DRAQ5™ | BioLegend 424101 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

BioLegend

other brands :

Babco, Signet, Sternberger Monoclonals, Senetek, Covance

product type :

ELISA/assay

product name :

DRAQ5™

catalog :

424101

quantity :

50 μL

price :

204 USD

citations: 11

Reference
Li Y, Ritzel R, Khan N, Cao T, He J, Lei Z, et al. Delayed microglial depletion after spinal cord injury reduces chronic inflammation and neurodegeneration in the brain and improves neurological recovery in male mice. Theranostics. 2020;10:11376-11403 pubmed publisher
Merlo L, Bowers J, Stefanoni T, Getts R, Mandik Nayak L. B-Cell-Targeted 3DNA Nanotherapy Against Indoleamine 2,3-Dioxygenase 2 (IDO2) Ameliorates Autoimmune Arthritis in a Preclinical Model. Clin Pathol. 2020;13:2632010X20951812 pubmed publisher
Webb A, Gao B, Goldsmith Z, Irvine A, Saleh N, Lee R, et al. Inhibition of MMP-2 and MMP-9 decreases cellular migration, and angiogenesis in in vitro models of retinoblastoma. BMC Cancer. 2017;17:434 pubmed publisher
Wen J, Tao W, Hao S, Zu Y. Cellular function reinstitution of offspring red blood cells cloned from the sickle cell disease patient blood post CRISPR genome editing. J Hematol Oncol. 2017;10:119 pubmed publisher
Sándor N, Lukácsi S, Ungai Salánki R, Orgovan N, Szabó B, Horvath R, et al. CD11c/CD18 Dominates Adhesion of Human Monocytes, Macrophages and Dendritic Cells over CD11b/CD18. PLoS ONE. 2016;11:e0163120 pubmed publisher
Jeong K, Hwang S, Oh G, Park J. FICZ, a tryptophan photoproduct, suppresses pulmonary eosinophilia and Th2-type cytokine production in a mouse model of ovalbumin-induced allergic asthma. Int Immunopharmacol. 2012;13:377-85 pubmed publisher
Perlot T, Penninger J. Development and function of murine B cells lacking RANK. J Immunol. 2012;188:1201-5 pubmed publisher
Stadnisky M, Xie X, Coats E, BULLOCK T, Brown M. Self MHC class I-licensed NK cells enhance adaptive CD8 T-cell viral immunity. Blood. 2011;117:5133-41 pubmed publisher
Charles N, Hardwick D, Daugas E, Illei G, Rivera J. Basophils and the T helper 2 environment can promote the development of lupus nephritis. Nat Med. 2010;16:701-7 pubmed publisher
Bankoti J, Burnett A, Navarro S, Miller A, Rase B, Shepherd D. Effects of TCDD on the fate of naive dendritic cells. Toxicol Sci. 2010;115:422-34 pubmed publisher
Hayashida K, Chen Y, Bartlett A, Park P. Syndecan-1 is an in vivo suppressor of Gram-positive toxic shock. J Biol Chem. 2008;283:19895-903 pubmed publisher

product information

Antigen :

DRAQ5

Apps. Abbrev. :

Flow Cytometry, Intracellular Staining for Flow Cytometry, ICC, IHC-F, IHC-P

Cat # :

424101

Item :

DRAQ5™

Other Names :

Live/Dead Staining, Dead cell exclusion dye, Cell viability dye

Size :

50 μL

Price (USD) :

204 USD

Conjugate/Tag/Label :

NONAB_CHMPRB

Application Notes :

DRAQ5 is a cell-permeant DNA binding anthraquinone dye that intercalates between A-T bases of dsDNA. Due to its cell permeability, this dye is useful for assessing DNA content and cell cycle but is not suitable to be used as a viability dye. In microscopy applications, DRAQ5 is also useful as a nuclear counterstain for both live and fixed specimens. It is a far-red emitting dye with optimal excitation/ emission peaks of 633nm/ 695nm, respectively, (when intercalated into DNA) that can be detected in the Alexa Fluor 647, APC and Alexa Fluor 700 channels. For use in cell cycle analysis, the optimal concentration must be determined for each cell type. Also, due to the wide emission spectrum and the brightness of DRAQ5 off the 633nm red laser and the dim emission intensity of the APC-Cy7 or APC-Fire 750 fluorophores, flow cytometric panels should be optimized to determine that including DRAQ5 in a panel is compatible with the use of the APC-Cy7 or APC-Fire 750 channel as well. Protocol for DNA staining using DRAQ5 : 1. Perform surface staining following protocol of choice. 2. Wash cells twice with phosphate buffered saline (PBS). Sodium azide interferes with DRAQ5 staining, thus it is recommended to stain in PBS (without calcium, magnesium, or sodium azide) or culture medium. 3. Dilute DRAQ5 to required concentration. We recommend titrating the reagent to determine optimal concentration for cells of interest. Good results in flow cytometry and microscopy applications have been observed using a 1:200 to 1:1000 dilution. 4. Incubate at room temperature, preventing exposure to light, for 10-15 minutes. Subsequent washing can help make the cell cycle profile more distinct but is not absolutely necessary since DRAQ5 is a fluorogenic reagent. 5. Analyze cells on a cytometer equipped with the 633 red laser. If performing cell cycle analysis, detection in the Alexa 700 channel with a 680LP or 715LP filter might help with resolution of the emission peaks.