PerCP/Cyanine5.5 anti-human IFN-γ | BioLegend 502526 product information

product summary

company name :

BioLegend

other brands :

Babco, Signet, Sternberger Monoclonals, Senetek, Covance

product type :

antibody

product name :

PerCP/Cyanine5.5 anti-human IFN-γ

catalog :

502526

quantity :

100 tests

price :

382 USD

clonality :

monoclonal

host :

mouse

conjugate :

PerCP/Cy5.5

clone name :

4S.B3

reactivity :

human

application :

immunocytochemistry, flow cytometry

more info or order :

BioLegend product webpage

citations: 13

Published Application/Species/Sample/Dilution	Reference
flow cytometry; human; loading ...; fig 2c	Wen Z, Jin K, Shen Y, Yang Z, Li Y, Wu B, et al. N-myristoyltransferase deficiency impairs activation of kinase AMPK and promotes synovial tissue inflammation. Nat Immunol. 2019;20:313-325 pubmed publisher
flow cytometry; human; loading ...; fig 1a	Banki Z, Krabbendam L, Klaver D, Leng T, Kruis S, Mehta H, et al. Antibody opsonization enhances MAIT cell responsiveness to bacteria via a TNF-dependent mechanism. Immunol Cell Biol. 2019;97:538-551 pubmed publisher
flow cytometry; human; fig s1d	Cheng W, van Asten S, Burns L, Evans H, Walter G, Hashim A, et al. Periodontitis-associated pathogens P. gingivalis and A. actinomycetemcomitans activate human CD14(+) monocytes leading to enhanced Th17/IL-17 responses. Eur J Immunol. 2016;46:2211-21 pubmed publisher
	Yang R, Mele F, Worley L, Langlais D, Rosain J, Benhsaien I, et al. Human T-bet Governs Innate and Innate-like Adaptive IFN-γ Immunity against Mycobacteria. Cell. 2020;183:1826-1847.e31 pubmed publisher
	Creyns B, Jacobs I, Verstockt B, Cremer J, Ballet V, Vandecasteele R, et al. Biological Therapy in Inflammatory Bowel Disease Patients Partly Restores Intestinal Innate Lymphoid Cell Subtype Equilibrium. Front Immunol. 2020;11:1847 pubmed publisher
	Ebner F, Morrison E, Bertazzon M, Midha A, Hartmann S, Freund C, et al. CD4+ Th immunogenicity of the Ascaris spp. secreted products. NPJ Vaccines. 2020;5:25 pubmed publisher
	Lamichhane R, Schneider M, de la Harpe S, Harrop T, Hannaway R, Dearden P, et al. TCR- or Cytokine-Activated CD8+ Mucosal-Associated Invariant T Cells Are Rapid Polyfunctional Effectors That Can Coordinate Immune Responses. Cell Rep. 2019;28:3061-3076.e5 pubmed publisher
	Cooper G, Ostridge K, Khakoo S, Wilkinson T, Staples K. Human CD49a+ Lung Natural Killer Cell Cytotoxicity in Response to Influenza A Virus. Front Immunol. 2018;9:1671 pubmed publisher
	Nowak A, Lock D, Bacher P, Hohnstein T, Vogt K, Gottfreund J, et al. CD137+CD154- Expression As a Regulatory T Cell (Treg)-Specific Activation Signature for Identification and Sorting of Stable Human Tregs from In Vitro Expansion Cultures. Front Immunol. 2018;9:199 pubmed publisher
	Smith S, Smits K, Joosten S, van Meijgaarden K, Satti I, Fletcher H, et al. Intracellular Cytokine Staining and Flow Cytometry: Considerations for Application in Clinical Trials of Novel Tuberculosis Vaccines. PLoS ONE. 2015;10:e0138042 pubmed publisher
	Bacher P, Kniemeyer O, Teutschbein J, ThÃ¶n M, VÃ¶disch M, Wartenberg D, et al. Identification of immunogenic antigens from Aspergillus fumigatus by direct multiparameter characterization of specific conventional and regulatory CD4+ T cells. J Immunol. 2014;193:3332-43 pubmed publisher
	Bacher P, Schink C, Teutschbein J, Kniemeyer O, Assenmacher M, Brakhage A, et al. Antigen-reactive T cell enrichment for direct, high-resolution analysis of the human naive and memory Th cell repertoire. J Immunol. 2013;190:3967-76 pubmed publisher
	Iwamoto S, Iwai S, Tsujiyama K, Kurahashi C, Takeshita K, Naoe M, et al. TNF-alpha drives human CD14+ monocytes to differentiate into CD70+ dendritic cells evoking Th1 and Th17 responses. J Immunol. 2007;179:1449-57 pubmed

product information

Antigen :

IFN-gamma

Apps. Abbrev. :

ICFC

Cat # :

502526

Clone :

4S.B3

Item :

PerCP/Cyanine5.5 anti-human IFN-γ

Isotype :

Mouse IgG1, κ

Other Names :

Interferon-γ, Immune interferon, Type II interferon, T cell interferon, Macrophage-activating factor (MAF), IFN-g, IFN-gamma

Size :

100 tests

Price (USD) :

382 USD

Reactivity :

Human

Clonality :

Monoclonal

Host :

Mouse

Conjugate/Tag/Label :

PERCP_CYANINE5.5

Immunogen :

Partially purified, native human IFN-γ

Application Notes :

ELISA or ELISPOT Detection^5: The biotinylated 4S.B3 antibody is useful as a detection antibody for a sandwich ELISA or ELISPOT assay, when used in conjunction with purified NIB42 antibody (Cat. No. 502402/502404) or purified MD-1 antibody (Cat. No. 507502/507513) as the capture antibody.Flow Cytometry^3,4,6-8: The fluorochrome-labeled 4S.B3 antibody is useful for intracellular immunofluorescent staining and flow cytometric analysis to identify IFN-γ -producing cells within mixed cell populations.Additional reported applications (for the relevant formats) include: neutralization^1,2, Western blotting, immunohistochemical staining of paraformaldehyde-fixed, saponin-treated tissue sections, and immunocytochemistry. The 4S.B3 antibody can neutralize the bioactivity of natural or recombinant IFN-γ.Note: For testing human IFN-γ in serum or plasma, BioLegend's ELISA Max™ Sets (Cat. No. 430101 to 430106) are specially developed and recommended.;

more info or order :

BioLegend product webpage