product summary
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company name :
BioLegend
other brands :
Babco, Signet, Sternberger Monoclonals, Senetek, Covance
product type :
other
product name :
Western-Ready™ Rapid Protein Extraction Buffer
catalog :
426305
quantity :
100 mL
price :
141 USD
more info or order :
product information
Apps. Abbrev. :
Western Blotting
Cat # :
426305
Item :
Western-Ready™ Rapid Protein Extraction Buffer
Other Names :
Lysis Buffer, Protein Buffer
Size :
100 mL
Price (USD) :
141 USD
Conjugate/Tag/Label :
WSTRN_RDY
Application Notes :
During product development testing, Western-Ready™ Rapid Protein Extraction Buffer displayed 20% higher protein extraction efficiency compared to RIPA buffer (containing 0.1% SDS). Due to the chaotropic and denaturing properties of Western-Ready™ Rapid Protein Extraction Buffer, it is not compatible with immunoprecipitation applications. Due to high temperatures required for using Western-Ready™ Rapid Protein Extraction Buffer, the reagent may not be compatible with the extraction of multi-pass transmembrane proteins that are prone to aggregation. Western-Ready™ Rapid Protein Extraction Buffer may solubilize DNA and cause the sample to become viscious. If this occurs, sonicate samples until the crude lysate can be easily drawn through a 200 µL pipette tip. When thawing samples from -20°C or -80°C freezers, SDS precipitates will be observed if kept on ice. To resolubilize SDS, briefly incubate tubes in a 37°C water bath. If Western-Ready™ Rapid Protein Extraction Buffer gets over-boiled it burns and its color changes from clear to yellowish. If this occurs, discard and boil new lysis buffer.
more info or order :
company information
BioLegend
8999 BioLegend Way
San Diego, CA 92121
San Diego, CA 92121
customerserv@biolegend.com
https://www.biolegend.comheadquarters: USA