APC anti-human TNF-α | BioLegend 502912 product information

product summary

company name :

BioLegend

other brands :

Babco, Signet, Sternberger Monoclonals, Senetek, Covance

product type :

antibody

product name :

APC anti-human TNF-α

catalog :

502912

quantity :

100 tests

price :

279 USD

clonality :

monoclonal

host :

mouse

conjugate :

APC

clone name :

MAb11

reactivity :

human

application :

immunocytochemistry, flow cytometry

more info or order :

BioLegend product webpage

citations: 22

Published Application/Species/Sample/Dilution	Reference
flow cytometry; human; loading ...; fig 7c	Snyder M, Sembrat J, Noda K, MYERBURG M, Craig A, Mitash N, et al. Human Lung-Resident Macrophages Colocalize with and Provide Costimulation to PD1hi Tissue-Resident Memory T Cells. Am J Respir Crit Care Med. 2021;203:1230-1244 pubmed publisher
flow cytometry; human; fig s1e	Cheng W, van Asten S, Burns L, Evans H, Walter G, Hashim A, et al. Periodontitis-associated pathogens P. gingivalis and A. actinomycetemcomitans activate human CD14(+) monocytes leading to enhanced Th17/IL-17 responses. Eur J Immunol. 2016;46:2211-21 pubmed publisher
flow cytometry; human; loading ...; fig 6a	Gadd V, Patel P, Jose S, Horsfall L, Powell E, Irvine K. Altered Peripheral Blood Monocyte Phenotype and Function in Chronic Liver Disease: Implications for Hepatic Recruitment and Systemic Inflammation. PLoS ONE. 2016;11:e0157771 pubmed publisher
	Gussarow D, Bonifacius A, Cossmann A, Stankov M, Mausberg P, Tischer Zimmermann S, et al. Long-Lasting Immunity Against SARS-CoV-2: Dream or Reality?. Front Med (Lausanne). 2021;8:770381 pubmed publisher
	Cai J, Gao H, Zhao J, Hu S, Liang X, Yang Y, et al. Infection with a newly designed dual fluorescent reporter HIV-1 effectively identifies latently infected CD4+ T cells. elife. 2021;10: pubmed publisher
	Bonifacius A, Tischer Zimmermann S, Dragon A, Gussarow D, Vogel A, Krettek U, et al. COVID-19 immune signatures reveal stable antiviral T cell function despite declining humoral responses. Immunity. 2021;54:340-354.e6 pubmed publisher
	Orrantia A, Terrén I, Vitallé J, Astarloa Pando G, Zenarruzabeitia O, Borrego F. Identification and Functional Analysis of Human CD56neg NK Cells by Flow Cytometry. STAR Protoc. 2020;1:100149 pubmed publisher
	Ni J, Wang X, Stojanovic A, Zhang Q, Wincher M, Bühler L, et al. Single-Cell RNA Sequencing of Tumor-Infiltrating NK Cells Reveals that Inhibition of Transcription Factor HIF-1α Unleashes NK Cell Activity. Immunity. 2020;52:1075-1087.e8 pubmed publisher
	Abd Hamid M, Yao X, Waugh C, Rosendo Machado S, Li C, Rostron T, et al. Defective Interferon Gamma Production by Tumor-Specific CD8+ T Cells Is Associated With 5'Methylcytosine-Guanine Hypermethylation of Interferon Gamma Promoter. Front Immunol. 2020;11:310 pubmed publisher
	Tang X, Zhang S, Peng Q, Ling L, Shi H, Liu Y, et al. Sustained IFN-I stimulation impairs MAIT cell responses to bacteria by inducing IL-10 during chronic HIV-1 infection. Sci Adv. 2020;6:eaaz0374 pubmed publisher
	Sánchez N, Medrano Jiménez E, Aguilar Leon D, Pérez Martínez L, Pedraza Alva G. Tumor Necrosis Factor-Induced miR-146a Upregulation Promotes Human Lung Adenocarcinoma Metastasis by Targeting Merlin. DNA Cell Biol. 2020;39:484-497 pubmed publisher
	Zhu Y, Smith D, Zhou Y, Li Y, Yu J, Lee D, et al. Development of Hematopoietic Stem Cell-Engineered Invariant Natural Killer T Cell Therapy for Cancer. Cell Stem Cell. 2019;25:542-557.e9 pubmed publisher
	Abd Hamid M, Wang R, Yao X, Fan P, Li X, Chang X, et al. Enriched HLA-E and CD94/NKG2A interaction limits antitumor CD8+ tumor-infiltrating T lymphocyte responses. Cancer Immunol Res. 2019;: pubmed publisher
	Yu J, Zhang H, Sun S, Sun S, Li L. The effects of Tim-3 activation on T-cells in gastric cancer progression. Oncol Lett. 2019;17:1461-1466 pubmed publisher
	Sun Z, Chang B, Huang A, Hao S, Gao M, Sun Y, et al. Plasma levels of soluble ST2, but not IL-33, correlate with the severity of alcoholic liver disease. J Cell Mol Med. 2019;23:887-897 pubmed publisher
	Liu Z, Dang E, Li B, Qiao H, Jin L, Zhang J, et al. Dysfunction of CD19+CD24hiCD27+ B regulatory cells in patients with bullous pemphigoid. Sci Rep. 2018;8:703 pubmed publisher
	Vitallé J, Zenarruzabeitia O, Terrén I, Plana M, Guardo A, Leal L, et al. Monocytes Phenotype and Cytokine Production in Human Immunodeficiency Virus-1 Infected Patients Receiving a Modified Vaccinia Ankara-Based HIV-1 Vaccine: Relationship to CD300 Molecules Expression. Front Immunol. 2017;8:836 pubmed publisher
	Vacaflores A, Freedman S, Chapman N, Houtman J. Pretreatment of activated human CD8 T cells with IL-12 leads to enhanced TCR-induced signaling and cytokine production. Mol Immunol. 2017;81:1-15 pubmed publisher
	Cuff A, Robertson F, Stegmann K, Pallett L, Maini M, Davidson B, et al. Eomeshi NK Cells in Human Liver Are Long-Lived and Do Not Recirculate but Can Be Replenished from the Circulation. J Immunol. 2016;197:4283-4291 pubmed
	Bsat M, Chapuy L, Baba N, Rubio M, Panzini B, Wassef R, et al. Differential accumulation and function of proinflammatory 6-sulfo LacNAc dendritic cells in lymph node and colon of Crohn's versus ulcerative colitis patients. J Leukoc Biol. 2015;98:671-81 pubmed publisher
	Steindor M, Nkwouano V, Mayatepek E, Mackenzie C, Schramm D, Jacobsen M. Rapid detection and immune characterization of Mycobacterium abscessus infection in cystic fibrosis patients. PLoS ONE. 2015;10:e0119737 pubmed publisher
	Schmidt E, Linz B, Diekelmann S, Besedovsky L, Lange T, Born J. Effects of an interleukin-1 receptor antagonist on human sleep, sleep-associated memory consolidation, and blood monocytes. Brain Behav Immun. 2015;47:178-85 pubmed publisher

product information

Antigen :

TNF-alpha

Apps. Abbrev. :

ICFC, FC

Cat # :

502912

Clone :

MAb11

Item :

APC anti-human TNF-α

Isotype :

Mouse IgG1, κ

Other Names :

Tumor necrosis factor-α, Cachectin, Necrosin, Macrophage cytotoxic factor (MCF), Differentiation inducing factor (DIF), TNFSF2

Size :

100 tests

Price (USD) :

279 USD

Reactivity :

Human

Clonality :

Monoclonal

Host :

Mouse

Conjugate/Tag/Label :

APC

Immunogen :

E. coli -expressed, recombinant human TNF-α

Application Notes :

ELISA or ELISPOT Detection: The biotinylated MAb11 antibody is useful as the detection antibody in a sandwich ELISA or ELISPOT, when used in conjunction with the purified MAb1 antibody (Cat. No. 502802/502804) as the capture antibody.Flow Cytometry^3,5,6,10: The fluorochrome-labeled MAb11 antibody is useful for intracellular and membrane-bound immunofluorescent staining and flow cytometric analysis to identify TNF-a-producing cells within mixed cell populations.Additional reported applications (for the relevant formats) include: neutralization^1,2, immunohistochemical staining of paraformaldehyde-fixed, saponin-treated frozen tissue sections^4 and acetone-fixed frozen tissue sections^8, immunocytochemistry^7, and immunofluorescence^9. The MAb11 antibody can neutralize the bioactivity of natural or recombinant TNF-a.Note: For testing human TNF-a in serum or plasma, BioLegend's ELISA Max™ Sets (Cat. No. 430201 to 430206) are specially developed and recommended. The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for neutralization of human TNF-a bioactivity (Cat. No. 502922).The Purified MAb1 antibody is useful in neutralization^2 and as the capture antibody in a sandwich ELISA or ELISPOT assay, when used in conjunction with the biotinylated MAb11 antibody (Cat. No. 502904/502914) as the detecting antibody.Clone MAb11 cross-reacts to Cat^11;

more info or order :

BioLegend product webpage