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company name :
BioLegend
other brands :
Babco, Signet, Sternberger Monoclonals, Senetek, Covance
product type :
antibody
product name :
PE anti-human TNF-α
catalog :
502909
quantity :
100 tests
price :
244 USD
clonality :
monoclonal
host :
mouse
conjugate :
PE
clone name :
MAb11
reactivity :
human
application :
immunocytochemistry, flow cytometry
more info or order :
citations: 32
Published Application/Species/Sample/DilutionReference
  • flow cytometry; human; 1:20; loading ...; fig 6h
Zinngrebe J, Moepps B, Monecke T, Gierschik P, Schlichtig F, Barth T, et al. Compound heterozygous variants in OTULIN are associated with fulminant atypical late-onset ORAS. EMBO Mol Med. 2022;14:e14901 pubmed publisher
  • flow cytometry; human; 3:50; loading ...; fig 4a
Gregorova M, Morse D, Brignoli T, Steventon J, Hamilton F, Albur M, et al. Post-acute COVID-19 associated with evidence of bystander T-cell activation and a recurring antibiotic-resistant bacterial pneumonia. elife. 2020;9: pubmed publisher
  • flow cytometry; human; loading ...; fig 4d
Oh D, Kwek S, Raju S, Li T, McCarthy E, Chow E, et al. Intratumoral CD4+ T Cells Mediate Anti-tumor Cytotoxicity in Human Bladder Cancer. Cell. 2020;181:1612-1625.e13 pubmed publisher
  • flow cytometry; human; loading ...; fig s7d
Wu J, Ma S, Sandhoff R, Ming Y, Hotz Wagenblatt A, Timmerman V, et al. Loss of Neurological Disease HSAN-I-Associated Gene SPTLC2 Impairs CD8+ T Cell Responses to Infection by Inhibiting T Cell Metabolic Fitness. Immunity. 2019;50:1218-1231.e5 pubmed publisher
  • flow cytometry; human; loading ...; fig 3a
Kuranda K, Jean Alphonse P, Leborgne C, Hardet R, Collaud F, Marmier S, et al. Exposure to wild-type AAV drives distinct capsid immunity profiles in humans. J Clin Invest. 2018;128:5267-5279 pubmed publisher
  • flow cytometry; human; loading ...; fig 3b
Li N, van Unen V, Höllt T, Thompson A, van Bergen J, Pezzotti N, et al. Mass cytometry reveals innate lymphoid cell differentiation pathways in the human fetal intestine. J Exp Med. 2018;215:1383-1396 pubmed publisher
  • flow cytometry; human; loading ...; fig 3a
Duggal N, Beswetherick A, Upton J, Hampson P, Phillips A, Lord J. Depressive symptoms in hip fracture patients are associated with reduced monocyte superoxide production. Exp Gerontol. 2014;54:27-34 pubmed publisher
Pi J, Zhang Z, Yang E, Chen L, Zeng L, Chen Y, et al. Nanocages engineered from Bacillus Calmette-Guerin facilitate protective Vγ2Vδ2 T cell immunity against Mycobacterium tuberculosis infection. J Nanobiotechnology. 2022;20:36 pubmed publisher
Rice T, Diavatopoulos D, Guo Y, Donaldson B, Bouqueau M, Bosanquet A, et al. Modification of innate immune responses to Bordetella pertussis in babies from pertussis vaccinated pregnancies. EBioMedicine. 2021;72:103612 pubmed publisher
Schmidt S, Kreutzmann H, Stadler M, Mair K, Stas M, Koch M, et al. T-Cell Cytokine Response in Salmonella Typhimurium-Vaccinated versus Infected Pigs. Vaccines (Basel). 2021;9: pubmed publisher
Loo Yau H, De Carvalho D. Measuring the effect of drug treatments on primary human CD8+ T cell activation and cytolytic potential. STAR Protoc. 2021;2:100549 pubmed publisher
Ma X, Xiao L, Liu L, Ye L, Su P, Bi E, et al. CD36-mediated ferroptosis dampens intratumoral CD8+ T cell effector function and impairs their antitumor ability. Cell Metab. 2021;33:1001-1012.e5 pubmed publisher
Loo Yau H, Bell E, Ettayebi I, de Almeida F, Boukhaled G, Shen S, et al. DNA hypomethylating agents increase activation and cytolytic activity of CD8+ T cells. Mol Cell. 2021;81:1469-1483.e8 pubmed publisher
Fang Y, Wang L, Wan C, Sun Y, Van der Jeught K, Zhou Z, et al. MAL2 drives immune evasion in breast cancer by suppressing tumor antigen presentation. J Clin Invest. 2021;131: pubmed publisher
Roudko V, Bozkus C, Orfanelli T, McClain C, Carr C, O Donnell T, et al. Shared Immunogenic Poly-Epitope Frameshift Mutations in Microsatellite Unstable Tumors. Cell. 2020;183:1634-1649.e17 pubmed publisher
Jiang G, Shi L, Zheng X, Zhang X, Wu K, Liu B, et al. Androgen receptor affects the response to immune checkpoint therapy by suppressing PD-L1 in hepatocellular carcinoma. Aging (Albany NY). 2020;12:11466-11484 pubmed publisher
Zhu X, Liu H, Zhang Z, Wei R, Zhou X, Wang Z, et al. MiR-103 protects from recurrent spontaneous abortion via inhibiting STAT1 mediated M1 macrophage polarization. Int J Biol Sci. 2020;16:2248-2264 pubmed publisher
Chang Z, Hou A, Chen Y. Engineering primary T cells with chimeric antigen receptors for rewired responses to soluble ligands. Nat Protoc. 2020;15:1507-1524 pubmed publisher
Pham T, Meziane O, Miah M, Volodina O, Colas C, Beland K, et al. Flt3L-Mediated Expansion of Plasmacytoid Dendritic Cells Suppresses HIV Infection in Humanized Mice. Cell Rep. 2019;29:2770-2782.e5 pubmed publisher
Carestia A, Mena H, Olexen C, Ortiz Wilczyñski J, Negrotto S, Errasti A, et al. Platelets Promote Macrophage Polarization toward Pro-inflammatory Phenotype and Increase Survival of Septic Mice. Cell Rep. 2019;28:896-908.e5 pubmed publisher
Ryzhov S, May T, Dziodzio J, Emery I, Lucas F, Leclerc A, et al. Number of Circulating CD 73-Expressing Lymphocytes Correlates With Survival After Cardiac Arrest. J Am Heart Assoc. 2019;8:e010874 pubmed publisher
Picard E, Godet Y, Laheurte C, Dosset M, Galaine J, Beziaud L, et al. Circulating NKp46+ Natural Killer cells have a potential regulatory property and predict distinct survival in Non-Small Cell Lung Cancer. Oncoimmunology. 2019;8:e1527498 pubmed publisher
Kagoya Y, Nakatsugawa M, Saso K, Guo T, Anczurowski M, Wang C, et al. DOT1L inhibition attenuates graft-versus-host disease by allogeneic T cells in adoptive immunotherapy models. Nat Commun. 2018;9:1915 pubmed publisher
Chen D, Xie J, Fiskesund R, Dong W, Liang X, Lv J, et al. Chloroquine modulates antitumor immune response by resetting tumor-associated macrophages toward M1 phenotype. Nat Commun. 2018;9:873 pubmed publisher
Kagoya Y, Tanaka S, Guo T, Anczurowski M, Wang C, Saso K, et al. A novel chimeric antigen receptor containing a JAK-STAT signaling domain mediates superior antitumor effects. Nat Med. 2018;24:352-359 pubmed publisher
Guimarães F, Melo G, Cordeiro T, Feracin V, Vieira E, Pereira W, et al. T-lymphocyte-expressing inflammatory cytokines underlie persistence of proteinuria in children with idiopathic nephrotic syndrome. J Pediatr (Rio J). 2018;94:546-553 pubmed publisher
Almeida V, Avelar Freitas B, Santos M, Costa L, Silva T, Pereira W, et al. Inhibitory effect of the Pseudobrickellia brasiliensis (Spreng) R.M. King & H. Rob. aqueous extract on human lymphocyte proliferation and IFN-? and TNF-? production in vitro. Braz J Med Biol Res. 2017;50:e5163 pubmed publisher
Kessler B, Rinchai D, Kewcharoenwong C, Nithichanon A, Biggart R, Hawrylowicz C, et al. Interleukin 10 inhibits pro-inflammatory cytokine responses and killing of Burkholderia pseudomallei. Sci Rep. 2017;7:42791 pubmed publisher
Chruewkamlow N, Mahasongkram K, Pata S, Chaiwarith R, Salee P, Supparatpinyo K, et al. Immune Alterations in Patients with Anti-Interferon-γ Autoantibodies. PLoS ONE. 2016;11:e0145983 pubmed publisher
Liu B, Ma Y, Zhang Y, Zhang C, Yi J, Zhuang R, et al. CD8low CD100- T Cells Identify a Novel CD8 T Cell Subset Associated with Viral Control during Human Hantaan Virus Infection. J Virol. 2015;89:11834-44 pubmed publisher
Liu Y, Bezverbnaya K, Zhao T, Parsons M, Shi M, Treanor B, et al. Involvement of the HCK and FGR src-family kinases in FCRL4-mediated immune regulation. J Immunol. 2015;194:5851-60 pubmed publisher
Rochman Y, Yukawa M, Kartashov A, Barski A. Functional characterization of human T cell hyporesponsiveness induced by CTLA4-Ig. PLoS ONE. 2015;10:e0122198 pubmed publisher
product information
Antigen :
TNF-alpha
Apps. Abbrev. :
ICFC, FC
Cat # :
502909
Clone :
MAb11
Item :
PE anti-human TNF-α
Isotype :
Mouse IgG1, κ
Other Names :
Tumor necrosis factor-α, Cachectin, Necrosin, Macrophage cytotoxic factor (MCF), Differentiation inducing factor (DIF), TNFSF2
Size :
100 tests
Price (USD) :
244 USD
Reactivity :
Human
Clonality :
Monoclonal
Host :
Mouse
Conjugate/Tag/Label :
PE
Immunogen :
E. coli -expressed, recombinant human TNF-α
Application Notes :
ELISA or ELISPOT Detection: The biotinylated MAb11 antibody is useful as the detection antibody in a sandwich ELISA or ELISPOT, when used in conjunction with the purified MAb1 antibody (Cat. No. 502802/502804) as the capture antibody.Flow Cytometry^3,5,6,10: The fluorochrome-labeled MAb11 antibody is useful for intracellular and membrane-bound immunofluorescent staining and flow cytometric analysis to identify TNF-a-producing cells within mixed cell populations.Additional reported applications (for the relevant formats) include: neutralization^1,2, immunohistochemical staining of paraformaldehyde-fixed, saponin-treated frozen tissue sections^4 and acetone-fixed frozen tissue sections^8, immunocytochemistry^7, and immunofluorescence^9. The MAb11 antibody can neutralize the bioactivity of natural or recombinant TNF-a.Note: For testing human TNF-a in serum or plasma, BioLegend's ELISA Max™ Sets (Cat. No. 430201 to 430206) are specially developed and recommended. The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for neutralization of human TNF-a bioactivity (Cat. No. 502922).The Purified MAb1 antibody is useful in neutralization^2 and as the capture antibody in a sandwich ELISA or ELISPOT assay, when used in conjunction with the biotinylated MAb11 antibody (Cat. No. 502904/502914) as the detecting antibody.Clone MAb11 cross-reacts to Cat^11;
more info or order :
company information
BioLegend
8999 BioLegend Way
San Diego, CA 92121
customerserv@biolegend.com
https://www.biolegend.com
headquarters: USA