PE/Cyanine7 anti-human IFN-γ | BioLegend 502527 product information

product summary

company name :

BioLegend

other brands :

Babco, Signet, Sternberger Monoclonals, Senetek, Covance

product type :

antibody

product name :

PE/Cyanine7 anti-human IFN-γ

catalog :

502527

quantity :

25 tests

price :

141 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

4S.B3

reactivity :

human

application :

immunocytochemistry, flow cytometry

more info or order :

BioLegend product webpage

citations: 21

Published Application/Species/Sample/Dilution	Reference
flow cytometry; human; loading ...; fig 6g	Secchiari F, Nu xf1 ez S, Sierra J, Ziblat A, Regge M, Raffo Iraolagoitia X, et al. The MICA-NKG2D axis in clear cell renal cell carcinoma bolsters MICA as target in immuno-oncology. Oncoimmunology. 2022;11:2104991 pubmed publisher
flow cytometry; human; loading ...; fig 4i	Hickman T, Choi E, Whiteman K, Muralidharan S, Pai T, Johnson T, et al. BOXR1030, an anti-GPC3 CAR with exogenous GOT2 expression, shows enhanced T cell metabolism and improved anti-cell line derived tumor xenograft activity. PLoS ONE. 2022;17:e0266980 pubmed publisher
flow cytometry; human; fig s10	Kono M, Komatsuda H, Yamaki H, Kumai T, Hayashi R, Wakisaka R, et al. Immunomodulation via FGFR inhibition augments FGFR1 targeting T-cell based antitumor immunotherapy for head and neck squamous cell carcinoma. Oncoimmunology. 2022;11:2021619 pubmed publisher
	Shuwa H, Shaw T, Knight S, Wemyss K, McClure F, Pearmain L, et al. Alterations in T and B cell function persist in convalescent COVID-19 patients. Med (N Y). 2021;2:720-735.e4 pubmed publisher
	Wang J, Xu Y, Chen Z, Liang J, Lin Z, Liang H, et al. Liver Immune Profiling Reveals Pathogenesis and Therapeutics for Biliary Atresia. Cell. 2020;183:1867-1883.e26 pubmed publisher
	Trivedi S, Labuz D, Anderson C, Araújo C, Blair A, Middleton E, et al. Mucosal-associated invariant T (MAIT) cells mediate protective host responses in sepsis. elife. 2020;9: pubmed publisher
	Ping Y, Li F, Nan S, Zhang D, Shi X, Shan J, et al. Augmenting the Effectiveness of CAR-T Cells by Enhanced Self-Delivery of PD-1-Neutralizing scFv. Front Cell Dev Biol. 2020;8:803 pubmed publisher
	Luo X, Cui H, Cai L, Zhu W, Yang W, PATRICK M, et al. Selection of a Clinical Lead TCR Targeting Alpha-Fetoprotein-Positive Liver Cancer Based on a Balance of Risk and Benefit. Front Immunol. 2020;11:623 pubmed publisher
	Fu S, He K, Tian C, Sun H, Zhu C, Bai S, et al. Impaired lipid biosynthesis hinders anti-tumor efficacy of intratumoral iNKT cells. Nat Commun. 2020;11:438 pubmed publisher
	Leng T, Akther H, Hackstein C, Powell K, King T, Friedrich M, et al. TCR and Inflammatory Signals Tune Human MAIT Cells to Exert Specific Tissue Repair and Effector Functions. Cell Rep. 2019;28:3077-3091.e5 pubmed publisher
	Wu J, Ma S, Sandhoff R, Ming Y, Hotz Wagenblatt A, Timmerman V, et al. Loss of Neurological Disease HSAN-I-Associated Gene SPTLC2 Impairs CD8+ T Cell Responses to Infection by Inhibiting T Cell Metabolic Fitness. Immunity. 2019;50:1218-1231.e5 pubmed publisher
	Walk J, de Bree L, Graumans W, Stoter R, van Gemert G, van de Vegte Bolmer M, et al. Outcomes of controlled human malaria infection after BCG vaccination. Nat Commun. 2019;10:874 pubmed publisher
	Kagoya Y, Nakatsugawa M, Saso K, Guo T, Anczurowski M, Wang C, et al. DOT1L inhibition attenuates graft-versus-host disease by allogeneic T cells in adoptive immunotherapy models. Nat Commun. 2018;9:1915 pubmed publisher
	Kagoya Y, Tanaka S, Guo T, Anczurowski M, Wang C, Saso K, et al. A novel chimeric antigen receptor containing a JAK-STAT signaling domain mediates superior antitumor effects. Nat Med. 2018;24:352-359 pubmed publisher
	Chen J, Chernatynskaya A, Li J, Kimbrell M, Cassidy R, Perry D, et al. T cells display mitochondria hyperpolarization in human type 1 diabetes. Sci Rep. 2017;7:10835 pubmed publisher
	Jiang J, Liu C, Liu M, Shen Y, Hu X, Wang Q, et al. OX40 signaling is involved in the autoactivation of CD4+CD28- T cells and contributes to the pathogenesis of autoimmune arthritis. Arthritis Res Ther. 2017;19:67 pubmed publisher
	Bastiaens G, van Meer M, Scholzen A, Obiero J, Vatanshenassan M, van Grinsven T, et al. Safety, Immunogenicity, and Protective Efficacy of Intradermal Immunization with Aseptic, Purified, Cryopreserved Plasmodium falciparum Sporozoites in Volunteers Under Chloroquine Prophylaxis: A Randomized Controlled Trial. Am J Trop Med Hyg. 2016;94:663-73 pubmed publisher
	Fuhrman C, Yeh W, Seay H, Saikumar Lakshmi P, Chopra G, Zhang L, et al. Divergent Phenotypes of Human Regulatory T Cells Expressing the Receptors TIGIT and CD226. J Immunol. 2015;195:145-55 pubmed publisher
	Lim D, Yawata N, Selva K, Li N, Tsai C, Yeong L, et al. The combination of type I IFN, TNF-Î±, and cell surface receptor engagement with dendritic cells enables NK cells to overcome immune evasion by dengue virus. J Immunol. 2014;193:5065-75 pubmed publisher
	Jiang B, Wu X, Li X, Yang X, Zhou Y, Yan H, et al. Expansion of NK cells by engineered K562 cells co-expressing 4-1BBL and mMICA, combined with soluble IL-21. Cell Immunol. 2014;290:10-20 pubmed publisher
	Brembilla N, Ramirez J, Chicheportiche R, Sorg O, Saurat J, Chizzolini C. In vivo dioxin favors interleukin-22 production by human CD4+ T cells in an aryl hydrocarbon receptor (AhR)-dependent manner. PLoS ONE. 2011;6:e18741 pubmed publisher

product information

Antigen :

IFN-gamma

Apps. Abbrev. :

ICFC

Cat # :

502527

Clone :

4S.B3

Item :

PE/Cyanine7 anti-human IFN-γ

Isotype :

Mouse IgG1, κ

Other Names :

Interferon-γ, Immune interferon, Type II interferon, T cell interferon, Macrophage-activating factor (MAF), IFN-g, IFN-gamma

Size :

25 tests

Price (USD) :

141 USD

Reactivity :

Human

Clonality :

Monoclonal

Host :

Mouse

Conjugate/Tag/Label :

PE_CYANINE7

Immunogen :

Partially purified, native human IFN-γ

Application Notes :

ELISA or ELISPOT Detection^5: The biotinylated 4S.B3 antibody is useful as a detection antibody for a sandwich ELISA or ELISPOT assay, when used in conjunction with purified NIB42 antibody (Cat. No. 502402/502404) or purified MD-1 antibody (Cat. No. 507502/507513) as the capture antibody.Flow Cytometry^3,4,6-8: The fluorochrome-labeled 4S.B3 antibody is useful for intracellular immunofluorescent staining and flow cytometric analysis to identify IFN-γ -producing cells within mixed cell populations.Additional reported applications (for the relevant formats) include: neutralization^1,2, Western blotting, immunohistochemical staining of paraformaldehyde-fixed, saponin-treated tissue sections, and immunocytochemistry. The 4S.B3 antibody can neutralize the bioactivity of natural or recombinant IFN-γ.Note: For testing human IFN-γ in serum or plasma, BioLegend's ELISA Max™ Sets (Cat. No. 430101 to 430106) are specially developed and recommended.;

more info or order :

BioLegend product webpage