product summary
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company name :
BioLegend
other brands :
Babco, Signet, Sternberger Monoclonals, Senetek, Covance
product type :
antibody
product name :
FITC anti-human IFN-γ
catalog :
502506
quantity :
100 tests
price :
223 USD
clonality :
monoclonal
host :
mouse
conjugate :
FITC
clone name :
4S.B3
reactivity :
human
application :
immunocytochemistry, flow cytometry
more info or order :
citations: 39
Published Application/Species/Sample/DilutionReference
  • flow cytometry; human; loading ...; fig 4a
Zhu H, Hu F, Sun X, Zhang X, Zhu L, Liu X, et al. CD16+ Monocyte Subset Was Enriched and Functionally Exacerbated in Driving T-Cell Activation and B-Cell Response in Systemic Lupus Erythematosus. Front Immunol. 2016;7:512 pubmed
Zhang H, Jadhav R, Cao W, Goronzy I, Zhao T, Jin J, et al. Aging-associated HELIOS deficiency in naive CD4+ T cells alters chromatin remodeling and promotes effector cell responses. Nat Immunol. 2023;24:96-109 pubmed publisher
Du Q, Yang X, Ma M, Zhang Q, Zhu C, Shi T, et al. The gC1qR Binding Site Mutant PCV2 Is a Potential Vaccine Strain That Does Not Impair Memory CD4+ T-Cell Generation by Vaccines. J Virol. 2022;96:e0095922 pubmed publisher
Pan Y, Su L. Identification of Human Antigen-Specific T Cells Using Class II MHC Tetramer Staining and Enrichment. Methods Mol Biol. 2022;2574:31-40 pubmed publisher
Olafsdottir T, Bjarnadóttir K, Norddahl G, Halldorsson G, Melsted P, Gunnarsdottir K, et al. HLA alleles, disease severity, and age associate with T-cell responses following infection with SARS-CoV-2. Commun Biol. 2022;5:914 pubmed publisher
Duan L, Cui X, Zhu K, Yao L, Wang G, Cao W, et al. SARS-CoV-2 vaccine-induced antibody and T cell response in SARS-CoV-1 survivors. Cell Rep. 2022;40:111284 pubmed publisher
Lai Y, Zhao S, Chen B, Huang Y, Guo C, Li M, et al. Iron controls T helper cell pathogenicity by promoting glucose metabolism in autoimmune myopathy. Clin Transl Med. 2022;12:e999 pubmed publisher
Hirschberger S, Gellert L, Effinger D, Muenchhoff M, Herrmann M, Briegel J, et al. Ketone Bodies Improve Human CD8+ Cytotoxic T-Cell Immune Response During COVID-19 Infection. Front Med (Lausanne). 2022;9:923502 pubmed publisher
Carre C, Wong G, Narang V, Tan C, Chong J, Chin H, et al. Endoplasmic reticulum stress response and bile acid signatures associate with multi-strain seroresponsiveness during elderly influenza vaccination. iScience. 2021;24:102970 pubmed publisher
Cohen C, Li A, Hachim A, Hui D, Kwan M, Tsang O, et al. SARS-CoV-2 specific T cell responses are lower in children and increase with age and time after infection. Nat Commun. 2021;12:4678 pubmed publisher
Hirschberger S, Strauß G, Effinger D, Marstaller X, Ferstl A, Müller M, et al. Very-low-carbohydrate diet enhances human T-cell immunity through immunometabolic reprogramming. EMBO Mol Med. 2021;13:e14323 pubmed publisher
Hamilton J, Tsuchida C, Nguyen D, Shy B, McGarrigle E, Sandoval Espinoza C, et al. Targeted delivery of CRISPR-Cas9 and transgenes enables complex immune cell engineering. Cell Rep. 2021;35:109207 pubmed publisher
Pan Y, Aiamkitsumrit B, Bartolo L, Wang Y, Lavery C, Marc A, et al. Vaccination reshapes the virus-specific T cell repertoire in unexposed adults. Immunity. 2021;54:1245-1256.e5 pubmed publisher
Li X, Dong L, Liu J, Wang C, Zhang Y, Mei Q, et al. Low-Dose Decitabine Augments the Activation and Anti-Tumor Immune Response of IFN-γ+ CD4+ T Cells Through Enhancing IκBα Degradation and NF-κB Activation. Front Cell Dev Biol. 2021;9:647713 pubmed publisher
Priyanto H, Chua E, Hutchinson P, Nugraha J, Amin M. A decrease in PPD specific CD4 T cell CD38 and HLA-DR expression in pulmonary tuberculosis patients after 8 weeks of therapy correlates with successful anti-tuberculosis treatment. J Clin Tuberc Other Mycobact Dis. 2021;22:100214 pubmed publisher
Zou F, Tan J, Liu T, Liu B, Tang Y, Zhang H, et al. The CD39+ HBV surface protein-targeted CAR-T and personalized tumor-reactive CD8+ T cells exhibit potent anti-HCC activity. Mol Ther. 2021;29:1794-1807 pubmed publisher
Corrado M, Edwards Hicks J, Villa M, Flachsmann L, Sanin D, Jacobs M, et al. Dynamic Cardiolipin Synthesis Is Required for CD8+ T Cell Immunity. Cell Metab. 2020;32:981-995.e7 pubmed publisher
Pierce C, Preston Hurlburt P, Dai Y, Aschner C, Cheshenko N, Galen B, et al. Immune responses to SARS-CoV-2 infection in hospitalized pediatric and adult patients. Sci Transl Med. 2020;12: pubmed publisher
Hess N, Hudson A, Hematti P, Gumperz J. Early T Cell Activation Metrics Predict Graft-versus-Host Disease in a Humanized Mouse Model of Hematopoietic Stem Cell Transplantation. J Immunol. 2020;205:272-281 pubmed publisher
Zhou Y, Shao N, Bessa de Castro R, Zhang P, Ma Y, Liu X, et al. Evaluation of Single-Cell Cytokine Secretion and Cell-Cell Interactions with a Hierarchical Loading Microwell Chip. Cell Rep. 2020;31:107574 pubmed publisher
Lo Presti E, Mocciaro F, Mitri R, Corsale A, Di Simone M, Vieni S, et al. Analysis of colon-infiltrating γδ T cells in chronic inflammatory bowel disease and in colitis-associated cancer. J Leukoc Biol. 2020;108:749-760 pubmed publisher
Preglej T, Hamminger P, Luu M, Bulat T, Andersen L, Göschl L, et al. Histone deacetylases 1 and 2 restrain CD4+ cytotoxic T lymphocyte differentiation. JCI Insight. 2020;5: pubmed publisher
Lu H, Jin L, Huang H, Ha S, Yang H, Chang R, et al. Trophoblast-derived CXCL12 promotes CD56bright CD82- CD29+ NK cell enrichment in the decidua. Am J Reprod Immunol. 2020;83: pubmed publisher
Shokri M, Bozorgmehr M, Ghanavatinejad A, Falak R, Aleahmad M, Kazemnejad S, et al. Human menstrual blood-derived stromal/stem cells modulate functional features of natural killer cells. Sci Rep. 2019;9:10007 pubmed publisher
Zuazo M, Arasanz H, Fernández Hinojal G, García Granda M, Gato M, Bocanegra A, et al. Functional systemic CD4 immunity is required for clinical responses to PD-L1/PD-1 blockade therapy. EMBO Mol Med. 2019;: pubmed publisher
Stein R, Ebert S, Schlahsa L, Scholz C, Braun M, Hauck P, et al. Cognate Nonlytic Interactions between CD8+ T Cells and Breast Cancer Cells Induce Cancer Stem Cell-like Properties. Cancer Res. 2019;79:1507-1519 pubmed publisher
Yu J, Zhang H, Sun S, Sun S, Li L. The effects of Tim-3 activation on T-cells in gastric cancer progression. Oncol Lett. 2019;17:1461-1466 pubmed publisher
Kuranda K, Jean Alphonse P, Leborgne C, Hardet R, Collaud F, Marmier S, et al. Exposure to wild-type AAV drives distinct capsid immunity profiles in humans. J Clin Invest. 2018;128:5267-5279 pubmed publisher
Morimoto Y, Hirahara K, Kiuchi M, Wada T, Ichikawa T, Kanno T, et al. Amphiregulin-Producing Pathogenic Memory T Helper 2 Cells Instruct Eosinophils to Secrete Osteopontin and Facilitate Airway Fibrosis. Immunity. 2018;49:134-150.e6 pubmed publisher
Wang F, Li B, Wei Y, Zhao Y, Wang L, Zhang P, et al. Tumor-derived exosomes induce PD1+ macrophage population in human gastric cancer that promotes disease progression. Oncogenesis. 2018;7:41 pubmed publisher
Meraviglia S, Lo Presti E, Tosolini M, La Mendola C, Orlando V, Todaro M, et al. Distinctive features of tumor-infiltrating ?? T lymphocytes in human colorectal cancer. Oncoimmunology. 2017;6:e1347742 pubmed publisher
Fisher J, Abramowski P, Wisidagamage Don N, Flutter B, Capsomidis A, Cheung G, et al. Avoidance of On-Target Off-Tumor Activation Using a Co-stimulation-Only Chimeric Antigen Receptor. Mol Ther. 2017;25:1234-1247 pubmed publisher
Kessler B, Rinchai D, Kewcharoenwong C, Nithichanon A, Biggart R, Hawrylowicz C, et al. Interleukin 10 inhibits pro-inflammatory cytokine responses and killing of Burkholderia pseudomallei. Sci Rep. 2017;7:42791 pubmed publisher
Vacaflores A, Freedman S, Chapman N, Houtman J. Pretreatment of activated human CD8 T cells with IL-12 leads to enhanced TCR-induced signaling and cytokine production. Mol Immunol. 2017;81:1-15 pubmed publisher
Vacaflores A, Chapman N, Harty J, Richer M, Houtman J. Exposure of Human CD4 T Cells to IL-12 Results in Enhanced TCR-Induced Cytokine Production, Altered TCR Signaling, and Increased Oxidative Metabolism. PLoS ONE. 2016;11:e0157175 pubmed publisher
Lu T, Yu S, Liu Y, Yin C, Ye J, Liu Z, et al. Aberrant Circulating Th17 Cells in Patients with B-Cell Non-Hodgkin's Lymphoma. PLoS ONE. 2016;11:e0148044 pubmed publisher
Rochman Y, Yukawa M, Kartashov A, Barski A. Functional characterization of human T cell hyporesponsiveness induced by CTLA4-Ig. PLoS ONE. 2015;10:e0122198 pubmed publisher
Spencer A, Cottingham M, Jenks J, Longley R, Capone S, Colloca S, et al. Enhanced vaccine-induced CD8+ T cell responses to malaria antigen ME-TRAP by fusion to MHC class ii invariant chain. PLoS ONE. 2014;9:e100538 pubmed publisher
Blom L, Poulsen L. IL-1 family members IL-18 and IL-33 upregulate the inflammatory potential of differentiated human Th1 and Th2 cultures. J Immunol. 2012;189:4331-7 pubmed publisher
product information
Antigen :
IFN-gamma
Apps. Abbrev. :
ICFC
Cat # :
502506
Clone :
4S.B3
Item :
FITC anti-human IFN-γ
Isotype :
Mouse IgG1, κ
Other Names :
Interferon-γ, Immune interferon, Type II interferon, T cell interferon, Macrophage-activating factor (MAF), IFN-g, IFN-gamma
Size :
100 tests
Price (USD) :
223 USD
Reactivity :
Human
Clonality :
Monoclonal
Host :
Mouse
Conjugate/Tag/Label :
FITC
Immunogen :
Partially purified, native human IFN-γ
Application Notes :
ELISA or ELISPOT Detection^5: The biotinylated 4S.B3 antibody is useful as a detection antibody for a sandwich ELISA or ELISPOT assay, when used in conjunction with purified NIB42 antibody (Cat. No. 502402/502404) or purified MD-1 antibody (Cat. No. 507502/507513) as the capture antibody.Flow Cytometry^3,4,6-8: The fluorochrome-labeled 4S.B3 antibody is useful for intracellular immunofluorescent staining and flow cytometric analysis to identify IFN-γ -producing cells within mixed cell populations.Additional reported applications (for the relevant formats) include: neutralization^1,2, Western blotting, immunohistochemical staining of paraformaldehyde-fixed, saponin-treated tissue sections, and immunocytochemistry. The 4S.B3 antibody can neutralize the bioactivity of natural or recombinant IFN-γ.Note: For testing human IFN-γ in serum or plasma, BioLegend's ELISA Max™ Sets (Cat. No. 430101 to 430106) are specially developed and recommended.;
more info or order :
company information
BioLegend
8999 BioLegend Way
San Diego, CA 92121
customerserv@biolegend.com
https://www.biolegend.com
headquarters: USA