Pacific Blue™ anti-human TNF-α | BioLegend 502920 product information

product summary

company name :

BioLegend

other brands :

Babco, Signet, Sternberger Monoclonals, Senetek, Covance

product type :

antibody

product name :

Pacific Blue™ anti-human TNF-α

catalog :

502920

quantity :

100 μg

price :

265 USD

clonality :

monoclonal

host :

mouse

conjugate :

Pacific Blue

clone name :

MAb11

reactivity :

human, rhesus macaque

application :

immunocytochemistry, flow cytometry

more info or order :

BioLegend product webpage

citations: 24

Published Application/Species/Sample/Dilution	Reference
flow cytometry; human; loading ...; fig 5b	Iampietro M, Younan P, Nishida A, Dutta M, Lubaki N, Santos R, et al. Ebola virus glycoprotein directly triggers T lymphocyte death despite of the lack of infection. PLoS Pathog. 2017;13:e1006397 pubmed publisher
flow cytometry; human; loading ...; fig 4c	Nelde A, Walz J, Kowalewski D, Schuster H, Wolz O, Peper J, et al. HLA class I-restricted MYD88 L265P-derived peptides as specific targets for lymphoma immunotherapy. Oncoimmunology. 2017;6:e1219825 pubmed publisher
flow cytometry; human; loading ...; fig 2a	Klinker M, Marklein R, Lo Surdo J, Wei C, Bauer S. Morphological features of IFN-γ-stimulated mesenchymal stromal cells predict overall immunosuppressive capacity. Proc Natl Acad Sci U S A. 2017;114:E2598-E2607 pubmed publisher
flow cytometry; rhesus macaque; loading ...	Vaccari M, Gordon S, Fourati S, Schifanella L, Liyanage N, Cameron M, et al. Adjuvant-dependent innate and adaptive immune signatures of risk of SIVmac251 acquisition. Nat Med. 2016;22:762-70 pubmed publisher
	Liu W. Evaluating Human Natural Killer Cells Antibody-dependent Cellular Cytotoxicity (ADCC) Using Plate-bound Anti-CD16 Antibodies. Bio Protoc. 2022;12:e4285 pubmed publisher
	Hamilton J, Tsuchida C, Nguyen D, Shy B, McGarrigle E, Sandoval Espinoza C, et al. Targeted delivery of CRISPR-Cas9 and transgenes enables complex immune cell engineering. Cell Rep. 2021;35:109207 pubmed publisher
	Bilich T, Nelde A, Heitmann J, Maringer Y, Roerden M, Bauer J, et al. T cell and antibody kinetics delineate SARS-CoV-2 peptides mediating long-term immune responses in COVID-19 convalescent individuals. Sci Transl Med. 2021;13: pubmed publisher
	Baban B, Braun M, Khodadadi H, Ward A, Alverson K, Malik A, et al. AMPK induces regulatory innate lymphoid cells after traumatic brain injury. JCI Insight. 2021;6: pubmed publisher
	Braun J, Loyal L, Frentsch M, Wendisch D, Georg P, Kurth F, et al. SARS-CoV-2-reactive T cells in healthy donors and patients with COVID-19. Nature. 2020;587:270-274 pubmed publisher
	Fox A, Dutt T, Karger B, Obregón Henao A, Anderson G, Henao Tamayo M. Acquisition of High-Quality Spectral Flow Cytometry Data. Curr Protoc Cytom. 2020;93:e74 pubmed publisher
	Ebner F, Morrison E, Bertazzon M, Midha A, Hartmann S, Freund C, et al. CD4+ Th immunogenicity of the Ascaris spp. secreted products. NPJ Vaccines. 2020;5:25 pubmed publisher
	Bilich T, Nelde A, Bauer J, Walz S, Roerden M, Salih H, et al. Mass spectrometry-based identification of a B-cell maturation antigen-derived T-cell epitope for antigen-specific immunotherapy of multiple myeloma. Blood Cancer J. 2020;10:24 pubmed publisher
	Lübke M, Spalt S, Kowalewski D, Zimmermann C, Bauersfeld L, Nelde A, et al. Identification of HCMV-derived T cell epitopes in seropositive individuals through viral deletion models. J Exp Med. 2020;217: pubmed publisher
	Reiske L, Schmucker S, Steuber J, Stefanski V. Glucocorticoids and Catecholamines Affect in Vitro Functionality of Porcine Blood Immune Cells. Animals (Basel). 2019;9: pubmed publisher
	Salvany Celades M, van der Zwan A, Benner M, Setrajcic Dragos V, Bougleux Gomes H, Iyer V, et al. Three Types of Functional Regulatory T Cells Control T Cell Responses at the Human Maternal-Fetal Interface. Cell Rep. 2019;27:2537-2547.e5 pubmed publisher
	Vaccari M, Fourati S, Gordon S, Brown D, Bissa M, Schifanella L, et al. HIV vaccine candidate activation of hypoxia and the inflammasome in CD14+ monocytes is associated with a decreased risk of SIVmac251 acquisition. Nat Med. 2018;24:847-856 pubmed publisher
	Keller A, Eckle S, Xu W, Liu L, Hughes V, Mak J, et al. Drugs and drug-like molecules can modulate the function of mucosal-associated invariant T cells. Nat Immunol. 2017;18:402-411 pubmed publisher
	Morgan S, Hemmink J, Porter E, Harley R, Shelton H, Aramouni M, et al. Aerosol Delivery of a Candidate Universal Influenza Vaccine Reduces Viral Load in Pigs Challenged with Pandemic H1N1 Virus. J Immunol. 2016;196:5014-23 pubmed publisher
	McWilliam H, Eckle S, Theodossis A, Liu L, Chen Z, Wubben J, et al. The intracellular pathway for the presentation of vitamin B-related antigens by the antigen-presenting molecule MR1. Nat Immunol. 2016;17:531-7 pubmed publisher
	Bidmon N, Attig S, Rae R, SchrÃ¶der H, Omokoko T, Simon P, et al. Generation of TCR-engineered T cells and their use to control the performance of T cell assays. J Immunol. 2015;194:6177-89 pubmed publisher
	Canaday D, Sridaran S, Van Epps P, Aung H, Burant C, Nsereko M, et al. CD4+ T cell polyfunctional profile in HIV-TB coinfection are similar between individuals with latent and active TB infection. Tuberculosis (Edinb). 2015;95:470-5 pubmed publisher
	Braun M, BjÃ¶rkstrÃ¶m N, Gupta S, SundstrÃ¶m K, Ahlm C, KlingstrÃ¶m J, et al. NK cell activation in human hantavirus infection explained by virus-induced IL-15/IL15RÎ± expression. PLoS Pathog. 2014;10:e1004521 pubmed publisher
	Franzoni G, Kurkure N, Edgar D, Everett H, Gerner W, Bodman Smith K, et al. Assessment of the phenotype and functionality of porcine CD8 T cell responses following vaccination with live attenuated classical swine fever virus (CSFV) and virulent CSFV challenge. Clin Vaccine Immunol. 2013;20:1604-16 pubmed publisher
	Iwamoto S, Iwai S, Tsujiyama K, Kurahashi C, Takeshita K, Naoe M, et al. TNF-alpha drives human CD14+ monocytes to differentiate into CD70+ dendritic cells evoking Th1 and Th17 responses. J Immunol. 2007;179:1449-57 pubmed

product information

Antigen :

TNF-alpha

Apps. Abbrev. :

ICFC, FC

Cat # :

502920

Clone :

MAb11

Item :

Pacific Blue™ anti-human TNF-α

Isotype :

Mouse IgG1, κ

Other Names :

Tumor necrosis factor-α, Cachectin, Necrosin, Macrophage cytotoxic factor (MCF), Differentiation inducing factor (DIF), TNFSF2

Size :

100 μg

Price (USD) :

265 USD

Reactivity :

Human

Clonality :

Monoclonal

Host :

Mouse

Conjugate/Tag/Label :

Immunogen :

E. coli -expressed, recombinant human TNF-α

Application Notes :

ELISA or ELISPOT Detection: The biotinylated MAb11 antibody is useful as the detection antibody in a sandwich ELISA or ELISPOT, when used in conjunction with the purified MAb1 antibody (Cat. No. 502802/502804) as the capture antibody.Flow Cytometry^3,5,6,10: The fluorochrome-labeled MAb11 antibody is useful for intracellular and membrane-bound immunofluorescent staining and flow cytometric analysis to identify TNF-a-producing cells within mixed cell populations.Additional reported applications (for the relevant formats) include: neutralization^1,2, immunohistochemical staining of paraformaldehyde-fixed, saponin-treated frozen tissue sections^4 and acetone-fixed frozen tissue sections^8, immunocytochemistry^7, and immunofluorescence^9. The MAb11 antibody can neutralize the bioactivity of natural or recombinant TNF-a.Note: For testing human TNF-a in serum or plasma, BioLegend's ELISA Max™ Sets (Cat. No. 430201 to 430206) are specially developed and recommended. The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for neutralization of human TNF-a bioactivity (Cat. No. 502922).The Purified MAb1 antibody is useful in neutralization^2 and as the capture antibody in a sandwich ELISA or ELISPOT assay, when used in conjunction with the biotinylated MAb11 antibody (Cat. No. 502904/502914) as the detecting antibody.Clone MAb11 cross-reacts to Cat^11;

more info or order :

BioLegend product webpage