product summary
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company name :
BioLegend
other brands :
Babco, Signet, Sternberger Monoclonals, Senetek, Covance
product type :
antibody
product name :
APC anti-human IFN-γ
catalog :
502512
quantity :
100 tests
price :
284 USD
clonality :
monoclonal
host :
mouse
conjugate :
APC
clone name :
4S.B3
reactivity :
human
application :
immunocytochemistry, flow cytometry
more info or order :
citations: 55
Published Application/Species/Sample/DilutionReference
  • flow cytometry; human; loading ...; fig 6d
Du Y, Peng Q, Cheng D, Pan T, Sun W, Wang H, et al. Cancer cell-expressed BTNL2 facilitates tumour immune escape via engagement with IL-17A-producing γδ T cells. Nat Commun. 2022;13:231 pubmed publisher
  • flow cytometry; human; fig s10
Kono M, Komatsuda H, Yamaki H, Kumai T, Hayashi R, Wakisaka R, et al. Immunomodulation via FGFR inhibition augments FGFR1 targeting T-cell based antitumor immunotherapy for head and neck squamous cell carcinoma. Oncoimmunology. 2022;11:2021619 pubmed publisher
  • flow cytometry; human; fig s11
Wang Y, Mohseni M, Grauel A, Diez J, Guan W, Liang S, et al. SHP2 blockade enhances anti-tumor immunity via tumor cell intrinsic and extrinsic mechanisms. Sci Rep. 2021;11:1399 pubmed publisher
  • flow cytometry; human; fig 2c
Martin E, Minet N, Boschat A, Sanquer S, Sobrino S, Lenoir C, et al. Impaired lymphocyte function and differentiation in CTPS1-deficient patients result from a hypomorphic homozygous mutation. JCI Insight. 2020;5: pubmed publisher
  • flow cytometry; human; loading ...; tbl s9
Mordmuller B, Surat G, Lagler H, Chakravarty S, Ishizuka A, Lalremruata A, et al. Sterile protection against human malaria by chemoattenuated PfSPZ vaccine. Nature. 2017;542:445-449 pubmed publisher
Wu J, Wang X, Zhang M, Mathews P, Kang Y. RXR Agonists Enhance Lenalidomide Anti-Myeloma Activity and T Cell Functions while Retaining Glucose-Lowering Effect. Cells. 2023;12: pubmed publisher
Tan X, Qi C, Zhao X, Sun L, Wu M, Sun W, et al. ERK Inhibition Promotes Engraftment of Allografts by Reprogramming T-Cell Metabolism. Adv Sci (Weinh). 2023;10:e2206768 pubmed publisher
Cheng H, Qiu Y, Xu Y, Chen L, Ma K, Tao M, et al. Extracellular acidosis restricts one-carbon metabolism and preserves T cell stemness. Nat Metab. 2023;5:314-330 pubmed publisher
Peng W, Ye L, Xue Q, Wei X, Wang Z, Xiang X, et al. Silencing of circCRIM1 Drives IGF2BP1-Mediated NSCLC Immune Evasion. Cells. 2023;12: pubmed publisher
Ogura H, Gohda J, Lu X, Yamamoto M, Takesue Y, Son A, et al. Dysfunctional Sars-CoV-2-M protein-specific cytotoxic T lymphocytes in patients recovering from severe COVID-19. Nat Commun. 2022;13:7063 pubmed publisher
Cook C, Taylor M, Liu Y, Schmidt R, Sedgewick A, Kim E, et al. A single-cell transcriptional gradient in human cutaneous memory T cells restricts Th17/Tc17 identity. Cell Rep Med. 2022;3:100715 pubmed publisher
Su W, Zhang J, Yang S, Tang M, Shen Y, Liu C, et al. Galectin-9 contributes to the pathogenesis of atopic dermatitis via T cell immunoglobulin mucin-3. Front Immunol. 2022;13:952338 pubmed publisher
McMurray J, von Borstel A, Taher T, Syrimi E, Taylor G, Sharif M, et al. Transcriptional profiling of human Vδ1 T cells reveals a pathogen-driven adaptive differentiation program. Cell Rep. 2022;39:110858 pubmed publisher
Zhou Z, Yan X, Shi W, Tan K, Shao C, Wang Y, et al. Evaluation of the tumoricidal efficacy of adoptive cell transfer using hepatocellular carcinoma-derived organoids. J Gastrointest Oncol. 2022;13:732-743 pubmed publisher
Chandran A, Rosenheim J, Nageswaran G, Swadling L, Pollara G, Gupta R, et al. Rapid synchronous type 1 IFN and virus-specific T cell responses characterize first wave non-severe SARS-CoV-2 infections. Cell Rep Med. 2022;3:100557 pubmed publisher
Wang H, Cao K, Liu S, Xu Y, Tang L. Tim-3 Expression Causes NK Cell Dysfunction in Type 2 Diabetes Patients. Front Immunol. 2022;13:852436 pubmed publisher
Veatch J, Lee S, Shasha C, Singhi N, Szeto J, Moshiri A, et al. Neoantigen-specific CD4+ T cells in human melanoma have diverse differentiation states and correlate with CD8+ T cell, macrophage, and B cell function. Cancer Cell. 2022;40:393-409.e9 pubmed publisher
Zeng Q, Song J, Wang D, Sun X, Xiao Y, Zhang H, et al. Identification of Sorafenib as a Treatment for Type 1 Diabetes. Front Immunol. 2022;13:740805 pubmed publisher
Liu W. Evaluating Human Natural Killer Cells Antibody-dependent Cellular Cytotoxicity (ADCC) Using Plate-bound Anti-CD16 Antibodies. Bio Protoc. 2022;12:e4285 pubmed publisher
Anderson A, Wu I, Parrillo A, Wolf M, Maestas D, Graham I, et al. An immunologically active, adipose-derived extracellular matrix biomaterial for soft tissue reconstruction: concept to clinical trial. NPJ Regen Med. 2022;7:6 pubmed publisher
Swadling L, Diniz M, Schmidt N, Amin O, Chandran A, Shaw E, et al. Pre-existing polymerase-specific T cells expand in abortive seronegative SARS-CoV-2. Nature. 2022;601:110-117 pubmed publisher
Shen C, Zhang Z, Tian Y, Li F, Zhou L, Jiang W, et al. Sulforaphane enhances the antitumor response of chimeric antigen receptor T cells by regulating PD-1/PD-L1 pathway. BMC Med. 2021;19:283 pubmed publisher
Li M, Garforth S, O Connor K, Su H, Lee D, Celikgil A, et al. T cell receptor-targeted immunotherapeutics drive selective in vivo HIV- and CMV-specific T cell expansion in humanized mice. J Clin Invest. 2021;131: pubmed publisher
Devalraju K, Neela V, Krovvidi S, Vankayalapati R, Valluri V. Defective expansion and function of memory like natural killer cells in HIV+ individuals with latent tuberculosis infection. PLoS ONE. 2021;16:e0257185 pubmed publisher
Wang Y, Wang F, Wang L, Qiu S, Yao Y, Yan C, et al. NAD+ supplement potentiates tumor-killing function by rescuing defective TUB-mediated NAMPT transcription in tumor-infiltrated T cells. Cell Rep. 2021;36:109516 pubmed publisher
Guo S, Huang C, Han F, Chen B, Ding Y, Zhao Y, et al. Gastric Cancer Mesenchymal Stem Cells Inhibit NK Cell Function through mTOR Signalling to Promote Tumour Growth. Stem Cells Int. 2021;2021:9989790 pubmed publisher
Shao L, He Q, Wang J, He F, Lin S, Wu L, et al. MicroRNA-326 attenuates immune escape and prevents metastasis in lung adenocarcinoma by targeting PD-L1 and B7-H3. Cell Death Discov. 2021;7:145 pubmed publisher
Cai J, Gao H, Zhao J, Hu S, Liang X, Yang Y, et al. Infection with a newly designed dual fluorescent reporter HIV-1 effectively identifies latently infected CD4+ T cells. elife. 2021;10: pubmed publisher
Prakash M, Stojanovska L, Feehan J, Nurgali K, Donald E, Plebanski M, et al. Anti-cancer effects of polyphenol-rich sugarcane extract. PLoS ONE. 2021;16:e0247492 pubmed publisher
O Donoghue G, Bugaj L, Anderson W, Daniels K, Rawlings D, Lim W. T cells selectively filter oscillatory signals on the minutes timescale. Proc Natl Acad Sci U S A. 2021;118: pubmed publisher
Baban B, Braun M, Khodadadi H, Ward A, Alverson K, Malik A, et al. AMPK induces regulatory innate lymphoid cells after traumatic brain injury. JCI Insight. 2021;6: pubmed publisher
Tesch S, Abdirama D, Grießbach A, Brand H, Goerlich N, Humrich J, et al. Identification and characterization of antigen-specific CD4+ T cells targeting renally expressed antigens in human lupus nephritis with two independent methods. Sci Rep. 2020;10:21312 pubmed publisher
Harshe R, Xie A, Vuerich M, Frank L, Gromova B, Zhang H, et al. Endogenous antisense RNA curbs CD39 expression in Crohn's disease. Nat Commun. 2020;11:5894 pubmed publisher
Fang Y, Wang L, Wan C, Sun Y, Van der Jeught K, Zhou Z, et al. MAL2 drives immune evasion in breast cancer by suppressing tumor antigen presentation. J Clin Invest. 2021;131: pubmed publisher
Faust H, Zhang H, Han J, Wolf M, Jeon O, Sadtler K, et al. IL-17 and immunologically induced senescence regulate response to injury in osteoarthritis. J Clin Invest. 2020;130:5493-5507 pubmed publisher
Evans R, Antonelou M, Sathiananthamoorthy S, Rega M, Henderson S, Ceron Gutierrez L, et al. Inherited salt-losing tubulopathies are associated with immunodeficiency due to impaired IL-17 responses. Nat Commun. 2020;11:4368 pubmed publisher
Pandey A, Stawiski E, Durinck S, Gowda H, Goldstein L, Barbhuiya M, et al. Integrated genomic analysis reveals mutated ELF3 as a potential gallbladder cancer vaccine candidate. Nat Commun. 2020;11:4225 pubmed publisher
Port J, Wozniak D, Oestereich L, Pallasch E, Becker Ziaja B, M xfc ller J, et al. Severe Human Lassa Fever Is Characterized by Nonspecific T-Cell Activation and Lymphocyte Homing to Inflamed Tissues. J Virol. 2020;94: pubmed publisher
Festag J, Thelemann T, Schell M, Raith S, Michel S, Jaschinski F, et al. Preventing ATP Degradation by ASO-Mediated Knockdown of CD39 and CD73 Results in A2aR-Independent Rescue of T Cell Proliferation. Mol Ther Nucleic Acids. 2020;21:656-669 pubmed publisher
Ni J, Wang X, Stojanovic A, Zhang Q, Wincher M, Bühler L, et al. Single-Cell RNA Sequencing of Tumor-Infiltrating NK Cells Reveals that Inhibition of Transcription Factor HIF-1α Unleashes NK Cell Activity. Immunity. 2020;52:1075-1087.e8 pubmed publisher
Kim J, Ghosh A, Ayithan N, Romani S, Khanam A, Park J, et al. Circulating serum HBsAg level is a biomarker for HBV-specific T and B cell responses in chronic hepatitis B patients. Sci Rep. 2020;10:1835 pubmed publisher
Ge C, Monk I, Pizzolla A, Wang N, Bedford J, Stinear T, et al. Bystander Activation of Pulmonary Trm Cells Attenuates the Severity of Bacterial Pneumonia by Enhancing Neutrophil Recruitment. Cell Rep. 2019;29:4236-4244.e3 pubmed publisher
Yang C, Siebert J, Burns R, Gerbec Z, Bonacci B, Rymaszewski A, et al. Heterogeneity of human bone marrow and blood natural killer cells defined by single-cell transcriptome. Nat Commun. 2019;10:3931 pubmed publisher
Wei J, Luo C, Wang Y, Guo Y, Dai H, Tong C, et al. PD-1 silencing impairs the anti-tumor function of chimeric antigen receptor modified T cells by inhibiting proliferation activity. J Immunother Cancer. 2019;7:209 pubmed publisher
Draganov D, Santidrian A, Minev I, Nguyen D, Kilinç M, Petrov I, et al. Delivery of oncolytic vaccinia virus by matched allogeneic stem cells overcomes critical innate and adaptive immune barriers. J Transl Med. 2019;17:100 pubmed publisher
Waight J, Chand D, Dietrich S, Gombos R, Horn T, Gonzalez A, et al. Selective FcγR Co-engagement on APCs Modulates the Activity of Therapeutic Antibodies Targeting T Cell Antigens. Cancer Cell. 2018;33:1033-1047.e5 pubmed publisher
Boutboul D, Kuehn H, Van de Wyngaert Z, Niemela J, Callebaut I, Stoddard J, et al. Dominant-negative IKZF1 mutations cause a T, B, and myeloid cell combined immunodeficiency. J Clin Invest. 2018;128:3071-3087 pubmed publisher
Du Q, Wu X, Wang T, Yang X, Wang Z, Niu Y, et al. Porcine Circovirus Type 2 Suppresses IL-12p40 Induction via Capsid/gC1qR-Mediated MicroRNAs and Signalings. J Immunol. 2018;201:533-547 pubmed publisher
Pahl J, Koch J, Götz J, Arnold A, Reusch U, Gantke T, et al. CD16A Activation of NK Cells Promotes NK Cell Proliferation and Memory-Like Cytotoxicity against Cancer Cells. Cancer Immunol Res. 2018;6:517-527 pubmed publisher
Zhang X, Lian X, Dai Z, Zheng H, Chen X, Zheng Y. ?3-Deletion Isoform of HLA-A11 Modulates Cytotoxicity of NK Cells: Correlations with HIV-1 Infection of Cells. J Immunol. 2017;199:2030-2042 pubmed publisher
Roybal K, Williams J, Morsut L, Rupp L, Kolinko I, Choe J, et al. Engineering T Cells with Customized Therapeutic Response Programs Using Synthetic Notch Receptors. Cell. 2016;167:419-432.e16 pubmed publisher
Tian X, Zhang A, Qiu C, Wang W, Yang Y, Qiu C, et al. The upregulation of LAG-3 on T cells defines a subpopulation with functional exhaustion and correlates with disease progression in HIV-infected subjects. J Immunol. 2015;194:3873-82 pubmed publisher
Warfield K, Goetzmann J, Biggins J, Kasda M, Unfer R, Vu H, et al. Vaccinating captive chimpanzees to save wild chimpanzees. Proc Natl Acad Sci U S A. 2014;111:8873-6 pubmed publisher
Longhi M, Moss A, Bai A, Wu Y, Huang H, Cheifetz A, et al. Characterization of human CD39+ Th17 cells with suppressor activity and modulation in inflammatory bowel disease. PLoS ONE. 2014;9:e87956 pubmed publisher
Norian L, Rodriguez P, O Mara L, Zabaleta J, Ochoa A, Cella M, et al. Tumor-infiltrating regulatory dendritic cells inhibit CD8+ T cell function via L-arginine metabolism. Cancer Res. 2009;69:3086-94 pubmed publisher
product information
Antigen :
IFN-gamma
Apps. Abbrev. :
ICFC
Cat # :
502512
Clone :
4S.B3
Item :
APC anti-human IFN-γ
Isotype :
Mouse IgG1, κ
Other Names :
Interferon-γ, Immune interferon, Type II interferon, T cell interferon, Macrophage-activating factor (MAF), IFN-g, IFN-gamma
Size :
100 tests
Price (USD) :
284 USD
Reactivity :
Human
Clonality :
Monoclonal
Host :
Mouse
Conjugate/Tag/Label :
APC
Immunogen :
Partially purified, native human IFN-γ
Application Notes :
ELISA or ELISPOT Detection^5: The biotinylated 4S.B3 antibody is useful as a detection antibody for a sandwich ELISA or ELISPOT assay, when used in conjunction with purified NIB42 antibody (Cat. No. 502402/502404) or purified MD-1 antibody (Cat. No. 507502/507513) as the capture antibody.Flow Cytometry^3,4,6-8: The fluorochrome-labeled 4S.B3 antibody is useful for intracellular immunofluorescent staining and flow cytometric analysis to identify IFN-γ -producing cells within mixed cell populations.Additional reported applications (for the relevant formats) include: neutralization^1,2, Western blotting, immunohistochemical staining of paraformaldehyde-fixed, saponin-treated tissue sections, and immunocytochemistry. The 4S.B3 antibody can neutralize the bioactivity of natural or recombinant IFN-γ.Note: For testing human IFN-γ in serum or plasma, BioLegend's ELISA Max™ Sets (Cat. No. 430101 to 430106) are specially developed and recommended.;
more info or order :
company information
BioLegend
8999 BioLegend Way
San Diego, CA 92121
customerserv@biolegend.com
https://www.biolegend.com
headquarters: USA