Brilliant Violet 650™ anti-human IFN-γ | BioLegend 502538 product information

product summary

company name :

BioLegend

other brands :

Babco, Signet, Sternberger Monoclonals, Senetek, Covance

product type :

antibody

product name :

Brilliant Violet 650™ anti-human IFN-γ

catalog :

502538

quantity :

100 tests

price :

446 USD

clonality :

monoclonal

host :

mouse

conjugate :

BV650

clone name :

4S.B3

reactivity :

human

application :

immunocytochemistry, flow cytometry

more info or order :

BioLegend product webpage

citations: 8

Published Application/Species/Sample/Dilution	Reference
flow cytometry; human; 2 ul/test	Bowcutt R, Malter L, Chen L, Wolff M, Robertson I, Rifkin D, et al. Isolation and cytokine analysis of lamina propria lymphocytes from mucosal biopsies of the human colon. J Immunol Methods. 2015;421:27-35 pubmed publisher
	Holokai L, Chakrabarti J, Lundy J, Croagh D, Adhikary P, Richards S, et al. Murine- and Human-Derived Autologous Organoid/Immune Cell Co-Cultures as Pre-Clinical Models of Pancreatic Ductal Adenocarcinoma. Cancers (Basel). 2020;12: pubmed publisher
	Thieme C, Anft M, Paniskaki K, Blazquez Navarro A, Doevelaar A, Seibert F, et al. Robust T Cell Response Toward Spike, Membrane, and Nucleocapsid SARS-CoV-2 Proteins Is Not Associated with Recovery in Critical COVID-19 Patients. Cell Rep Med. 2020;1:100092 pubmed publisher
	Sparber F, De Gregorio C, Steckholzer S, Ferreira F, Dolowschiak T, Ruchti F, et al. The Skin Commensal Yeast Malassezia Triggers a Type 17 Response that Coordinates Anti-fungal Immunity and Exacerbates Skin Inflammation. Cell Host Microbe. 2019;25:389-403.e6 pubmed publisher
	Koday M, Leonard J, MUNSON P, Forero A, Koday M, Bratt D, et al. Multigenic DNA vaccine induces protective cross-reactive T cell responses against heterologous influenza virus in nonhuman primates. PLoS ONE. 2017;12:e0189780 pubmed publisher
	Nobs S, Natali S, Pohlmeier L, Okreglicka K, Schneider C, Kurrer M, et al. PPARÎ³ in dendritic cells and T cells drives pathogenic type-2 effector responses in lung inflammation. J Exp Med. 2017;214:3015-3035 pubmed publisher
	Payne T, Blackinton J, Frisbee A, Pickeral J, Sawant S, Vandergrift N, et al. Transcriptional and posttranscriptional regulation of cytokine gene expression in HIV-1 antigen-specific CD8+ T cells that mediate virus inhibition. J Virol. 2014;88:9514-28 pubmed publisher
	Iwamoto S, Iwai S, Tsujiyama K, Kurahashi C, Takeshita K, Naoe M, et al. TNF-alpha drives human CD14+ monocytes to differentiate into CD70+ dendritic cells evoking Th1 and Th17 responses. J Immunol. 2007;179:1449-57 pubmed

product information

Antigen :

IFN-gamma

Apps. Abbrev. :

ICFC

Cat # :

502538

Clone :

4S.B3

Item :

Brilliant Violet 650™ anti-human IFN-γ

Isotype :

Mouse IgG1, κ

Other Names :

Interferon-γ, Immune interferon, Type II interferon, T cell interferon, Macrophage-activating factor (MAF), IFN-g, IFN-gamma

Size :

100 tests

Price (USD) :

446 USD

Reactivity :

Human

Clonality :

Monoclonal

Host :

Mouse

Conjugate/Tag/Label :

BV650

Immunogen :

Partially purified, native human IFN-γ

Application Notes :

ELISA or ELISPOT Detection^5: The biotinylated 4S.B3 antibody is useful as a detection antibody for a sandwich ELISA or ELISPOT assay, when used in conjunction with purified NIB42 antibody (Cat. No. 502402/502404) or purified MD-1 antibody (Cat. No. 507502/507513) as the capture antibody.Flow Cytometry^3,4,6-8: The fluorochrome-labeled 4S.B3 antibody is useful for intracellular immunofluorescent staining and flow cytometric analysis to identify IFN-γ -producing cells within mixed cell populations.Additional reported applications (for the relevant formats) include: neutralization^1,2, Western blotting, immunohistochemical staining of paraformaldehyde-fixed, saponin-treated tissue sections, and immunocytochemistry. The 4S.B3 antibody can neutralize the bioactivity of natural or recombinant IFN-γ.Note: For testing human IFN-γ in serum or plasma, BioLegend's ELISA Max™ Sets (Cat. No. 430101 to 430106) are specially developed and recommended.;

more info or order :

BioLegend product webpage