Pacific Blue™ anti-human IFN-γ | BioLegend 502521 product information

product summary

company name :

BioLegend

other brands :

Babco, Signet, Sternberger Monoclonals, Senetek, Covance

product type :

antibody

product name :

Pacific Blue™ anti-human IFN-γ

catalog :

502521

quantity :

25 μg

price :

124 USD

clonality :

monoclonal

host :

mouse

conjugate :

Pacific Blue

clone name :

4S.B3

reactivity :

human

application :

immunocytochemistry, flow cytometry

more info or order :

BioLegend product webpage

citations: 14

Published Application/Species/Sample/Dilution	Reference
flow cytometry; human	Wiernik A, Foley B, Zhang B, Verneris M, Warlick E, Gleason M, et al. Targeting natural killer cells to acute myeloid leukemia in vitro with a CD16 x 33 bispecific killer cell engager and ADAM17 inhibition. Clin Cancer Res. 2013;19:3844-55 pubmed publisher
	Ackerley C, Smith S, Murray P, Amancha P, Arthur R, Zhu Z, et al. The rectal mucosal immune environment and HIV susceptibility among young men who have sex with men. Front Immunol. 2022;13:972170 pubmed publisher
	Qian M, Ling W, Ruan Z. Long non-coding RNA SNHG12 promotes immune escape of ovarian cancer cells through their crosstalk with M2 macrophages. Aging (Albany NY). 2020;12:17122-17136 pubmed publisher
	Zhu H, Blum R, Bernareggi D, Ask E, Wu Z, Hoel H, et al. Metabolic Reprograming via Deletion of CISH in Human iPSC-Derived NK Cells Promotes In Vivo Persistence and Enhances Anti-tumor Activity. Cell Stem Cell. 2020;27:224-237.e6 pubmed publisher
	Ridley M, Fleskens V, Roberts C, Lalnunhlimi S, Alnesf A, O Byrne A, et al. IKZF3/Aiolos Is Associated with but Not Sufficient for the Expression of IL-10 by CD4+ T Cells. J Immunol. 2020;204:2940-2948 pubmed publisher
	Kobayashi Y, Yamada D, Kawai T, Sato Y, Teshima T, Yamada Y, et al. Different immunological effects of the molecular targeted agents sunitinib, everolimus and temsirolimus in patients with renal cell carcinoma. Int J Oncol. 2020;56:999-1013 pubmed publisher
	Li Y, Hermanson D, Moriarity B, Kaufman D. Human iPSC-Derived Natural Killer Cells Engineered with Chimeric Antigen Receptors Enhance Anti-tumor Activity. Cell Stem Cell. 2018;23:181-192.e5 pubmed publisher
	Whitfield S, Taylor C, Risdall J, Griffiths G, Jones J, Williamson E, et al. Interference of the T Cell and Antigen-Presenting Cell Costimulatory Pathway Using CTLA4-Ig (Abatacept) Prevents Staphylococcal Enterotoxin B Pathology. J Immunol. 2017;198:3989-3998 pubmed publisher
	Gleason M, Ross J, Warlick E, Lund T, Verneris M, Wiernik A, et al. CD16xCD33 bispecific killer cell engager (BiKE) activates NK cells against primary MDS and MDSC CD33+ targets. Blood. 2014;123:3016-26 pubmed publisher
	Longhi M, Moss A, Bai A, Wu Y, Huang H, Cheifetz A, et al. Characterization of human CD39+ Th17 cells with suppressor activity and modulation in inflammatory bowel disease. PLoS ONE. 2014;9:e87956 pubmed publisher
	Hong H, Ahmad F, Eberhard J, Bhatnagar N, Bollmann B, Keudel P, et al. Loss of CCR7 expression on CD56(bright) NK cells is associated with a CD56(dim)CD16? NK cell-like phenotype and correlates with HIV viral load. PLoS ONE. 2012;7:e44820 pubmed publisher
	Haque A, Best S, Unosson K, Amante F, de Labastida F, Anstey N, et al. Granzyme B expression by CD8+ T cells is required for the development of experimental cerebral malaria. J Immunol. 2011;186:6148-56 pubmed publisher
	Strowig T, Chijioke O, Carrega P, Arrey F, Meixlsperger S, Rämer P, et al. Human NK cells of mice with reconstituted human immune system components require preactivation to acquire functional competence. Blood. 2010;116:4158-67 pubmed publisher
	Iwamoto S, Iwai S, Tsujiyama K, Kurahashi C, Takeshita K, Naoe M, et al. TNF-alpha drives human CD14+ monocytes to differentiate into CD70+ dendritic cells evoking Th1 and Th17 responses. J Immunol. 2007;179:1449-57 pubmed

product information

Antigen :

IFN-gamma

Apps. Abbrev. :

ICFC

Cat # :

502521

Clone :

4S.B3

Item :

Pacific Blue™ anti-human IFN-γ

Isotype :

Mouse IgG1, κ

Other Names :

Interferon-γ, Immune interferon, Type II interferon, T cell interferon, Macrophage-activating factor (MAF), IFN-g, IFN-gamma

Size :

25 μg

Price (USD) :

124 USD

Reactivity :

Human

Clonality :

Monoclonal

Host :

Mouse

Conjugate/Tag/Label :

Immunogen :

Partially purified, native human IFN-γ

Application Notes :

ELISA or ELISPOT Detection^5: The biotinylated 4S.B3 antibody is useful as a detection antibody for a sandwich ELISA or ELISPOT assay, when used in conjunction with purified NIB42 antibody (Cat. No. 502402/502404) or purified MD-1 antibody (Cat. No. 507502/507513) as the capture antibody.Flow Cytometry^3,4,6-8: The fluorochrome-labeled 4S.B3 antibody is useful for intracellular immunofluorescent staining and flow cytometric analysis to identify IFN-γ -producing cells within mixed cell populations.Additional reported applications (for the relevant formats) include: neutralization^1,2, Western blotting, immunohistochemical staining of paraformaldehyde-fixed, saponin-treated tissue sections, and immunocytochemistry. The 4S.B3 antibody can neutralize the bioactivity of natural or recombinant IFN-γ.Note: For testing human IFN-γ in serum or plasma, BioLegend's ELISA Max™ Sets (Cat. No. 430101 to 430106) are specially developed and recommended.;

more info or order :

BioLegend product webpage