PerCP/Cyanine5.5 anti-human TNF-α | BioLegend 502926 product information

product summary

company name :

BioLegend

other brands :

Babco, Signet, Sternberger Monoclonals, Senetek, Covance

product type :

antibody

product name :

PerCP/Cyanine5.5 anti-human TNF-α

catalog :

502926

quantity :

100 tests

price :

382 USD

clonality :

monoclonal

host :

mouse

conjugate :

PerCP/Cy5.5

clone name :

MAb11

reactivity :

human

application :

immunocytochemistry, flow cytometry

more info or order :

BioLegend product webpage

citations: 16

Published Application/Species/Sample/Dilution	Reference
flow cytometry; human; loading ...; fig 6a	Banki Z, Krabbendam L, Klaver D, Leng T, Kruis S, Mehta H, et al. Antibody opsonization enhances MAIT cell responsiveness to bacteria via a TNF-dependent mechanism. Immunol Cell Biol. 2019;97:538-551 pubmed publisher
	Jiang X, Wang G, Zhao X, Yan F, Yao L, Kou Z, et al. Lasting antibody and T cell responses to SARS-CoV-2 in COVID-19 patients three months after infection. Nat Commun. 2021;12:897 pubmed publisher
	Leng T, Akther H, Hackstein C, Powell K, King T, Friedrich M, et al. TCR and Inflammatory Signals Tune Human MAIT Cells to Exert Specific Tissue Repair and Effector Functions. Cell Rep. 2019;28:3077-3091.e5 pubmed publisher
	de Jonge K, Ebering A, Nassiri S, Maby El Hajjami H, Ouertatani Sakouhi H, Baumgaertner P, et al. Circulating CD56bright NK cells inversely correlate with survival of melanoma patients. Sci Rep. 2019;9:4487 pubmed publisher
	Lee B, Jo Y, Kim G, Ali L, Sohn D, Lee S, et al. Specific Inhibition of Soluble γc Receptor Attenuates Collagen-Induced Arthritis by Modulating the Inflammatory T Cell Responses. Front Immunol. 2019;10:209 pubmed publisher
	Tuero I, Venzon D, Robert Guroff M. Mucosal and Systemic Î³Î´+ T Cells Associated with Control of Simian Immunodeficiency Virus Infection. J Immunol. 2016;197:4686-4695 pubmed
	Sekine T, Marin D, Cao K, Li L, Mehta P, Shaim H, et al. Specific combinations of donor and recipient KIR-HLA genotypes predict for large differences in outcome after cord blood transplantation. Blood. 2016;128:297-312 pubmed publisher
	Glaser K, Fehrholz M, Curstedt T, Kunzmann S, Speer C. Effects of the New Generation Synthetic Reconstituted Surfactant CHF5633 on Pro- and Anti-Inflammatory Cytokine Expression in Native and LPS-Stimulated Adult CD14+ Monocytes. PLoS ONE. 2016;11:e0146898 pubmed publisher
	Bsat M, Chapuy L, Baba N, Rubio M, Panzini B, Wassef R, et al. Differential accumulation and function of proinflammatory 6-sulfo LacNAc dendritic cells in lymph node and colon of Crohn's versus ulcerative colitis patients. J Leukoc Biol. 2015;98:671-81 pubmed publisher
	Perić Z, Cahu X, Malard F, Brissot E, Chevallier P, Guillaume T, et al. Peripheral Blood Plasmacytoid Dendritic Cells at Day 100 Can Predict Outcome after Allogeneic Stem Cell Transplantation. Biol Blood Marrow Transplant. 2015;21:1431-6 pubmed publisher
	Deberge M, Ely K, Cheng G, Enelow R. ADAM17-mediated processing of TNF-α expressed by antiviral effector CD8+ T cells is required for severe T-cell-mediated lung injury. PLoS ONE. 2013;8:e79340 pubmed publisher
	Krishnan S, Wilson E, Sheikh V, Rupert A, Mendoza D, Yang J, et al. Evidence for innate immune system activation in HIV type 1-infected elite controllers. J Infect Dis. 2014;209:931-9 pubmed publisher
	Smits K, Pottier G, Smet J, Dirix V, Vermeulen F, De Schutter I, et al. Different T cell memory in preadolescents after whole-cell or acellular pertussis vaccination. Vaccine. 2013;32:111-8 pubmed publisher
	Vargas Inchaustegui D, Xiao P, Tuero I, Patterson L, Robert Guroff M. NK and CD4+ T cell cooperative immune responses correlate with control of disease in a macaque simian immunodeficiency virus infection model. J Immunol. 2012;189:1878-85 pubmed publisher
	Li X, Miao H, Henn A, Topham D, Wu H, Zand M, et al. Ki-67 expression reveals strong, transient influenza specific CD4 T cell responses after adult vaccination. Vaccine. 2012;30:4581-4 pubmed publisher
	Iwamoto S, Iwai S, Tsujiyama K, Kurahashi C, Takeshita K, Naoe M, et al. TNF-alpha drives human CD14+ monocytes to differentiate into CD70+ dendritic cells evoking Th1 and Th17 responses. J Immunol. 2007;179:1449-57 pubmed

product information

Antigen :

TNF-alpha

Apps. Abbrev. :

ICFC, FC

Cat # :

502926

Clone :

MAb11

Item :

PerCP/Cyanine5.5 anti-human TNF-α

Isotype :

Mouse IgG1, κ

Other Names :

Tumor necrosis factor-α, Cachectin, Necrosin, Macrophage cytotoxic factor (MCF), Differentiation inducing factor (DIF), TNFSF2

Size :

100 tests

Price (USD) :

382 USD

Reactivity :

Human

Clonality :

Monoclonal

Host :

Mouse

Conjugate/Tag/Label :

PERCP_CYANINE5.5

Immunogen :

E. coli -expressed, recombinant human TNF-α

Application Notes :

ELISA or ELISPOT Detection: The biotinylated MAb11 antibody is useful as the detection antibody in a sandwich ELISA or ELISPOT, when used in conjunction with the purified MAb1 antibody (Cat. No. 502802/502804) as the capture antibody.Flow Cytometry^3,5,6,10: The fluorochrome-labeled MAb11 antibody is useful for intracellular and membrane-bound immunofluorescent staining and flow cytometric analysis to identify TNF-a-producing cells within mixed cell populations.Additional reported applications (for the relevant formats) include: neutralization^1,2, immunohistochemical staining of paraformaldehyde-fixed, saponin-treated frozen tissue sections^4 and acetone-fixed frozen tissue sections^8, immunocytochemistry^7, and immunofluorescence^9. The MAb11 antibody can neutralize the bioactivity of natural or recombinant TNF-a.Note: For testing human TNF-a in serum or plasma, BioLegend's ELISA Max™ Sets (Cat. No. 430201 to 430206) are specially developed and recommended. The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for neutralization of human TNF-a bioactivity (Cat. No. 502922).The Purified MAb1 antibody is useful in neutralization^2 and as the capture antibody in a sandwich ELISA or ELISPOT assay, when used in conjunction with the biotinylated MAb11 antibody (Cat. No. 502904/502914) as the detecting antibody.Clone MAb11 cross-reacts to Cat^11;

more info or order :

BioLegend product webpage