Ultra-LEAF™ Purified anti-human IL-10 | BioLegend 501428 product information

product summary

company name :

BioLegend

other brands :

Babco, Signet, Sternberger Monoclonals, Senetek, Covance

product type :

antibody

product name :

Ultra-LEAF™ Purified anti-human IL-10

catalog :

501428

quantity :

1 mg

price :

644 USD

clonality :

monoclonal

host :

rat

conjugate :

nonconjugated

clone name :

JES3-9D7

reactivity :

human

application :

ELISA, neutralization, flow cytometry, immunohistochemistry - paraffin section

more info or order :

BioLegend product webpage

citations: 9

Published Application/Species/Sample/Dilution	Reference
flow cytometry; human; loading ...	Jeong J, Choi S, Ahn S, Oh J, Kim Y, Lee C, et al. Neutrophil extracellular trap clearance by synovial macrophages in gout. Arthritis Res Ther. 2021;23:88 pubmed publisher
flow cytometry; human; loading ...; fig s6d	Tai Y, Lin L, Xing L, Cho S, Yu T, Acharya C, et al. APRIL signaling via TACI mediates immunosuppression by T regulatory cells in multiple myeloma: therapeutic implications. Leukemia. 2019;33:426-438 pubmed publisher
	Jiang J, Yan S, Zhou X, Zhou J, Bai X, Tan Q, et al. Crosstalk Between Circulating Follicular T Helper Cells and Regulatory B Cells in Children With Extrinsic Atopic Dermatitis. Front Immunol. 2021;12:785549 pubmed publisher
	Fu B, Wang D, Shen X, Guo C, Liu Y, Ye Y, et al. Immunomodulation Induced During Interferon-α Therapy Impairs the Anti-HBV Immune Response Through CD24+CD38hi B Cells. Front Immunol. 2020;11:591269 pubmed publisher
	Gruber C, Patel R, Trachtman R, Lepow L, Amanat F, Krammer F, et al. Mapping Systemic Inflammation and Antibody Responses in Multisystem Inflammatory Syndrome in Children (MIS-C). Cell. 2020;183:982-995.e14 pubmed publisher
	Kalvala A, Wallet P, Yang L, Wang C, Li H, Nam A, et al. Phenotypic Switching of Naïve T Cells to Immune-Suppressive Treg-Like Cells by Mutant KRAS. J Clin Med. 2019;8: pubmed publisher
	Almeida V, Avelar Freitas B, Santos M, Costa L, Silva T, Pereira W, et al. Inhibitory effect of the Pseudobrickellia brasiliensis (Spreng) R.M. King & H. Rob. aqueous extract on human lymphocyte proliferation and IFN-? and TNF-? production in vitro. Braz J Med Biol Res. 2017;50:e5163 pubmed publisher
	Mou W, Han W, Ma X, Wang X, Qin H, Zhao W, et al. γδTFH cells promote B cell maturation and antibody production in neuroblastoma. BMC Immunol. 2017;18:36 pubmed publisher
	Aravena O, Ferrier A, Menon M, Mauri C, Aguillon J, Soto L, et al. TIM-1 defines a human regulatory B cell population that is altered in frequency and function in systemic sclerosis patients. Arthritis Res Ther. 2017;19:8 pubmed publisher

product information

Antigen :

IL-10

Apps. Abbrev. :

ELISA Capture, IHC-P, Neut

Cat # :

501428

Clone :

JES3-9D7

Item :

Ultra-LEAF™ Purified anti-human IL-10

Isotype :

Rat IgG1, κ

Other Names :

Interleukin-10, B cell derived T cell growth factor (B-TCGF), Cytokine synthesis inhibitory factor (CSIF), T-cell growth inhibitory factor (TGIF)

Size :

1 mg

Price (USD) :

644 USD

Reactivity :

Human

Clonality :

Monoclonal

Host :

Rat

Conjugate/Tag/Label :

ULEAF

Immunogen :

COS - expressed, recombinant human IL-10

Application Notes :

ELISA Capture^1-5 or ELISPOT Capture^6: The Ultra-LEAFpurified JES3-9D7 antibody is useful as the capture antibody in a sandwich ELISA, when used in conjunction with the biotinylated JES3-12G8 antibody (Cat. No. 501502) as the detecting antibody and recombinant human IL-10 (Cat. No. 571009) as the standard. The Ultra-LEAF™ purified antibody is suggested for ELISPOT capture.Neutralization^1-3,9: The Ultra-LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for neutralization of human IL-10 bioactivity (Cat. Nos. 501427 & 501428). The JES3-9D7 antibody can neutralize the bioactivity of natural or recombinant IL-10.Additional reported applications (for the relevant formats) include: immunohistochemical staining^12.Note: For testing human IL-10 in serum or plasma, BioLegend's ELISA Max™ Sets (Cat. Nos. 430601 & 430606) are specially developed and recommended.The JES3-9D7 antibody reacts with human and viral interleukin-10 (IL-10).;

more info or order :

BioLegend product webpage