LEAF™ Purified anti-human IL-1α | BioLegend 500102 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

BioLegend

other brands :

Babco, Signet, Sternberger Monoclonals, Senetek, Covance

product type :

antibody

product name :

LEAF™ Purified anti-human IL-1α

catalog :

500102

quantity :

500 μg

price :

280 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

364-3B3-14

reactivity :

African green monkey, human

application :

immunohistochemistry, flow cytometry

citations: 3

Published Application/Species/Sample/Dilution	Reference
flow cytometry; African green monkey; loading ...; fig 1i	Neumann B, Shi T, Gan L, Klippert A, Daskalaki M, Stolte Leeb N, et al. Comprehensive panel of cross-reacting monoclonal antibodies for analysis of different immune cells and their distribution in the common marmoset (Callithrix jacchus). J Med Primatol. 2016;45:139-46 pubmed publisher
	Boscolo Sesillo F, Wong M, Cortez A, Alperin M. Isolation of muscle stem cells from rat skeletal muscles. Stem Cell Res. 2020;43:101684 pubmed publisher
	Lu Y, Xue Q, Eisele M, Sulistijo E, Brower K, Han L, et al. Highly multiplexed profiling of single-cell effector functions reveals deep functional heterogeneity in response to pathogenic ligands. Proc Natl Acad Sci U S A. 2015;112:E607-15 pubmed publisher

product information

Antigen :

IL-1alpha

Apps. Abbrev. :

ELISA Capture, ELISPOT Capture, Intracellular Staining for Flow Cytometry, IHC

Cat # :

500102

Clone :

364-3B3-14

Item :

LEAF™ Purified anti-human IL-1α

Isotype :

Mouse IgG1, κ

Other Names :

Interleukin-1α, Lymphocyte-activating factor (LAF), Endogenous pyrogen (EP), Leukocyte endogenous mediator (LEM), Mononuclear cell factor (MCF/MNCF)

Size :

500 μg

Price (USD) :

280 USD

Reactivity :

Human, Cross-Reactivity: Rhesus

Clonality :

Monoclonal

Host :

Mouse

Conjugate/Tag/Label :

LEAF

Immunogen :

Recombinant human IL-1α

Application Notes :

ELISA or ELISPOT Capture 1 : The purified 364-3B3-14 antibody is useful as the capture antibody in a sandwich ELISA assay, when used in conjunction with the biotinylated Poly5313 antibody (Cat. No. 531304) as the detecting antibody and recombinant human IL-1a (Cat. No. 570009) as the standard. The Ultra-LEAF purified antibody (Cat. No. 500108) is suggested for ELISPOT capture. Flow Cytometry 4 : The fluorochrome-labeled 364-3B3-14 antibody is useful for intracellular immunofluorescent staining and flow cytometric analysis to identify IL-1a -producing cells within mixed cell populations. For intracellular cytokine staining protocol, please visit www.biolegend.com and click on the support section. Additional reported applications (for the relevant formats) include: immunohistochemistry 3 . The 364-3B3-14 is a non-neutralizing antibody.