product summary
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company name :
BioLegend
other brands :
Babco, Signet, Sternberger Monoclonals, Senetek, Covance
product type :
antibody
product name :
Alexa Fluor® 700 anti-human IL-2
catalog :
500319
quantity :
25 μg
price :
124 USD
clonality :
monoclonal
host :
rat
conjugate :
AF700
clone name :
MQ1-17H12
reactivity :
human
application :
immunocytochemistry, flow cytometry, mass cytometry
more info or order :
citations: 38
Published Application/Species/Sample/DilutionReference
  • mass cytometry; human; loading ...; fig s1a
  • flow cytometry; human; loading ...; fig 2d
Ingelfinger F, Krishnarajah S, Kramer M, Utz S, Galli E, Lutz M, et al. Single-cell profiling of myasthenia gravis identifies a pathogenic T cell signature. Acta Neuropathol. 2021;141:901-915 pubmed publisher
  • flow cytometry; human; 1:200; loading ...; fig s5
Cao W, Fang F, Gould T, Li X, Kim C, Gustafson C, et al. Ecto-NTPDase CD39 is a negative checkpoint that inhibits follicular helper cell generation. J Clin Invest. 2020;130:3422-3436 pubmed publisher
  • flow cytometry; human
Marotte L, Simon S, Vignard V, Dupré E, Gantier M, Cruard J, et al. Increased antitumor efficacy of PD-1-deficient melanoma-specific human lymphocytes. J Immunother Cancer. 2020;8: pubmed publisher
  • flow cytometry; human; loading ...; fig 6d
Lynn R, Weber E, Sotillo E, Gennert D, Xu P, Good Z, et al. c-Jun overexpression in CAR T cells induces exhaustion resistance. Nature. 2019;576:293-300 pubmed publisher
  • flow cytometry; human; loading ...; fig 1b
Meckiff B, Ladell K, McLaren J, Ryan G, Leese A, James E, et al. Primary EBV Infection Induces an Acute Wave of Activated Antigen-Specific Cytotoxic CD4+ T Cells. J Immunol. 2019;203:1276-1287 pubmed publisher
  • flow cytometry; human; loading ...; fig 3e
Canete P, Sweet R, Gonzalez Figueroa P, Papa I, Ohkura N, Bolton H, et al. Regulatory roles of IL-10-producing human follicular T cells. J Exp Med. 2019;: pubmed publisher
  • flow cytometry; human; loading ...; fig 1b
Amelio P, Portevin D, Hella J, Reither K, Kamwela L, Lweno O, et al. HIV Infection Functionally Impairs Mycobacterium tuberculosis-Specific CD4 and CD8 T-Cell Responses. J Virol. 2019;93: pubmed publisher
  • flow cytometry; human; loading ...; fig 6b
Zhang C, Peng Y, Hublitz P, Zhang H, Dong T. Genetic abrogation of immune checkpoints in antigen-specific cytotoxic T-lymphocyte as a potential alternative to blockade immunotherapy. Sci Rep. 2018;8:5549 pubmed publisher
  • flow cytometry; human; loading ...; fig 5a
Xie L, Zhou F, Liu X, Fang Y, Yu Z, Song N, et al. Serum microRNA181a: Correlates with the intracellular cytokine levels and a potential biomarker for acute graft-versus-host disease. Cytokine. 2016;85:37-44 pubmed publisher
Kramer K, Wilfong E, Voss K, Barone S, Shiakolas A, Raju N, et al. Single-cell profiling of the antigen-specific response to BNT162b2 SARS-CoV-2 RNA vaccine. Nat Commun. 2022;13:3466 pubmed publisher
O Connor R, Chauhan V, Mathieson L, Titmarsh H, Koppensteiner L, Young I, et al. T cells drive negative feedback mechanisms in cancer associated fibroblasts, promoting expression of co-inhibitory ligands, CD73 and IL-27 in non-small cell lung cancer. Oncoimmunology. 2021;10:1940675 pubmed publisher
Rodda L, Netland J, Shehata L, Pruner K, Morawski P, Thouvenel C, et al. Functional SARS-CoV-2-Specific Immune Memory Persists after Mild COVID-19. Cell. 2021;184:169-183.e17 pubmed publisher
Rakshit S, Hingankar N, Alampalli S, Adiga V, Sundararaj B, Sahoo P, et al. HIV Skews a Balanced Mtb-Specific Th17 Response in Latent Tuberculosis Subjects to a Pro-inflammatory Profile Independent of Viral Load. Cell Rep. 2020;33:108451 pubmed publisher
Rydyznski Moderbacher C, Ramirez S, Dan J, Grifoni A, Hastie K, Weiskopf D, et al. Antigen-Specific Adaptive Immunity to SARS-CoV-2 in Acute COVID-19 and Associations with Age and Disease Severity. Cell. 2020;183:996-1012.e19 pubmed publisher
Bailur J, McCachren S, Pendleton K, Vasquez J, Lim H, Duffy A, et al. Risk-associated alterations in marrow T cells in pediatric leukemia. JCI Insight. 2020;5: pubmed publisher
Karlsson J, Nilsson L, Mitra S, Alsén S, Shelke G, Sah V, et al. Molecular profiling of driver events in metastatic uveal melanoma. Nat Commun. 2020;11:1894 pubmed publisher
Pauthner M, Nkolola J, Havenar Daughton C, Murrell B, Reiss S, Bastidas R, et al. Vaccine-Induced Protection from Homologous Tier 2 SHIV Challenge in Nonhuman Primates Depends on Serum-Neutralizing Antibody Titers. Immunity. 2019;50:241-252.e6 pubmed publisher
Bray C, Wright D, Haupt S, Thomas S, Stauss H, Zamoyska R. Crispr/Cas Mediated Deletion of PTPN22 in Jurkat T Cells Enhances TCR Signaling and Production of IL-2. Front Immunol. 2018;9:2595 pubmed publisher
Voltersen V, Blango M, Herrmann S, Schmidt F, Heinekamp T, Strassburger M, et al. Proteome Analysis Reveals the Conidial Surface Protein CcpA Essential for Virulence of the Pathogenic Fungus Aspergillus fumigatus. MBio. 2018;9: pubmed publisher
Boutboul D, Kuehn H, Van de Wyngaert Z, Niemela J, Callebaut I, Stoddard J, et al. Dominant-negative IKZF1 mutations cause a T, B, and myeloid cell combined immunodeficiency. J Clin Invest. 2018;128:3071-3087 pubmed publisher
Ramsingh A, Gray S, Reilly A, Koday M, Bratt D, Koday M, et al. Sustained AAV9-mediated expression of a non-self protein in the CNS of non-human primates after immunomodulation. PLoS ONE. 2018;13:e0198154 pubmed publisher
Miles J, Tan M, Dolton G, Edwards E, Galloway S, Laugel B, et al. Peptide mimic for influenza vaccination using nonnatural combinatorial chemistry. J Clin Invest. 2018;128:1569-1580 pubmed publisher
Teigler J, Zelinskyy G, Eller M, Bolton D, Marovich M, Gordon A, et al. Differential Inhibitory Receptor Expression on T Cells Delineates Functional Capacities in Chronic Viral Infection. J Virol. 2017;91: pubmed publisher
Das A, Rouault Pierre K, Kamdar S, Gomez Tourino I, Wood K, Donaldson I, et al. Adaptive from Innate: Human IFN-γ+CD4+ T Cells Can Arise Directly from CXCL8-Producing Recent Thymic Emigrants in Babies and Adults. J Immunol. 2017;199:1696-1705 pubmed publisher
Termini J, Magnani D, Maxwell H, Lauer W, Castro I, Pecotte J, et al. Simian T Lymphotropic Virus 1 Infection of Papio anubis: tax Sequence Heterogeneity and T Cell Recognition. J Virol. 2017;91: pubmed publisher
Ott P, Hu Z, Keskin D, Shukla S, Sun J, Bozym D, et al. An immunogenic personal neoantigen vaccine for patients with melanoma. Nature. 2017;547:217-221 pubmed publisher
Whitfield S, Taylor C, Risdall J, Griffiths G, Jones J, Williamson E, et al. Interference of the T Cell and Antigen-Presenting Cell Costimulatory Pathway Using CTLA4-Ig (Abatacept) Prevents Staphylococcal Enterotoxin B Pathology. J Immunol. 2017;198:3989-3998 pubmed publisher
Pillet S, Aubin E, Trépanier S, Bussière D, Dargis M, Poulin J, et al. A plant-derived quadrivalent virus like particle influenza vaccine induces cross-reactive antibody and T cell response in healthy adults. Clin Immunol. 2016;168:72-87 pubmed publisher
Wu Z, Frascaroli G, Bayer C, Schmal T, Mertens T. Interleukin-2 from Adaptive T Cells Enhances Natural Killer Cell Activity against Human Cytomegalovirus-Infected Macrophages. J Virol. 2015;89:6435-41 pubmed publisher
Li J, Sun W, Subrahmanyam P, Page C, Younger K, Tiper I, et al. NKT Cell Responses to B Cell Lymphoma. Med Sci (Basel). 2014;2:82-97 pubmed
Gironi M, Saresella M, Rovaris M, Vaghi M, Nemni R, Clerici M, et al. A novel data mining system points out hidden relationships between immunological markers in multiple sclerosis. Immun Ageing. 2013;10:1 pubmed publisher
Qi Y, Operario D, Georas S, Mosmann T. The acute environment, rather than T cell subset pre-commitment, regulates expression of the human T cell cytokine amphiregulin. PLoS ONE. 2012;7:e39072 pubmed publisher
Vagenas P, Aravantinou M, Williams V, Jasny E, Piatak M, Lifson J, et al. A tonsillar PolyICLC/AT-2 SIV therapeutic vaccine maintains low viremia following antiretroviral therapy cessation. PLoS ONE. 2010;5:e12891 pubmed publisher
Masek Hammerman K, Li H, Liu J, Abbink P, La Porte A, O Brien K, et al. Mucosal trafficking of vector-specific CD4+ T lymphocytes following vaccination of rhesus monkeys with adenovirus serotype 5. J Virol. 2010;84:9810-6 pubmed publisher
Kassu A, Marcus R, D Souza M, Kelly McKnight E, Golden Mason L, Akkina R, et al. Regulation of virus-specific CD4+ T cell function by multiple costimulatory receptors during chronic HIV infection. J Immunol. 2010;185:3007-18 pubmed publisher
Maksaereekul S, Dubie R, Shen X, Kieu H, Dean G, Sparger E. Vaccination with vif-deleted feline immunodeficiency virus provirus, GM-CSF, and TNF-alpha plasmids preserves global CD4 T lymphocyte function after challenge with FIV. Vaccine. 2009;27:3754-65 pubmed publisher
Colleton B, Huang X, Melhem N, Fan Z, Borowski L, Rappocciolo G, et al. Primary human immunodeficiency virus type 1-specific CD8+ T-cell responses induced by myeloid dendritic cells. J Virol. 2009;83:6288-99 pubmed publisher
Dzhagalov I, Chambon P, He Y. Regulation of CD8+ T lymphocyte effector function and macrophage inflammatory cytokine production by retinoic acid receptor gamma. J Immunol. 2007;178:2113-21 pubmed
product information
Antigen :
IL-2
Apps. Abbrev. :
ICFC
Cat # :
500319
Clone :
MQ1-17H12
Item :
Alexa Fluor® 700 anti-human IL-2
Isotype :
Rat IgG2a, κ
Other Names :
Interleukin-2, T cell growth factor (TCGF), Eosinophil differentiation factor (EDF), Killer cell helper factor (KHF), Macrophage-activating factor for cytotoxicity I (MAF-C I), Thymocyte differentiation factor (TDF)
Size :
25 μg
Price (USD) :
124 USD
Reactivity :
Human
Clonality :
Monoclonal
Host :
Rat
Conjugate/Tag/Label :
A700
Immunogen :
E. coli - expressed recombinant human IL-2
Application Notes :
ELISA or ELISPOT Capture^2,3: The purified MQ1-17H12 antibody is useful as the capture antibody in a sandwich ELISA or ELISPOT assay, when used in conjunction with the Biotin anti-human IL-2 antibody (Cat. No. 517605) as the detecting antibody. The Ultra-LEAF™ purified antibody is suggested for ELISPOT capture. For ELISPOT capture applications, a concentration range of 4.0 - 8.0 µg/mL is recommended.Additional reported applications (for the relevant formats) include: immunoprecipitation^2, immunohistochemical staining of paraformaldehyde-fixed, saponin-treated frozen tissue sections^1,4-6,8, neutralization^13, and immunocytochemistry.Note: For testing human IL-2 in serum or plasma, BioLegend's LEGEND MAX™ Kit (Cat. No. 431807) is specially developed and recommended.Clone MQ1-17H12 cross-reacts to Cat^15;
more info or order :
company information
BioLegend
8999 BioLegend Way
San Diego, CA 92121
customerserv@biolegend.com
https://www.biolegend.com
headquarters: USA