Ultra-LEAF™ Purified anti-human IL-4 | BioLegend 500839 product information

product summary

company name :

BioLegend

other brands :

Babco, Signet, Sternberger Monoclonals, Senetek, Covance

product type :

antibody

product name :

Ultra-LEAF™ Purified anti-human IL-4

catalog :

500839

quantity :

5 mg

price :

539 USD

clonality :

monoclonal

host :

rat

conjugate :

nonconjugated

clone name :

MP4-25D2

reactivity :

human

application :

ELISA, immunohistochemistry, immunocytochemistry, neutralization, mass cytometry

more info or order :

BioLegend product webpage

citations: 11

Published Application/Species/Sample/Dilution	Reference
mass cytometry; human; loading ...; fig s1a	Ingelfinger F, Krishnarajah S, Kramer M, Utz S, Galli E, Lutz M, et al. Single-cell profiling of myasthenia gravis identifies a pathogenic T cell signature. Acta Neuropathol. 2021;141:901-915 pubmed publisher
	Jiang J, Yan S, Zhou X, Zhou J, Bai X, Tan Q, et al. Crosstalk Between Circulating Follicular T Helper Cells and Regulatory B Cells in Children With Extrinsic Atopic Dermatitis. Front Immunol. 2021;12:785549 pubmed publisher
	Shen C, Liu C, Zhang Z, Ping Y, Shao J, Tian Y, et al. PD-1 Affects the Immunosuppressive Function of Group 2 Innate Lymphoid Cells in Human Non-Small Cell Lung Cancer. Front Immunol. 2021;12:680055 pubmed publisher
	Viaud M, Abdel Wahab O, Gall J, Ivanov S, Guinamard R, Sore S, et al. ABCA1 Exerts Tumor-Suppressor Function in Myeloproliferative Neoplasms. Cell Rep. 2020;30:3397-3410.e5 pubmed publisher
	van de Garde M, van Westen E, Poelen M, Rots N, van Els C. Prediction and Validation of Immunogenic Domains of Pneumococcal Proteins Recognized by Human CD4+ T Cells. Infect Immun. 2019;87: pubmed publisher
	Schlenner S, Pasciuto E, Lagou V, Burton O, Prezzemolo T, Junius S, et al. NFIL3 mutations alter immune homeostasis and sensitise for arthritis pathology. Ann Rheum Dis. 2019;78:342-349 pubmed publisher
	Das A, Rouault Pierre K, Kamdar S, Gomez Tourino I, Wood K, Donaldson I, et al. Adaptive from Innate: Human IFN-Î³+CD4+ T Cells Can Arise Directly from CXCL8-Producing Recent Thymic Emigrants in Babies and Adults. J Immunol. 2017;199:1696-1705 pubmed publisher
	Mou W, Han W, Ma X, Wang X, Qin H, Zhao W, et al. γδTFH cells promote B cell maturation and antibody production in neuroblastoma. BMC Immunol. 2017;18:36 pubmed publisher
	Sulzer D, Alcalay R, Garretti F, Cote L, Kanter E, Agin Liebes J, et al. T cells from patients with Parkinson's disease recognize α-synuclein peptides. Nature. 2017;546:656-661 pubmed publisher
	Jiang J, Liu C, Liu M, Shen Y, Hu X, Wang Q, et al. OX40 signaling is involved in the autoactivation of CD4+CD28- T cells and contributes to the pathogenesis of autoimmune arthritis. Arthritis Res Ther. 2017;19:67 pubmed publisher
	Aravena O, Ferrier A, Menon M, Mauri C, Aguillon J, Soto L, et al. TIM-1 defines a human regulatory B cell population that is altered in frequency and function in systemic sclerosis patients. Arthritis Res Ther. 2017;19:8 pubmed publisher

product information

Antigen :

IL-4

Apps. Abbrev. :

ELISA, CyTOF®, Neut, ICFC, IHC

Cat # :

500839

Clone :

MP4-25D2

Item :

Ultra-LEAF™ Purified anti-human IL-4

Isotype :

Rat IgG1, κ

Other Names :

Interleukin-4, IaIF, BSF-1, HCGF, MCGF-2, MFF, TCGF-2

Size :

5 mg

Price (USD) :

539 USD

Reactivity :

Human

Clonality :

Monoclonal

Host :

Rat

Conjugate/Tag/Label :

ULEAF

Immunogen :

CHO-expressed, recombinant human IL-4

Application Notes :

ELISA Detection^1,3 or ELISPOT Detection^4,5: The biotinylated MP4-25D2 antibody is useful as a detection antibody for a sandwich ELISA or ELISPOT assay, when used in conjunction with purified 8D4-8 antibody (Cat. No. 500702/500707) as the capture antibody.Flow Cytometry^6,9: The fluorochrome-labeled MP4-25D2 antibody is useful for intracellular immunofluorescent staining and flow cytometric analysis to identify IL-4 -producing cells within mixed cell populations.Neutralization^1-3: The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for neutralization of human IL-4 bioactivity (Cat. No. 500815). The MP4-25D2 antibody can neutralize the bioactivity of natural or recombinant IL-4.;

more info or order :

BioLegend product webpage