. Nature. 2019;576:293-300 pubmed publisher
	Billing F, Walter B, Fink S, Arefaine E, Pickarski L, Maier S, et al. Altered Proinflammatory Responses to Polyelectrolyte Multilayer Coatings Are Associated with Differences in Protein Adsorption and Wettability. ACS Appl Mater Interfaces. 2021;13:55534-55549 pubmed publisher
	Richert Spuhler L, MAR C, Shinde P, Wu F, Hong T, Greene E, et al. CD101 genetic variants modify regulatory and conventional T cell phenotypes and functions. Cell Rep Med. 2021;2:100322 pubmed publisher
	Sia W, Hey Y, Foo R, Wang L, Leeansyah E. Culture, expansion, and flow-cytometry-based functional analysis of pteropid bat MR1-restricted unconventional T cells. STAR Protoc. 2021;2:100487 pubmed publisher
	Clayton K, Mylvaganam G, Villasmil Ocando A, Stuart H, Maus M, Rashidian M, et al. HIV-infected macrophages resist efficient NK cell-mediated killing while preserving inflammatory cytokine responses. Cell Host Microbe. 2021;29:435-447.e9 pubmed publisher
	Li D, Bradley T, Cain D, Pedroza Pacheco I, Aggelakopoulou M, Parks R, et al. RAB11FIP5-Deficient Mice Exhibit Cytokine-Related Transcriptomic Signatures. Immunohorizons. 2020;4:713-728 pubmed publisher
	Hong J, Reynoso G, Andhey P, Swain A, Turner J, Boon A, et al. An Agonistic Anti-CD137 Antibody Disrupts Lymphoid Follicle Structure and T-Cell-Dependent Antibody Responses. Cell Rep Med. 2020;1: pubmed publisher
	Bradley T, Peppa D, Pedroza Pacheco I, Li D, Cain D, Henao R, et al. RAB11FIP5 Expression and Altered Natural Killer Cell Function Are Associated with Induction of HIV Broadly Neutralizing Antibody Responses. Cell. 2018;175:387-399.e17 pubmed publisher
	Fisher J, Abramowski P, Wisidagamage Don N, Flutter B, Capsomidis A, Cheung G, et al. Avoidance of On-Target Off-Tumor Activation Using a Co-stimulation-Only Chimeric Antigen Receptor. Mol Ther. 2017;25:1234-1247 pubmed publisher
	Iwamoto S, Iwai S, Tsujiyama K, Kurahashi C, Takeshita K, Naoe M, et al. TNF-alpha drives human CD14+ monocytes to differentiate into CD70+ dendritic cells evoking Th1 and Th17 responses. J Immunol. 2007;179:1449-57 pubmed

product information

Antigen :

TNF-alpha

Apps. Abbrev. :

ICFC, FC

Cat # :

502940

Clone :

MAb11

Item :

Brilliant Violet 711™ anti-human TNF-α

Isotype :

Mouse IgG1, κ

Other Names :

Tumor necrosis factor-α, Cachectin, Necrosin, Macrophage cytotoxic factor (MCF), Differentiation inducing factor (DIF), TNFSF2

Size :

100 tests

Price (USD) :

446 USD

Reactivity :

Human

Clonality :

Monoclonal

Host :

Mouse

Conjugate/Tag/Label :

BV711

Immunogen :

E. coli -expressed, recombinant human TNF-α

Application Notes :

ELISA or ELISPOT Detection: The biotinylated MAb11 antibody is useful as the detection antibody in a sandwich ELISA or ELISPOT, when used in conjunction with the purified MAb1 antibody (Cat. No. 502802/502804) as the capture antibody.Flow Cytometry^3,5,6,10: The fluorochrome-labeled MAb11 antibody is useful for intracellular and membrane-bound immunofluorescent staining and flow cytometric analysis to identify TNF-a-producing cells within mixed cell populations.Additional reported applications (for the relevant formats) include: neutralization^1,2, immunohistochemical staining of paraformaldehyde-fixed, saponin-treated frozen tissue sections^4 and acetone-fixed frozen tissue sections^8, immunocytochemistry^7, and immunofluorescence^9. The MAb11 antibody can neutralize the bioactivity of natural or recombinant TNF-a.Note: For testing human TNF-a in serum or plasma, BioLegend's ELISA Max™ Sets (Cat. No. 430201 to 430206) are specially developed and recommended. The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for neutralization of human TNF-a bioactivity (Cat. No. 502922).The Purified MAb1 antibody is useful in neutralization^2 and as the capture antibody in a sandwich ELISA or ELISPOT assay, when used in conjunction with the biotinylated MAb11 antibody (Cat. No. 502904/502914) as the detecting antibody.Clone MAb11 cross-reacts to Cat^11;

more info or order :

BioLegend product webpage