Babco, Signet, Sternberger Monoclonals, Senetek, Covance

other

Apotracker™ Green

427402

100 tests

247 USD

427402

Apotracker™ Green

Fixable Dye, Fixable Viability Dye, Dead, Apoptosis

100 tests

247 USD

Human, Mouse

NONAB_CHMPRB

To reconstitute the reagent to an 80 µM stock concentration, add the following volumes:;Add 200 µL DMSO to the 200 test size;Add 100 µL DMSO to the 100 test size;Add 20 µL DMSO to the 20 test size; Take the appropriate volume of reconstitute Apotracker solution to perform a 1:10 dilution with cell staining buffer.;Add 5 µL of the diluted reagent to 1x10^6 cells in 100 µL of an appropriate buffer, like FACS staining buffer, to achieve a 400nM staining solution. However, different cell types might require optimization of the staining solution concentration. We recommend a 200-800nM range for cells in suspension.;Incubate at room temperature for 10-20 minutes.;Co-stain with an appropriate dead cell stain as needed.;Wash the cells at least 2 times.;Analyze immediately or within 1 hour of staining. Do not fix or store samples overnight. This reagent will be registered in the FITC channel of the flow cytometer.;