Brilliant Violet 421™ anti-human IL-2 | BioLegend 500328 product information

product summary

company name :

BioLegend

other brands :

Babco, Signet, Sternberger Monoclonals, Senetek, Covance

product type :

antibody

product name :

Brilliant Violet 421™ anti-human IL-2

catalog :

500328

quantity :

100 tests

price :

376 USD

clonality :

monoclonal

host :

rat

conjugate :

BV421

clone name :

MQ1-17H12

reactivity :

human

application :

immunocytochemistry, flow cytometry

more info or order :

BioLegend product webpage

citations: 19

Published Application/Species/Sample/Dilution	Reference
flow cytometry; human; 1:100; loading ...; fig 2e	Wagner D, Amini L, Wendering D, Burkhardt L, Akyüz L, Reinke P, et al. High prevalence of Streptococcus pyogenes Cas9-reactive T cells within the adult human population. Nat Med. 2019;25:242-248 pubmed publisher
flow cytometry; human; loading ...; fig 6b	Zhang C, Peng Y, Hublitz P, Zhang H, Dong T. Genetic abrogation of immune checkpoints in antigen-specific cytotoxic T-lymphocyte as a potential alternative to blockade immunotherapy. Sci Rep. 2018;8:5549 pubmed publisher
	Yin Z, Chen J, Lu Y, Wang B, Godfrey L, Mentzer A, et al. Evaluation of T cell responses to naturally processed variant SARS-CoV-2 spike antigens in individuals following infection or vaccination. Cell Rep. 2023;42:112470 pubmed publisher
	Zhang B, Sun J, Yuan Y, Ji D, Sun Y, Liu Y, et al. Proximity-enabled covalent binding of IL-2 to IL-2Rα selectively activates regulatory T cells and suppresses autoimmunity. Signal Transduct Target Ther. 2023;8:28 pubmed publisher
	Seenappa L, Jakubowski A, Steinbuck M, Palmer E, Haqq C, Carter C, et al. Amphiphile-CpG vaccination induces potent lymph node activation and COVID-19 immunity in mice and non-human primates. NPJ Vaccines. 2022;7:128 pubmed publisher
	Sung B, Lin Y, Kong Q, Shah P, Glick Bieler J, Palmer S, et al. Wnt activation promotes memory T cell polyfunctionality via epigenetic regulator PRMT1. J Clin Invest. 2022;132: pubmed publisher
	Apostolidis S, Kakara M, Painter M, Goel R, Mathew D, Lenzi K, et al. Cellular and humoral immune responses following SARS-CoV-2 mRNA vaccination in patients with multiple sclerosis on anti-CD20 therapy. Nat Med. 2021;27:1990-2001 pubmed publisher
	Peluso M, Deitchman A, Torres L, Iyer N, Munter S, Nixon C, et al. Long-term SARS-CoV-2-specific immune and inflammatory responses in individuals recovering from COVID-19 with and without post-acute symptoms. Cell Rep. 2021;36:109518 pubmed publisher
	Holokai L, Chakrabarti J, Lundy J, Croagh D, Adhikary P, Richards S, et al. Murine- and Human-Derived Autologous Organoid/Immune Cell Co-Cultures as Pre-Clinical Models of Pancreatic Ductal Adenocarcinoma. Cancers (Basel). 2020;12: pubmed publisher
	Peng Y, Mentzer A, Liu G, Yao X, Yin Z, Dong D, et al. Broad and strong memory CD4+ and CD8+ T cells induced by SARS-CoV-2 in UK convalescent individuals following COVID-19. Nat Immunol. 2020;21:1336-1345 pubmed publisher
	Wu W, Zhou Q, Masubuchi T, Shi X, Li H, Xu X, et al. Multiple Signaling Roles of CD3ε and Its Application in CAR-T Cell Therapy. Cell. 2020;182:855-871.e23 pubmed publisher
	Bartolom xe9 Casado R, Landsverk O, Chauhan S, S xe6 tre F, Hagen K, Yaqub S, et al. CD4+ T cells persist for years in the human small intestine and display a TH1 cytokine profile. Mucosal Immunol. 2021;14:402-410 pubmed publisher
	Sagnella S, Yang L, Stubbs G, Boslem E, Martino Echarri E, Smolarczyk K, et al. Cyto-Immuno-Therapy for Cancer: A Pathway Elicited by Tumor-Targeted, Cytotoxic Drug-Packaged Bacterially Derived Nanocells. Cancer Cell. 2020;37:354-370.e7 pubmed publisher
	Schreurs R, Baumdick M, Sagebiel A, Kaufmann M, Mokry M, Klarenbeek P, et al. Human Fetal TNF-α-Cytokine-Producing CD4+ Effector Memory T Cells Promote Intestinal Development and Mediate Inflammation Early in Life. Immunity. 2019;50:462-476.e8 pubmed publisher
	Dillinger B, Ahmadi Erber S, Soukup K, Halfmann A, Schrom S, Vanhove B, et al. CD28 Blockade Ex Vivo Induces Alloantigen-Specific Immune Tolerance but Preserves T-Cell Pathogen Reactivity. Front Immunol. 2017;8:1152 pubmed publisher
	Wu Z, Frascaroli G, Bayer C, Schmal T, Mertens T. Interleukin-2 from Adaptive T Cells Enhances Natural Killer Cell Activity against Human Cytomegalovirus-Infected Macrophages. J Virol. 2015;89:6435-41 pubmed publisher
	Boyle M, Jagannathan P, Bowen K, McIntyre T, Vance H, Farrington L, et al. Effector Phenotype of Plasmodium falciparum-Specific CD4+ T Cells Is Influenced by Both Age and Transmission Intensity in Naturally Exposed Populations. J Infect Dis. 2015;212:416-25 pubmed publisher
	Colleton B, Huang X, Melhem N, Fan Z, Borowski L, Rappocciolo G, et al. Primary human immunodeficiency virus type 1-specific CD8+ T-cell responses induced by myeloid dendritic cells. J Virol. 2009;83:6288-99 pubmed publisher
	Dzhagalov I, Chambon P, He Y. Regulation of CD8+ T lymphocyte effector function and macrophage inflammatory cytokine production by retinoic acid receptor gamma. J Immunol. 2007;178:2113-21 pubmed

product information

Antigen :

IL-2

Apps. Abbrev. :

ICFC

Cat # :

500328

Clone :

MQ1-17H12

Item :

Brilliant Violet 421™ anti-human IL-2

Isotype :

Rat IgG2a, κ

Other Names :

Interleukin-2, T cell growth factor (TCGF), Eosinophil differentiation factor (EDF), Killer cell helper factor (KHF), Macrophage-activating factor for cytotoxicity I (MAF-C I), Thymocyte differentiation factor (TDF)

Size :

100 tests

Price (USD) :

376 USD

Reactivity :

Human

Clonality :

Monoclonal

Host :

Rat

Conjugate/Tag/Label :

BV421

Immunogen :

E. coli - expressed recombinant human IL-2

Application Notes :

ELISA or ELISPOT Capture^2,3: The purified MQ1-17H12 antibody is useful as the capture antibody in a sandwich ELISA or ELISPOT assay, when used in conjunction with the Biotin anti-human IL-2 antibody (Cat. No. 517605) as the detecting antibody. The Ultra-LEAF™ purified antibody is suggested for ELISPOT capture. For ELISPOT capture applications, a concentration range of 4.0 - 8.0 µg/mL is recommended.Additional reported applications (for the relevant formats) include: immunoprecipitation^2, immunohistochemical staining of paraformaldehyde-fixed, saponin-treated frozen tissue sections^1,4-6,8, neutralization^13, and immunocytochemistry.Note: For testing human IL-2 in serum or plasma, BioLegend's LEGEND MAX™ Kit (Cat. No. 431807) is specially developed and recommended.Clone MQ1-17H12 cross-reacts to Cat^15;

more info or order :

BioLegend product webpage