LEAF™ Purified anti-human IL-4 | BioLegend 500815 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

BioLegend

other brands :

Babco, Signet, Sternberger Monoclonals, Senetek, Covance

product type :

antibody

product name :

LEAF™ Purified anti-human IL-4

catalog :

500815

quantity :

500 μg

price :

250 USD

clonality :

monoclonal

host :

rat

conjugate :

nonconjugated

clone name :

MP4-25D2

reactivity :

human, Rhesus monkey

application :

immunohistochemistry, immunocytochemistry, neutralization, flow cytometry

citations: 11

Published Application/Species/Sample/Dilution	Reference
flow cytometry; human; loading ...; tbl 1	Sumatoh H, Teng K, Cheng Y, Newell E. Optimization of mass cytometry sample cryopreservation after staining. Cytometry A. 2017;91:48-61 pubmed publisher
immunocytochemistry; human	Yu C, Becker C, Metang P, Marches F, Wang Y, Toshiyuki H, et al. Human CD141+ dendritic cells induce CD4+ T cells to produce type 2 cytokines. J Immunol. 2014;193:4335-43 pubmed publisher
flow cytometry; human	Weist B, Schmueck M, Fuehrer H, Sattler A, Reinke P, Babel N. The role of CD4(+) T cells in BKV-specific T cell immunity. Med Microbiol Immunol. 2014;203:395-408 pubmed publisher
	Logtenberg M, Jansen J, Raaben M, Toebes M, Franke K, Brandsma A, et al. Glutaminyl cyclase is an enzymatic modifier of the CD47- SIRPα axis and a target for cancer immunotherapy. Nat Med. 2019;25:612-619 pubmed publisher
	Yugo D, Heffron C, Ryu J, Uh K, Subramaniam S, Matzinger S, et al. Infection Dynamics of Hepatitis E Virus in Wild-Type and Immunoglobulin Heavy Chain Knockout JH -/- Gnotobiotic Piglets. J Virol. 2018;92: pubmed publisher
	Na H, Lim H, Choi G, Kim B, Kim S, Chang Y, et al. Concomitant suppression of TH2 and TH17 cell responses in allergic asthma by targeting retinoic acid receptor-related orphan receptor ?t. J Allergy Clin Immunol. 2018;141:2061-2073.e5 pubmed publisher
	Das A, Rouault Pierre K, Kamdar S, Gomez Tourino I, Wood K, Donaldson I, et al. Adaptive from Innate: Human IFN-Î³+CD4+ T Cells Can Arise Directly from CXCL8-Producing Recent Thymic Emigrants in Babies and Adults. J Immunol. 2017;199:1696-1705 pubmed publisher
	Sulzer D, Alcalay R, Garretti F, Cote L, Kanter E, Agin Liebes J, et al. T cells from patients with Parkinson's disease recognize α-synuclein peptides. Nature. 2017;546:656-661 pubmed publisher
	Cao D, Cao Q, Subramaniam S, Yugo D, Heffron C, Rogers A, et al. Pig model mimicking chronic hepatitis E virus infection in immunocompromised patients to assess immune correlates during chronicity. Proc Natl Acad Sci U S A. 2017;114:6914-6923 pubmed publisher
	Buranapraditkun S, Pissani F, Teigler J, Schultz B, Alter G, Marovich M, et al. Preservation of Peripheral T Follicular Helper Cell Function in HIV Controllers. J Virol. 2017;91: pubmed publisher
	Kato H, Perl A. Mechanistic target of rapamycin complex 1 expands Th17 and IL-4+ CD4-CD8- double-negative T cells and contracts regulatory T cells in systemic lupus erythematosus. J Immunol. 2014;192:4134-44 pubmed publisher

product information

Antigen :

IL-4

Apps. Abbrev. :

ELISA, CyTOF®, Neut, Intracellular Staining for Flow Cytometry, IHC

Cat # :

500815

Clone :

MP4-25D2

Item :

LEAF™ Purified anti-human IL-4

Isotype :

Rat IgG1, κ

Other Names :

Interleukin-4, Ia inducing factor (IaIF), B-cell stimulating factor-1 (BSF-1), Hodgkin's cell growth factor (HCGF), Mast cell growth factor-2 (MCGF-2), Macrophage fusion factor (MFF), T cell growth factor-2 (TCGF-2)

Size :

500 μg

Price (USD) :

250 USD

Reactivity :

Human, Cross-Reactivity: Swine (Pig, Porcine), Rhesus

Clonality :

Monoclonal

Host :

Rat

Conjugate/Tag/Label :

LEAF

Immunogen :

CHO-expressed, recombinant human IL-4

Application Notes :

ELISA Detection 1,3 or ELISPOT Detection 4,5 : The biotinylated MP4-25D2 antibody is useful as a detection antibody for a sandwich ELISA or ELISPOT assay, when used in conjunction with purified 8D4-8 antibody (Cat. No. 500702/500707) as the capture antibody. Flow Cytometry 6,9 : The fluorochrome-labeled MP4-25D2 antibody is useful for intracellular immunofluorescent staining and flow cytometric analysis to identify IL-4 -producing cells within mixed cell populations. Neutralization 1-3 : The LEAF purified antibody (Endotoxin 0.1 EU/ug, Azide-Free, 0.2 um filtered) is recommended for neutralization of human IL-4 bioactivity (Cat. No. 500815). The MP4-25D2 antibody can neutralize the bioactivity of natural or recombinant IL-4.