Brilliant Violet 421™ anti-human IL-4 | BioLegend 500826 product information

product summary

company name :

BioLegend

other brands :

Babco, Signet, Sternberger Monoclonals, Senetek, Covance

product type :

antibody

product name :

Brilliant Violet 421™ anti-human IL-4

catalog :

500826

quantity :

100 tests

price :

386 USD

clonality :

monoclonal

host :

rat

conjugate :

BV421

clone name :

MP4-25D2

reactivity :

human

application :

immunocytochemistry, flow cytometry

more info or order :

BioLegend product webpage

citations: 13

Published Application/Species/Sample/Dilution	Reference
flow cytometry; human; 2 ul/test	Bowcutt R, Malter L, Chen L, Wolff M, Robertson I, Rifkin D, et al. Isolation and cytokine analysis of lamina propria lymphocytes from mucosal biopsies of the human colon. J Immunol Methods. 2015;421:27-35 pubmed publisher
	Mashayekhi M, Wanjalla C, Warren C, Simmons J, Ghoshal K, Pilkinton M, et al. The soluble epoxide hydrolase inhibitor GSK2256294 decreases the proportion of adipose pro-inflammatory T cells. Prostaglandins Other Lipid Mediat. 2022;158:106604 pubmed publisher
	Kim S, Tayar J, Fu S, Ke D, Norry E, Sun A, et al. Newly developed pseudogout arthritis after therapy with MAGE-A4 directed TCR T cells responded to treatment with tocilizumab. J Immunother Cancer. 2021;9: pubmed publisher
	Vanderbeke L, Van Mol P, van Herck Y, De Smet F, Humblet Baron S, Martinod K, et al. Monocyte-driven atypical cytokine storm and aberrant neutrophil activation as key mediators of COVID-19 disease severity. Nat Commun. 2021;12:4117 pubmed publisher
	Pušnik J, Richter E, Schulte B, Dolscheid Pommerich R, Bode C, Putensen C, et al. Memory B cells targeting SARS-CoV-2 spike protein and their dependence on CD4+ T cell help. Cell Rep. 2021;35:109320 pubmed publisher
	Camiolo M, Zhou X, Oriss T, Yan Q, Gorry M, Horne W, et al. High-dimensional profiling clusters asthma severity by lymphoid and non-lymphoid status. Cell Rep. 2021;35:108974 pubmed publisher
	Chong W, Mattapallil M, Raychaudhuri K, Bing S, Wu S, Zhong Y, et al. The Cytokine IL-17A Limits Th17 Pathogenicity via a Negative Feedback Loop Driven by Autocrine Induction of IL-24. Immunity. 2020;53:384-397.e5 pubmed publisher
	Kim M, Galan C, Hill A, Wu W, Fehlner Peach H, Song H, et al. Critical Role for the Microbiota in CX3CR1+ Intestinal Mononuclear Phagocyte Regulation of Intestinal T Cell Responses. Immunity. 2018;49:151-163.e5 pubmed publisher
	Pachnio A, Ciáurriz M, Begum J, Lal N, Zuo J, Beggs A, et al. Cytomegalovirus Infection Leads to Development of High Frequencies of Cytotoxic Virus-Specific CD4+ T Cells Targeted to Vascular Endothelium. PLoS Pathog. 2016;12:e1005832 pubmed publisher
	Bal S, Bernink J, Nagasawa M, Groot J, Shikhagaie M, Golebski K, et al. IL-1?, IL-4 and IL-12 control the fate of group 2 innate lymphoid cells in human airway inflammation in the lungs. Nat Immunol. 2016;17:636-45 pubmed publisher
	Kroenke M, Eto D, Locci M, Cho M, Davidson T, Haddad E, et al. Bcl6 and Maf cooperate to instruct human follicular helper CD4 T cell differentiation. J Immunol. 2012;188:3734-44 pubmed publisher
	Iwamoto S, Iwai S, Tsujiyama K, Kurahashi C, Takeshita K, Naoe M, et al. TNF-alpha drives human CD14+ monocytes to differentiate into CD70+ dendritic cells evoking Th1 and Th17 responses. J Immunol. 2007;179:1449-57 pubmed
	Dzhagalov I, Chambon P, He Y. Regulation of CD8+ T lymphocyte effector function and macrophage inflammatory cytokine production by retinoic acid receptor gamma. J Immunol. 2007;178:2113-21 pubmed

product information

Antigen :

IL-4

Apps. Abbrev. :

ICFC

Cat # :

500826

Clone :

MP4-25D2

Item :

Brilliant Violet 421™ anti-human IL-4

Isotype :

Rat IgG1, κ

Other Names :

Interleukin-4, Ia inducing factor (IaIF), B-cell stimulating factor-1 (BSF-1), Hodgkin's cell growth factor (HCGF), Mast cell growth factor-2 (MCGF-2), Macrophage fusion factor (MFF), T cell growth factor-2 (TCGF-2)

Size :

100 tests

Price (USD) :

386 USD

Reactivity :

Human

Clonality :

Monoclonal

Host :

Rat

Conjugate/Tag/Label :

BV421

Immunogen :

CHO-expressed, recombinant human IL-4

Application Notes :

ELISA Detection^1,3 or ELISPOT Detection^4,5: The biotinylated MP4-25D2 antibody is useful as a detection antibody for a sandwich ELISA or ELISPOT assay, when used in conjunction with purified 8D4-8 antibody (Cat. No. 500702/500707) as the capture antibody.Flow Cytometry^6,9: The fluorochrome-labeled MP4-25D2 antibody is useful for intracellular immunofluorescent staining and flow cytometric analysis to identify IL-4 -producing cells within mixed cell populations.Neutralization^1-3: The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for neutralization of human IL-4 bioactivity (Cat. No. 500815). The MP4-25D2 antibody can neutralize the bioactivity of natural or recombinant IL-4.;

more info or order :

BioLegend product webpage