LEAF™ Purified anti-human IL-2 | BioLegend 500313 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

BioLegend

other brands :

Babco, Signet, Sternberger Monoclonals, Senetek, Covance

product type :

antibody

product name :

LEAF™ Purified anti-human IL-2

catalog :

500313

quantity :

500 μg

price :

280 USD

clonality :

monoclonal

host :

rat

conjugate :

nonconjugated

clone name :

MQ1-17H12

reactivity :

human, rhesus macaque

application :

immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry

citations: 25

Published Application/Species/Sample/Dilution	Reference
flow cytometry; human; loading ...; fig 1b	Meckiff B, Ladell K, McLaren J, Ryan G, Leese A, James E, et al. Primary EBV Infection Induces an Acute Wave of Activated Antigen-Specific Cytotoxic CD4+ T Cells. J Immunol. 2019;203:1276-1287 pubmed publisher
flow cytometry; human; loading ...; fig 3e	Canete P, Sweet R, Gonzalez Figueroa P, Papa I, Ohkura N, Bolton H, et al. Regulatory roles of IL-10-producing human follicular T cells. J Exp Med. 2019;: pubmed publisher
flow cytometry; human; loading ...; fig 1b	Amelio P, Portevin D, Hella J, Reither K, Kamwela L, Lweno O, et al. HIV Infection Functionally Impairs Mycobacterium tuberculosis-Specific CD4 and CD8 T-Cell Responses. J Virol. 2019;93: pubmed publisher
flow cytometry; human; loading ...; fig 6b	Zhang C, Peng Y, Hublitz P, Zhang H, Dong T. Genetic abrogation of immune checkpoints in antigen-specific cytotoxic T-lymphocyte as a potential alternative to blockade immunotherapy. Sci Rep. 2018;8:5549 pubmed publisher
flow cytometry; human; loading ...; fig 2j	Pizzolla A, Nguyen T, Sant S, Jaffar J, Loudovaris T, Mannering S, et al. Influenza-specific lung-resident memory T cells are proliferative and polyfunctional and maintain diverse TCR profiles. J Clin Invest. 2018;128:721-733 pubmed publisher
flow cytometry; rhesus macaque; loading ...; fig 3a	Mylvaganam G, Rios D, Abdelaal H, Iyer S, Tharp G, Mavigner M, et al. Dynamics of SIV-specific CXCR5+ CD8 T cells during chronic SIV infection. Proc Natl Acad Sci U S A. 2017;114:1976-1981 pubmed publisher
flow cytometry; rhesus macaque; loading ...; tbl 1	Stanfield B, Pahar B, Chouljenko V, Veazey R, Kousoulas K. Vaccination of rhesus macaques with the live-attenuated HSV-1 vaccine VC2 stimulates the proliferation of mucosal T cells and germinal center responses resulting in sustained production of highly neutralizing antibodies. Vaccine. 2017;35:536-543 pubmed publisher
flow cytometry; human; loading ...	Wahid R, Fresnay S, Levine M, Sztein M. Cross-reactive multifunctional CD4+ T cell responses against Salmonella enterica serovars Typhi, Paratyphi A and Paratyphi B in humans following immunization with live oral typhoid vaccine Ty21a. Clin Immunol. 2016;173:87-95 pubmed publisher
flow cytometry; human; loading ...; fig 5a	Xie L, Zhou F, Liu X, Fang Y, Yu Z, Song N, et al. Serum microRNA181a: Correlates with the intracellular cytokine levels and a potential biomarker for acute graft-versus-host disease. Cytokine. 2016;85:37-44 pubmed publisher
immunocytochemistry; human; fig 6	Schnorfeil F, Lichtenegger F, Emmerig K, Schlueter M, Neitz J, Draenert R, et al. T cells are functionally not impaired in AML: increased PD-1 expression is only seen at time of relapse and correlates with a shift towards the memory T cell compartment. J Hematol Oncol. 2015;8:93 pubmed publisher
flow cytometry; human	Boer M, Prins C, van Meijgaarden K, van Dissel J, Ottenhoff T, Joosten S. Mycobacterium bovis BCG Vaccination Induces Divergent Proinflammatory or Regulatory T Cell Responses in Adults. Clin Vaccine Immunol. 2015;22:778-88 pubmed publisher
flow cytometry; human	Weist B, Schmueck M, Fuehrer H, Sattler A, Reinke P, Babel N. The role of CD4(+) T cells in BKV-specific T cell immunity. Med Microbiol Immunol. 2014;203:395-408 pubmed publisher
	Bray C, Wright D, Haupt S, Thomas S, Stauss H, Zamoyska R. Crispr/Cas Mediated Deletion of PTPN22 in Jurkat T Cells Enhances TCR Signaling and Production of IL-2. Front Immunol. 2018;9:2595 pubmed publisher
	Voltersen V, Blango M, Herrmann S, Schmidt F, Heinekamp T, Strassburger M, et al. Proteome Analysis Reveals the Conidial Surface Protein CcpA Essential for Virulence of the Pathogenic Fungus Aspergillus fumigatus. MBio. 2018;9: pubmed publisher
	Boutboul D, Kuehn H, Van de Wyngaert Z, Niemela J, Callebaut I, Stoddard J, et al. Dominant-negative IKZF1 mutations cause a T, B, and myeloid cell combined immunodeficiency. J Clin Invest. 2018;128:3071-3087 pubmed publisher
	Ramsingh A, Gray S, Reilly A, Koday M, Bratt D, Koday M, et al. Sustained AAV9-mediated expression of a non-self protein in the CNS of non-human primates after immunomodulation. PLoS ONE. 2018;13:e0198154 pubmed publisher
	Cuadrado E, van den Biggelaar M, de Kivit S, Chen Y, Slot M, Doubal I, et al. Proteomic Analyses of Human Regulatory T Cells Reveal Adaptations in Signaling Pathways that Protect Cellular Identity. Immunity. 2018;48:1046-1059.e6 pubmed publisher
	Miles J, Tan M, Dolton G, Edwards E, Galloway S, Laugel B, et al. Peptide mimic for influenza vaccination using nonnatural combinatorial chemistry. J Clin Invest. 2018;128:1569-1580 pubmed publisher
	Teigler J, Zelinskyy G, Eller M, Bolton D, Marovich M, Gordon A, et al. Differential Inhibitory Receptor Expression on T Cells Delineates Functional Capacities in Chronic Viral Infection. J Virol. 2017;91: pubmed publisher
	Das A, Rouault Pierre K, Kamdar S, Gomez Tourino I, Wood K, Donaldson I, et al. Adaptive from Innate: Human IFN-Î³+CD4+ T Cells Can Arise Directly from CXCL8-Producing Recent Thymic Emigrants in Babies and Adults. J Immunol. 2017;199:1696-1705 pubmed publisher
	Termini J, Magnani D, Maxwell H, Lauer W, Castro I, Pecotte J, et al. Simian T Lymphotropic Virus 1 Infection of Papio anubis: tax Sequence Heterogeneity and T Cell Recognition. J Virol. 2017;91: pubmed publisher
	Ott P, Hu Z, Keskin D, Shukla S, Sun J, Bozym D, et al. An immunogenic personal neoantigen vaccine for patients with melanoma. Nature. 2017;547:217-221 pubmed publisher
	Whitfield S, Taylor C, Risdall J, Griffiths G, Jones J, Williamson E, et al. Interference of the T Cell and Antigen-Presenting Cell Costimulatory Pathway Using CTLA4-Ig (Abatacept) Prevents Staphylococcal Enterotoxin B Pathology. J Immunol. 2017;198:3989-3998 pubmed publisher
	Wu Z, Frascaroli G, Bayer C, Schmal T, Mertens T. Interleukin-2 from Adaptive T Cells Enhances Natural Killer Cell Activity against Human Cytomegalovirus-Infected Macrophages. J Virol. 2015;89:6435-41 pubmed publisher
	Okano S, Yonemitsu Y, Shirabe K, Kakeji Y, Maehara Y, Harada M, et al. Provision of continuous maturation signaling to dendritic cells by RIG-I-stimulating cytosolic RNA synthesis of Sendai virus. J Immunol. 2011;186:1828-39 pubmed publisher

product information

Antigen :

IL-2

Apps. Abbrev. :

ELISA Capture, CyTOF®, ELISPOT Capture, Intracellular Staining for Flow Cytometry, IHC, IP

Cat # :

500313

Clone :

MQ1-17H12

Item :

LEAF™ Purified anti-human IL-2

Isotype :

Rat IgG2a, κ

Other Names :

Interleukin-2, T cell growth factor (TCGF), Eosinophil differentiation factor (EDF), Killer cell helper factor (KHF), Macrophage-activating factor for cytotoxicity I (MAF-C I), Thymocyte differentiation factor (TDF)

Size :

500 μg

Price (USD) :

280 USD

Reactivity :

Human, Cat (Feline), Cross-Reactivity: Chimpanzee, Baboon, Cynomolgus, Rhesus, Sooty Mangabey

Clonality :

Monoclonal

Host :

Rat

Conjugate/Tag/Label :

LEAF

Immunogen :

E. coli - expressed recombinant human IL-2

Application Notes :

ELISA or ELISPOT Capture 2,3 : The purified MQ1-17H12 antibody is useful as the capture antibody in a sandwich ELISA or ELISPOT assay, when used in conjunction with the Biotin anti-human IL-2 antibody (Cat. No. 517605) as the detecting antibody. The Ultra-LEAF™ purified antibody is suggested for ELISPOT capture. For ELISPOT capture applications, a concentration range of 4.0 - 8.0 µg/mL is recommended. Additional reported applications (for the relevant formats) include: immunoprecipitation 2 , immunohistochemical staining of paraformaldehyde-fixed, saponin-treated frozen tissue sections 1,4-6,8 , neutralization 13 , and immunocytochemistry. Note: For testing human IL-2 in serum or plasma, BioLegend's LEGEND MAX™ Kit (Cat. No. 431807) is specially developed and recommended.